##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512054_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2248431 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.846941711798138 31.0 30.0 34.0 25.0 34.0 2 26.905513222331482 31.0 25.0 34.0 10.0 34.0 3 28.87734824862315 31.0 28.0 34.0 16.0 34.0 4 33.41216964185247 35.0 33.0 37.0 28.0 37.0 5 33.882593684218016 37.0 35.0 37.0 30.0 37.0 6 34.1728854476744 37.0 35.0 37.0 30.0 37.0 7 34.255373636104466 37.0 35.0 37.0 30.0 37.0 8 34.29518095062735 37.0 35.0 37.0 30.0 37.0 9 35.96156386386774 39.0 37.0 39.0 31.0 39.0 10 35.87171543178332 39.0 37.0 39.0 31.0 39.0 11 35.89215813160377 39.0 37.0 39.0 31.0 39.0 12 35.800532015436545 39.0 37.0 39.0 30.0 39.0 13 35.80428307562029 39.0 37.0 39.0 30.0 39.0 14 37.04227481296958 40.0 38.0 41.0 31.0 41.0 15 36.99129748700316 40.0 38.0 41.0 31.0 41.0 16 36.94939093083132 40.0 37.0 41.0 31.0 41.0 17 36.92337100849437 40.0 37.0 41.0 31.0 41.0 18 36.87697821280707 40.0 37.0 41.0 31.0 41.0 19 36.91367313473262 40.0 38.0 41.0 31.0 41.0 20 36.826466100138276 40.0 37.0 41.0 31.0 41.0 21 36.77440268347127 40.0 37.0 41.0 30.0 41.0 22 36.6826947324601 40.0 37.0 41.0 30.0 41.0 23 36.599113782010654 40.0 37.0 41.0 30.0 41.0 24 36.5249362777866 40.0 37.0 41.0 30.0 41.0 25 36.43544542838984 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1519721626369197 1101 2 3.9580215092486277 1101 3 2.140515291061945 1101 4 1.1779128769703888 1101 5 0.7367714421362521 1101 6 0.4261996749470214 1101 7 0.09691564681855169 1101 8 0.16966823355123495 1101 9 0.11300457304236744 1101 10 0.261481171090864 1101 11 0.2563510809123031 1101 12 0.24349030424705376 1101 13 0.23022163243823712 1101 14 -0.026696275228019317 1101 15 0.19220642595023207 1101 16 0.25997350395331864 1101 17 0.16443805420972524 1101 18 0.30761800965397157 1101 19 0.1720687179691751 1101 20 0.09979835051878894 1101 21 0.3523396834529038 1101 22 0.3481473027667903 1101 23 0.4311071856900881 1101 24 0.2611600788626376 1101 25 0.16449579462794617 1102 1 1.5668057574033902 1102 2 4.193934856660171 1102 3 2.5158447846382543 1102 4 1.6757248099408173 1102 5 1.077320870563696 1102 6 0.957768908792545 1102 7 0.73120648747134 1102 8 0.7717359197395979 1102 9 0.8695832981943994 1102 10 0.6609253385648017 1102 11 0.8200651068340363 1102 12 0.8009704670786917 1102 13 0.8364269878319348 1102 14 0.8287068789932093 1102 15 0.8640308239446952 1102 16 0.9381986228817496 1102 17 0.9391380816365569 1102 18 0.9210097364772807 1102 19 0.7227631033040609 1102 20 0.8698722948895963 1102 21 0.8266922142202731 1102 22 0.7691769305049192 1102 23 0.9261440092571718 1102 24 0.97775642204126 1102 25 0.9790734956646219 1103 1 1.2590722583424494 1103 2 4.0373487585399594 1103 3 2.417399935863614 1103 4 1.441425665961269 1103 5 1.1472663033587835 1103 6 0.9757320704462344 1103 7 0.6778451072591523 1103 8 0.6331108117897273 1103 9 0.6193141971211844 1103 10 0.742696235346159 1103 11 0.6648822212497123 1103 12 0.6201089223766445 1103 13 0.718653765826204 1103 14 0.7150075768862436 1103 15 0.8653858295503767 1103 16 0.5876067742393474 1103 17 0.8166327631309827 1103 18 0.8302825566500118 1103 19 0.804020661423273 1103 20 0.8989493254382452 1103 21 0.8811078495952103 1103 22 0.9127816840556733 1103 23 1.0797130484213895 1103 24 0.9776183157006741 1103 25 1.0597818856465295 1104 1 1.4341130019530866 1104 2 3.868320385859402 1104 3 2.430154873045826 1104 4 1.6567825837307595 1104 5 1.2406429912601311 1104 6 0.9938512626764933 1104 7 0.781109978027672 1104 8 0.7423678972714143 1104 9 0.8473971684218498 1104 10 0.8707152262428224 1104 11 0.7270633192482023 1104 12 0.773671450597945 1104 13 0.843257420834064 1104 14 0.9867672000771961 1104 15 0.8419589027978631 1104 16 