##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512053_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2460086 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.38717833441595 31.0 30.0 34.0 25.0 34.0 2 26.44675470694927 31.0 25.0 34.0 10.0 34.0 3 28.419996292812527 31.0 28.0 34.0 16.0 34.0 4 32.918588211956816 35.0 33.0 37.0 27.0 37.0 5 33.3727528224623 35.0 35.0 37.0 28.0 37.0 6 33.65819772154307 37.0 35.0 37.0 28.0 37.0 7 33.7321463558591 37.0 35.0 37.0 29.0 37.0 8 33.775954173959775 37.0 35.0 37.0 29.0 37.0 9 35.39408988141065 39.0 37.0 39.0 30.0 39.0 10 35.30403733853207 39.0 35.0 39.0 30.0 39.0 11 35.30876562851868 39.0 35.0 39.0 30.0 39.0 12 35.20520867969656 39.0 35.0 39.0 29.0 39.0 13 35.20422863265756 39.0 35.0 39.0 29.0 39.0 14 36.39589632232369 40.0 37.0 41.0 29.0 41.0 15 36.34002591779312 40.0 37.0 41.0 29.0 41.0 16 36.28275434273436 40.0 37.0 41.0 28.0 41.0 17 36.24443738958719 40.0 37.0 41.0 28.0 41.0 18 36.187475559797505 40.0 37.0 41.0 28.0 41.0 19 36.21710948316441 40.0 37.0 41.0 28.0 41.0 20 36.11477119092584 40.0 37.0 41.0 27.0 41.0 21 36.059430036185724 40.0 37.0 41.0 27.0 41.0 22 35.955990156441686 40.0 36.0 41.0 27.0 41.0 23 35.859667507558676 40.0 36.0 41.0 27.0 41.0 24 35.76520251731037 40.0 36.0 41.0 27.0 41.0 25 35.669915604576424 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.348272567757423 1101 2 4.264719671501624 1101 3 2.411522334304422 1101 4 1.5268159524092866 1101 5 1.1602455274307104 1101 6 0.7560440779829207 1101 7 0.4790296727816994 1101 8 0.4180703707550464 1101 9 0.4919426091290475 1101 10 0.6394887348334137 1101 11 0.6328741796473523 1101 12 0.4701974633127506 1101 13 0.5832430453430888 1101 14 0.40770008031557126 1101 15 0.46965621911468247 1101 16 0.7627715844684744 1101 17 0.5885826493960593 1101 18 0.7925584670495098 1101 19 0.6047334190524296 1101 20 0.7138194055339468 1101 21 0.7148625588127615 1101 22 0.802849087604244 1101 23 0.8454838630599326 1101 24 0.8537348942153713 1101 25 0.6707724552716385 1102 1 1.5277268332934177 1102 2 4.261268095153838 1102 3 2.5607825407628226 1102 4 1.7022438235137685 1102 5 1.1270086880148966 1102 6 1.0129822204975838 1102 7 0.8263332813915341 1102 8 0.861513278657327 1102 9 0.8677086399044924 1102 10 0.6505910762577116 1102 11 0.9125760212405396 1102 12 0.7808097830946608 1102 13 0.8599761906138426 1102 14 1.0004875396360617 1102 15 1.0021614532222216 1102 16 1.1430327295980405 1102 17 1.0830080837315563 1102 18 1.227392648142036 1102 19 1.231924387123243 1102 20 1.2720582663557352 1102 21 1.184789989312307 1102 22 1.1715818569140097 1102 23 1.0578055740271992 1102 24 1.1873612801097764 1102 25 1.1173067803867767 1103 1 1.1844950123062254 1103 2 3.950395260723777 1103 3 2.2583143659383182 1103 4 1.5072897802604501 1103 5 1.0363098024986002 1103 6 0.9460005881815832 1103 7 0.692241996933852 1103 8 0.5956469202919052 1103 9 0.7091466028403772 1103 10 0.6337955863487537 1103 11 0.5611126044862189 1103 12 0.5347481707876867 1103 13 0.6264538041346341 1103 14 0.6461997842582363 1103 15 0.8491370638649371 1103 16 0.7685225832366029 1103 17 0.8105882166351037 1103 18 0.9250117954825825 1103 19 0.8526150777902046 1103 20 0.9087510401107224 1103 21 1.030160215535986 1103 22 0.9497649869039151 1103 23 1.1264048395727215 1103 24 1.2292308753221448 1103 25 1.2188324290008055 1104 1 1.3696096667380537 1104 2 3.882141085678697 1104 3 