FastQCFastQC Report
Thu 26 May 2016
SRR1512054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248431
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT42160.1875085337286312No Hit
GTATCAACGCAGAGTACTTTTTTTT41540.18475105529144545No Hit
CTGTAGGACGTGGAATATGGCAAGA38860.1728316323694167No Hit
GTCCTAAAGTGTGTATTTCTCATTT34230.15223949500785214No Hit
CTTTAGGACGTGAAATATGGCGAGG33470.14885936014936638No Hit
TATCAACGCAGAGTACTTTTTTTTT23880.10620739529031578No Hit
GGTATCAACGCAGAGTACTTTTTTT23700.10540683703435863No Hit
GTCCTACAGTGTGCATTTCTCATTT23420.10416152419175861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1000.018.6133481
CGTCTAC558.433326E-616.0306361
CGTTAGG705.2880005E-613.9951
CGCATAC450.00939316613.0619991
TAGGACC8700.012.730454
TTCAACG852.7971528E-612.6778222
AGGACCG604.2180892E-412.6216415
TGGGCGA604.2180892E-412.62164110
GGTATCA11700.012.47588351
CGTATAT600.004674089611.429251
CTGGGCG752.1443446E-411.3594789
GGCGAGG10150.011.00532219
AGGACCT15250.010.61459355
TTAGACT2600.010.5584894
GTCCTAT2600.010.55007651
GTCAACG650.00847696510.55007652
AGGACGT25050.010.5432285
GTATCAA22950.010.4581371
GGACGTG24200.010.4441486
CTAGGAC3900.010.4371263
Produced by FastQC (version 0.11.5)