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-0.7823057018643738 2315 11 -0.8524336359317672 2315 12 -0.6920761494263701 2315 13 -0.8059424967073667 2315 14 -0.8675850875198208 2315 15 -1.2039966368184238 2315 16 -1.0948947623805907 2315 17 -1.0316575302345683 2315 18 -1.0863115011451256 2315 19 -0.94372717792956 2315 20 -1.0818011131076517 2315 21 -0.9648842634158683 2315 22 -1.1637014916652788 2315 23 -1.1116219929297273 2315 24 -1.0990468350914426 2315 25 -1.0061802207622534 2316 1 -0.4952514490047264 2316 2 0.014982901873747068 2316 3 -0.053251803813452625 2316 4 -0.3668403530193274 2316 5 -0.6164754030303712 2316 6 -0.6586786502194428 2316 7 -0.8635466449463891 2316 8 -0.9673713289658892 2316 9 -0.8905542256674224 2316 10 -1.1393071678554776 2316 11 -1.1290997093785222 2316 12 -1.28749307999923 2316 13 -1.3299844345756853 2316 14 -1.3993511511051366 2316 15 -1.245631617970993 2316 16 -1.235804424380369 2316 17 -1.505112281020395 2316 18 -1.4613559567000678 2316 19 -1.4431264349575628 2316 20 -1.3954779274764917 2316 21 -1.4378834615627198 2316 22 -1.5142182686238854 2316 23 -1.5033196359299836 2316 24 -1.555082483487027 2316 25 -1.6103578785288022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59438.0 3 5.0 4 497.0 5 2384.0 6 3023.0 7 2624.0 8 2428.0 9 2391.0 10 2251.0 11 2186.0 12 2284.0 13 2206.0 14 2319.0 15 2427.0 16 2454.0 17 2798.0 18 3056.0 19 3827.0 20 5116.0 21 6468.0 22 7961.0 23 9475.0 24 11220.0 25 12925.0 26 14801.0 27 16937.0 28 20145.0 29 24693.0 30 31597.0 31 41200.0 32 54825.0 33 75108.0 34 106059.0 35 158415.0 36 261264.0 37 368862.0 38 922762.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.648820888877616 19.63280171817592 13.501726314928053 25.216651078018405 2 16.335615823608933 21.86472873622578 40.189641388052344 21.61001405211295 3 18.095640915820855 25.285543563489384 30.215336828214873 26.403478692474884 4 11.68130131634015 17.729963694683093 39.81282947975722 30.77590550921954 5 12.632497950793242 38.10230333952877 36.552066752326404 12.713131957351592 6 30.094199063346423 38.48641051756114 19.041375537597347 12.378014881495087 7 27.0278696566628 31.865420820118562 23.411970391797656 17.694739131420977 8 23.843782619969215 38.117691848226606 21.42462899684269 16.61389653496149 9 25.796877911752684 16.014144974873588 21.320111668981614 36.868865444392114 10 14.12114230818838 29.936666918694378 35.52968071054025 20.412510062577 11 33.13056971728285 23.00421938676348 25.753914618682984 18.111296277270682 12 22.653530395195585 25.189387621857197 32.867319477448945 19.289762505498278 13 28.175514392036042 22.836680333975114 26.44550800091264 22.542297273076205 14 19.943329370569966 23.118343413696042 28.721895401726805 28.216431814007194 15 21.871207077290787 32.18613335254673 24.025242491319503 21.91741707884298 16 20.933842310482287 29.053104142399743 29.125332287270545 20.88772125984742 17 20.71546780844064 29.38933860990175 28.916208680631073 20.978984901026536 18 20.37277699648909 28.356198258156162 31.156740149313606 20.114284596041134 19 22.597488545279393 27.635831059882992 28.404771710598297 21.36190868423932 20 22.566093019904628 28.21854616259759 29.179931525309748 20.03542929218803 21 22.08395415345339 27.579446443423457 28.00895760039495 22.3276418027282 22 22.02236616681403 29.05324506892914 28.497426609903236 20.426962154353593 23 21.367337762234964 28.668398554891827 28.68939113943924 21.27487254343397 24 22.083508084978444 27.663848847350753 29.612542802107605 20.6401002655632 25 21.853573881082927 28.16959790502147 29.01374473853686 20.96308347535874 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 193.0 1 193.0 2 407.5 3 622.0 4 622.0 5 622.0 6 1504.5 7 2387.0 8 2387.0 9 2387.0 10 3821.5 11 5256.0 12 5256.0 13 5256.0 