2.1648277194742427 1104 4 1.5867150448294467 1104 5 1.2680913124063693 1104 6 0.9452466727492563 1104 7 0.7135500675862829 1104 8 0.722624744642637 1104 9 0.7643442389482686 1104 10 0.719701729258496 1104 11 0.6564065333240023 1104 12 0.89586799859363 1104 13 0.7667942272562129 1104 14 0.7683220005014988 1104 15 0.7551128773968969 1104 16 0.8339635000944412 1104 17 0.6945367849887774 1104 18 0.7959193072595596 1104 19 0.892870159119795 1104 20 0.7353053652591726 1104 21 0.7561617071292517 1104 22 0.9278738732124125 1104 23 0.9823886774988395 1104 24 0.9599114383514049 1104 25 1.0384002445719105 1105 1 1.2372499834106279 1105 2 4.012805970834897 1105 3 2.3455666827442236 1105 4 1.3549490771329573 1105 5 1.0252083558261376 1105 6 0.7501205581690513 1105 7 0.5599838653848437 1105 8 0.4181740588520029 1105 9 0.4554590463906152 1105 10 0.3825266958551694 1105 11 0.42957843999467116 1105 12 0.5246504816192896 1105 13 0.4536030511866258 1105 14 0.47064723523368457 1105 15 0.2309615944366854 1105 16 0.2525355649196115 1105 17 0.5271389718837725 1105 18 0.4515911457601831 1105 19 0.4574889976840453 1105 20 0.5229307754156025 1105 21 0.5474612099423979 1105 22 0.6205989479722263 1105 23 0.5079833475103825 1105 24 0.6722556698023325 1105 25 0.7488100828725663 1106 1 0.48867105505641817 1106 2 2.3843176660285295 1106 3 1.2111520720979776 1106 4 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0.6442459823164128 1107 16 0.6015382194255778 1107 17 0.5989236231812924 1107 18 0.5289397567607139 1107 19 0.661754163688073 1107 20 0.721860841803661 1107 21 0.7595586084587893 1107 22 0.671799574076509 1107 23 0.6451550704998965 1107 24 0.8579914693812043 1107 25 0.7147387279903583 1108 1 1.3222197284502215 1108 2 3.536499867457959 1108 3 2.1478232005913185 1108 4 1.358208779729992 1108 5 0.9455041580508379 1108 6 0.7938848742088211 1108 7 0.5695266344870262 1108 8 0.6246829017896047 1108 9 0.7777817193893881 1108 10 0.7239367111704453 1108 11 0.7373934273117584 1108 12 0.6634767459492679 1108 13 0.786834534189758 1108 14 0.7418190651052541 1108 15 0.7916294570656817 1108 16 0.8086557529015295 1108 17 0.8467753219222018 1108 18 0.8170776934801012 1108 19 0.8118231802621594 1108 20 0.8852857124996518 1108 21 0.791048985409823 1108 22 0.837873748035598 1108 23 0.783209837387183 1108 24 0.8277898165606246 1108 25 0.8995270862357572 1109 1 0.877521476623297 1109 2 3.2971279836636995 1109 3 1.898138545270836 1109 4 1.1168071285837726 1109 5 0.6030920106328708 1109 6 0.4484064351879553 1109 7 0.25576319917322365 1109 8 0.24908522912968323 1109 9 0.31545425480211264 1109 10 0.20906667171641402 1109 11 0.17207802186077004 1109 12 0.16967514021860808 1109 13 0.07108511663249573 1109 14 0.10384661503939441 1109 15 0.292913217474279 1109 16 0.24004579224884282 1109 17 0.3374915152679918 1109 18 0.3711082802094481 1109 19 0.3894439205891942 1109 20 0.23378553151922432 1109 21 0.39218888046193 1109 22 0.2926780001417768 1109 23 0.35529666767438073 1109 24 0.3922754690076573 1109 25 0.47268349452878056 1110 1 0.8951977267242235 1110 2 3.603540127235096 1110 3 1.8837758157000728 1110 4 1.0868456983353951 1110 5 0.5113910286879886 1110 6 0.3775772165825586 1110 7 0.28809409161889477 1110 8 0.08781273787154475 1110 9 -0.09385093068671324 1110 10 0.02165867082204187 1110 11 -0.07661923974897888 1110 12 -0.061206125813306755 1110 13 -0.074181859337493 1110 14 