FastQCFastQC Report
Thu 26 May 2016
SRR1512053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460086
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41370.16816485277343962No Hit
GTCCTACAGTGGACATTTCTAAATT33690.13694643195400485No Hit
CTGTAGGACGTGGAATATGGCAAGA31100.12641834472453403No Hit
CTTTAGGACGTGAAATATGGCGAGG27270.11084978330025862No Hit
GTCCTAAAGTGTGTATTTCTCATTT27230.11068718735849073No Hit
TATCAACGCAGAGTACTTTTTTTTT26340.10706942765415518No Hit
GGTATCAACGCAGAGTACTTTTTTT25990.10564671316368615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGC400.00527714914.2512666
GGTATCA12250.012.6992781
TAGGACC6200.011.8054384
CGGTTTC1451.2296368E-911.13167713
GGATCGT600.005873420711.0843186
CCGGTTT1201.286262E-711.07710912
ACCGATC700.001498945210.8510468
GTCCTAT2400.010.7369281
TGTAGGA18250.010.2609222
GTAGGAC16600.010.1928343
CTACACT3850.09.8760284
GCCTAAG1451.5128171E-79.8730371
AGGACGT14850.09.8577395
GGCGAGG7650.09.80654119
TAGGACG16000.09.802734
CTGTAGG18450.09.7249861
GGACGTG15150.09.7203366
TGGCGAG17750.09.6289818
GTCCTAC17150.09.516111
AATCCCG2600.09.49620919
Produced by FastQC (version 0.11.5)