FastQCFastQC Report
Thu 26 May 2016
SRR1512053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460086
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32320.13137752094845465No Hit
GTCCTACAGTGGACATTTCTAAATT28570.11613415140771502No Hit
CTGTAGGACGTGGAATATGGCAAGA28000.11381715923752259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC751.0086515E-613.8824933
GGTATCA9500.012.7963761
TAGGACC7950.012.7395094
AGTACCG854.110514E-612.2492595
GTCGAGG550.003153055712.04752619
GTCCTAG2900.011.4939651
GGCGAGG7350.011.46212619
GCGGCGC600.00603938711.0428929
GTATCAA21450.010.8320331
GTCCTAA7750.010.75241851
GGCGTGC1502.2682798E-910.7273818
ATCCCGT1605.802576E-1010.64850310
CGTTTCC1451.5290425E-810.444509514
GTCCTAC16050.010.2007081
GTAGGAC18750.010.146843
CCGGTTT1401.0227268E-710.14143212
TGGCGAG17400.010.06433318
AGGCGTG1701.6625563E-910.022127
AGGACCT14700.09.9804565
TCCAACG2600.09.82998718
Produced by FastQC (version 0.11.5)