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-1.7470213518125774 2315 16 -1.588543865877483 2315 17 -1.44611272521486 2315 18 -1.3543913138739825 2315 19 -1.4151916389384098 2315 20 -1.5119186146488488 2315 21 -1.4763981719709562 2315 22 -1.6971806512882779 2315 23 -1.6283161726267323 2315 24 -1.595203427321536 2315 25 -1.4517914337824607 2316 1 -0.8086452977787708 2316 2 -0.393240471410639 2316 3 -0.3585753290900975 2316 4 -0.6193684605329963 2316 5 -1.1680192310666087 2316 6 -1.0935222243877618 2316 7 -1.2532278098177798 2316 8 -1.3008354384380425 2316 9 -1.3717996677459254 2316 10 -1.56195642283609 2316 11 -1.525371587353547 2316 12 -1.467070467115235 2316 13 -1.583466214389425 2316 14 -1.8454981578347898 2316 15 -1.6462946334982789 2316 16 -1.8096691986426237 2316 17 -1.918811005740416 2316 18 -1.9116677672724833 2316 19 -1.6682630782942738 2316 20 -1.6572939772073383 2316 21 -1.7981208098477666 2316 22 -1.9171415920307595 2316 23 -1.8062476703405466 2316 24 -2.0721130836161947 2316 25 -2.040509195134497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85086.0 3 1.0 4 740.0 5 3410.0 6 3993.0 7 3602.0 8 3406.0 9 3357.0 10 3193.0 11 3103.0 12 3102.0 13 3080.0 14 3295.0 15 3351.0 16 3533.0 17 3727.0 18 4298.0 19 5212.0 20 7036.0 21 8770.0 22 10774.0 23 13055.0 24 14837.0 25 17093.0 26 19308.0 27 21943.0 28 25551.0 29 31233.0 30 38542.0 31 49650.0 32 64599.0 33 86818.0 34 120460.0 35 174922.0 36 280647.0 37 390535.0 38 944824.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.846550892936264 18.7331255899184 13.081737792906425 25.33858572423891 2 17.040963469906302 21.51471581974305 38.73419320341955 22.710127506931098 3 18.481467721047153 24.548369447246966 29.75290294729534 27.217259884410545 4 12.428589894824816 17.120092549610057 38.43133939220011 32.01997816336502 5 13.403637108621405 37.549378355065635 35.77387131994573 13.273113216367232 6 31.4445917744339 37.28300555346439 18.410697837392675 12.86170483470903 7 27.87678967320655 31.498614276086283 22.539943725544553 18.08465232516262 8 24.90380417595157 36.8520450098086 20.821751759897825 17.422399054342 9 26.119655979506405 15.902899329535632 20.46944700307225 37.507997687885705 10 14.800185684716457 29.246074524284538 34.42459688368365 21.52914290731536 11 33.907513802363006 22.75278181331872 24.724989288992337 18.614715095325934 12 22.967327158481453 24.731777669561144 31.80498567936243 20.495909492594976 13 28.60143913668059 22.002035701190934 25.948808293693798 23.447716868434682 14 21.018736743349624 22.03467683650084 27.737201057198813 29.209385362950723 15 22.883102460645684 30.52889207938259 23.60697959339633 22.981025866575397 16 22.310764745622713 28.22409460482276 27.395668281515363 22.06947236803917 17 21.807896146720076 28.457338483288797 27.68000793468196 22.05475743530917 18 21.60831616584203 27.822798970438313 29.254584004721806 21.314300858997854 19 23.35368505774226 27.14109761104359 27.48275455576467 22.022462775449476 20 23.410900168775548 27.406514931270305 27.963084007297347 21.2195008926568 21 23.145259342757967 26.854395129769955 27.14939429034145 22.850951237130626 22 23.11050281173604 27.75236723215745 27.417658043792674 21.719471912313836 23 22.606050973408447 27.51876270036548 27.527746195530444 22.347440130695627 24 22.922897817393377 27.00934032387486 28.088692834315548 21.97906902441622 25 22.886478756559246 27.253211992957944 27.73892849695355 