14 9037.0 15 12818.0 16 12818.0 17 12818.0 18 22697.5 19 32577.0 20 32577.0 21 32577.0 22 51189.0 23 69801.0 24 69801.0 25 69801.0 26 98230.5 27 126660.0 28 126660.0 29 126660.0 30 162215.0 31 197770.0 32 197770.0 33 197770.0 34 221635.5 35 245501.0 36 245501.0 37 245501.0 38 259769.0 39 274037.0 40 274037.0 41 274037.0 42 282601.5 43 291166.0 44 291166.0 45 291166.0 46 290252.5 47 289339.0 48 289339.0 49 289339.0 50 272992.5 51 256646.0 52 256646.0 53 256646.0 54 221812.0 55 186978.0 56 186978.0 57 186978.0 58 155360.5 59 123743.0 60 123743.0 61 123743.0 62 98170.0 63 72597.0 64 72597.0 65 72597.0 66 54662.0 67 36727.0 68 36727.0 69 36727.0 70 26095.0 71 15463.0 72 15463.0 73 15463.0 74 10474.0 75 5485.0 76 5485.0 77 5485.0 78 3666.5 79 1848.0 80 1848.0 81 1848.0 82 1202.0 83 556.0 84 556.0 85 556.0 86 359.5 87 163.0 88 163.0 89 163.0 90 115.5 91 68.0 92 68.0 93 68.0 94 45.0 95 22.0 96 22.0 97 22.0 98 15.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.371417668587562 3 0.0 4 0.0 5 0.0 6 4.447545866428634E-5 7 0.0 8 0.0 9 0.0 10 4.447545866428634E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.002791279785771E-4 19 5.781809626357224E-4 20 4.002791279785771E-4 21 0.002713002978521467 22 0.001556641053250022 23 8.005582559571542E-4 24 8.895091732857267E-5 25 3.1132821065000437E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2248431.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.97437438371163 #Duplication Level Percentage of deduplicated Percentage of total 1 86.8581755114066 66.85853720101144 2 9.64946329289055 14.855228002176798 3 1.9959864574850044 4.60919426529807 4 0.5998645769839887 1.846968021131694 5 0.2669785029717857 1.027525162007655 6 0.16448405737527105 0.759663444753361 7 0.09808531172295469 0.5285038854274041 8 0.06082139033174215 0.3745350775946703 9 0.0454611163154961 0.3149406888453412 >10 0.22828426404811064 3.0865170926460728 >50 0.016888997530868984 0.9062385982819197 >100 0.012700724204713819 1.983535578833821 >500 0.0015782606622991 0.8876999766500637 >1k 0.0012275360706770185 1.9609130053416257 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4216 0.1875085337286312 No Hit GTATCAACGCAGAGTACTTTTTTTT 4154 0.18475105529144545 No Hit CTGTAGGACGTGGAATATGGCAAGA 3886 0.1728316323694167 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3423 0.15223949500785214 No Hit CTTTAGGACGTGAAATATGGCGAGG 3347 0.14885936014936638 No Hit TATCAACGCAGAGTACTTTTTTTTT 2388 0.10620739529031578 No Hit GGTATCAACGCAGAGTACTTTTTTT 2370 0.10540683703435863 No Hit GTCCTACAGTGTGCATTTCTCATTT 2342 0.10416152419175861 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.3342637599285902E-4 0.0 4 0.0 0.0 0.0 1.3342637599285902E-4 0.0 5 0.0 0.0 0.0 1.3342637599285902E-4 0.0 6 0.0 0.0 0.0 1.3342637599285902E-4 0.0 7 0.0 0.0 0.0 1.3342637599285902E-4 0.0 8 0.0 0.0 0.0 1.3342637599285902E-4 0.0 9 0.0 0.0 0.0 1.3342637599285902E-4 0.0 10 0.0 0.0 0.0 1.3342637599285902E-4 0.0 11 0.0 0.0 0.0 2.223772933214317E-4 0.0 12 0.0 0.0 0.0 2.223772933214317E-4 0.0 13 0.0 0.0 0.0 2.6685275198571804E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 100 0.0 18.613348 1 CGTCTAC 55 8.433326E-6 16.030636 1 CGTTAGG 70 5.2880005E-6 13.995 1 CGCATAC 45 0.009393166 13.061999 1 TAGGACC 870 0.0 12.73045 4 TTCAACG 85 2.7971528E-6 12.677822 2 AGGACCG 60 4.2180892E-4 12.621641 5 TGGGCGA 60 4.2180892E-4 12.621641 10 GGTATCA 1170 0.0 12.4758835 1 CGTATAT 60 0.0046740896 11.42925 1 CTGGGCG 75 2.1443446E-4 11.359478 9 GGCGAGG 1015 0.0 11.005322 19 AGGACCT 1525 0.0 10.6145935 5 TTAGACT 260 0.0 10.558489 4 GTCCTAT 260 0.0 10.5500765 1 GTCAACG 65 0.008476965 10.5500765 2 AGGACGT 2505 0.0 10.543228 5 GTATCAA 2295 0.0 10.458137 1 GGACGTG 2420 0.0 10.444148 6 CTAGGAC 390 0.0 10.437126 3 >>END_MODULE