FastQCFastQC Report
Thu 26 May 2016
SRR1512055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072315
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58660.2830650745663666No Hit
TATCAACGCAGAGTACTTTTTTTTT38750.18698894714365336No Hit
GGTATCAACGCAGAGTACTTTTTTT35700.1722711074329916No Hit
GGTATCAACGCAGAGTACATGGGGG27680.13357042727577614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC500.001505450813.2934137
GGTATCA14600.013.2058311
GTCCTAC1302.4301698E-911.7474561
TATGCCG907.432829E-611.615455
TGGTATC5200.010.9656342
GTATCAA32150.010.5096811
GTGGTAT5900.010.353691
TAGATTG856.5723044E-410.0625825
TTATACC1054.089179E-59.95614
GTATTAG1356.0424463E-79.898321
GTCTATG2700.09.898321
ATAACTG1856.4392225E-109.7604035
GTATTAA2650.09.7248991
GTCTAGT1002.6285936E-49.5448091
CTAGCAC1304.219164E-69.503553
ACCGCCC900.00111890129.4952958
CTGGCCG1252.770512E-59.1154839
CCGGTAC1151.1062513E-49.0903523
TAACGTT850.00741374968.9445175
GGACGAT850.00743727948.9410636
Produced by FastQC (version 0.11.5)