22.12138075352926 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 170.0 2 304.5 3 439.0 4 439.0 5 439.0 6 1165.0 7 1891.0 8 1891.0 9 1891.0 10 2951.0 11 4011.0 12 4011.0 13 4011.0 14 7305.5 15 10600.0 16 10600.0 17 10600.0 18 19358.5 19 28117.0 20 28117.0 21 28117.0 22 44446.5 23 60776.0 24 60776.0 25 60776.0 26 86917.0 27 113058.0 28 113058.0 29 113058.0 30 146532.5 31 180007.0 32 180007.0 33 180007.0 34 206447.5 35 232888.0 36 232888.0 37 232888.0 38 254871.0 39 276854.0 40 276854.0 41 276854.0 42 294053.5 43 311253.0 44 311253.0 45 311253.0 46 317395.0 47 323537.0 48 323537.0 49 323537.0 50 314558.5 51 305580.0 52 305580.0 53 305580.0 54 272980.0 55 240380.0 56 240380.0 57 240380.0 58 205445.5 59 170511.0 60 170511.0 61 170511.0 62 137998.5 63 105486.0 64 105486.0 65 105486.0 66 80769.5 67 56053.0 68 56053.0 69 56053.0 70 40367.5 71 24682.0 72 24682.0 73 24682.0 74 16980.5 75 9279.0 76 9279.0 77 9279.0 78 6163.0 79 3047.0 80 3047.0 81 3047.0 82 2022.5 83 998.0 84 998.0 85 998.0 86 662.5 87 327.0 88 327.0 89 327.0 90 209.0 91 91.0 92 91.0 93 91.0 94 65.0 95 39.0 96 39.0 97 39.0 98 25.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4519524927177345 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 8.129797088394471E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.0648985441972356E-4 19 6.503837670715577E-4 20 5.69085796187613E-4 21 0.0032112698499158158 22 0.0016259594176788942 23 7.723307233974747E-4 24 0.0 25 3.658408689777512E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2460086.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.60009935164999 #Duplication Level Percentage of deduplicated Percentage of total 1 82.48463269554833 60.708771613767134 2 12.03543912921588 17.716190313020494 3 3.1250348471467655 6.900086256821107 4 1.0611441340265293 3.1240125476309255 5 0.474872015283722 1.7475313752100092 6 0.2379044836091894 1.0505876177903595 7 0.13899661140496752 0.7161115086263806 8 0.09203977381090646 0.5419309197428887 9 0.0659869074451414 0.43709786494834846 >10 0.2585449791864384 3.177030347510701 >50 0.013620566427876358 0.6916989232104207 >100 0.009610099723882881 1.4052813332226424 >500 0.0014491714468980053 0.7578953948493934 >1k 7.245857234338001E-4 1.025773983649261 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3232 0.13137752094845465 No Hit GTCCTACAGTGGACATTTCTAAATT 2857 0.11613415140771502 No Hit CTGTAGGACGTGGAATATGGCAAGA 2800 0.11381715923752259 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.0648985441972355E-5 0.0 10 0.0 0.0 0.0 4.0648985441972355E-5 0.0 11 0.0 0.0 0.0 4.0648985441972355E-5 0.0 12 0.0 0.0 0.0 4.0648985441972355E-5 0.0 13 0.0 0.0 0.0 4.0648985441972355E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACAC 75 1.0086515E-6 13.882493 3 GGTATCA 950 0.0 12.796376 1 TAGGACC 795 0.0 12.739509 4 AGTACCG 85 4.110514E-6 12.249259 5 GTCGAGG 55 0.0031530557 12.047526 19 GTCCTAG 290 0.0 11.493965 1 GGCGAGG 735 0.0 11.462126 19 GCGGCGC 60 0.006039387 11.042892 9 GTATCAA 2145 0.0 10.832033 1 GTCCTAA 775 0.0 10.7524185 1 GGCGTGC 150 2.2682798E-9 10.727381 8 ATCCCGT 160 5.802576E-10 10.648503 10 CGTTTCC 145 1.5290425E-8 10.4445095 14 GTCCTAC 1605 0.0 10.200708 1 GTAGGAC 1875 0.0 10.14684 3 CCGGTTT 140 1.0227268E-7 10.141432 12 TGGCGAG 1740 0.0 10.064333 18 AGGCGTG 170 1.6625563E-9 10.02212 7 AGGACCT 1470 0.0 9.980456 5 TCCAACG 260 0.0 9.829987 18 >>END_MODULE