FastQCFastQC Report
Thu 26 May 2016
SRR1512052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053751
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40180.1956420228158136No Hit
GTCCTACAGTGGACATTTCTAAATT35570.17319528998403408No Hit
CTGTAGGACGTGGAATATGGCAAGA34370.16735232265255134No Hit
GTCCTAAAGTGTGTATTTCTCATTT31030.15108939691325776No Hit
CTTTAGGACGTGAAATATGGCGAGG30510.14855744440294857No Hit
TATCAACGCAGAGTACTTTTTTTTT26240.12776621898175583No Hit
GGTATCAACGCAGAGTACTTTTTTT24590.11973213890096705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10150.012.3144581
TAGGACC7500.011.5308654
GGCGAGG8300.011.21412219
TTAGGAC12100.010.9957393
AAGGCGT700.001492074510.8568656
AGGACGT20250.010.8409835
GGACGTG19550.010.6902636
TAGGACG21200.010.668984
CTAGGAC3650.010.414753
GTGGTAT4500.010.3894751
TTTAGGA13750.010.2291792
TGTAGGA19750.010.1049452
AGGACCT14050.010.07840255
TGGACCG856.572824E-410.0624875
AACTGCG856.6230545E-410.0541577
GTCCTAA11700.010.0306551
GTAGGAC19200.09.9489353
GTATCAA22250.09.9487381
GACGTGG9400.09.8996627
GACGTGA11800.09.8979497
Produced by FastQC (version 0.11.5)