FastQCFastQC Report
Thu 26 May 2016
SRR1512054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248431
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49800.22148778414814596No Hit
GTCCTACAGTGGACATTTCTAAATT46540.20698878462358863No Hit
CTGTAGGACGTGGAATATGGCAAGA42280.18804223923260266No Hit
GTCCTAAAGTGTGTATTTCTCATTT38890.17296505874540957No Hit
CTTTAGGACGTGAAATATGGCGAGG37820.16820618466833093No Hit
TATCAACGCAGAGTACTTTTTTTTT32810.14592397987752348No Hit
GGTATCAACGCAGAGTACTTTTTTT30330.13489406612878047No Hit
GTCCTACAGTGTGCATTTCTCATTT25400.11296766500728729No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC400.00529810814.2426788
TAGGACC8800.013.4999934
AGCACCG801.9911113E-613.0679935
GGTATCA12450.012.2691351
GCTCCGT550.00306820912.0914196
GGCGAGG10900.012.02187719
ATAGGAC2650.011.1178823
TGGCGAG25600.010.8674618
AGGACCT17500.010.6987835
GGACCTG16900.010.4560616
CTACACT3850.010.3679944
GTATCAA25350.010.318961
GACCGTG1303.870391E-710.22551257
CCTACAC3350.010.2132483
ACACCGT750.002647431310.133766
GCGTGCG750.002659672610.1281269
ATACGGC856.626617E-410.05365615
ATGGCGA28050.010.01980517
ACTGTTC7300.010.0153998
TATGGCG27800.010.00744516
Produced by FastQC (version 0.11.5)