FastQCFastQC Report
Thu 26 May 2016
SRR1512052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053751
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33420.16272664018179417No Hit
GTATCAACGCAGAGTACTTTTTTTT32060.15610461053944708No Hit
CTGTAGGACGTGGAATATGGCAAGA31190.15186845922412212No Hit
GTCCTAAAGTGTGTATTTCTCATTT28610.13930607946143422No Hit
CTTTAGGACGTGAAATATGGCGAGG27180.13234321005808397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6850.012.9895394
GGTATCA9050.012.6719171
GCGTGCG854.1085423E-612.2495369
AGGGCCG550.003153079712.0473585
TGTCGAG1104.0126906E-812.04735818
CGTGCGC802.9759953E-511.83193810
GTATTAA2450.011.2020371
CGAAGTG600.00603670111.04341114
GGCGAGG8650.010.72423719
GTAGGAC19700.010.6670223
TGTAGGA19550.010.5287692
GTATCAA18750.010.3506821
CGGTAGG951.19885925E-410.3176661
GTCCTAC16750.010.2991861
AGGACGT19950.010.2486665
GGACGTG19150.010.1825116
CTAGGAC3000.010.0968333
GTCCTAA10200.010.090071
TTAGGAC12600.010.0667843
TAGGACG21250.09.8889574
Produced by FastQC (version 0.11.5)