##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512052_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2053751 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.261700176895836 33.0 31.0 34.0 28.0 34.0 2 31.458524913682332 33.0 31.0 34.0 28.0 34.0 3 31.572581096734705 34.0 31.0 34.0 28.0 34.0 4 35.1090059116222 37.0 35.0 37.0 32.0 37.0 5 34.797112697693144 37.0 35.0 37.0 32.0 37.0 6 34.74825770017884 37.0 35.0 37.0 32.0 37.0 7 34.63215988695806 37.0 35.0 37.0 32.0 37.0 8 34.5449988825325 37.0 35.0 37.0 31.0 37.0 9 36.118004081312684 39.0 37.0 39.0 32.0 39.0 10 35.939170327853766 39.0 37.0 39.0 31.0 39.0 11 35.94162973018638 39.0 37.0 39.0 31.0 39.0 12 35.792737288989755 39.0 35.0 39.0 30.0 39.0 13 35.7731370550763 39.0 36.0 39.0 30.0 39.0 14 36.877362932507395 40.0 37.0 41.0 31.0 41.0 15 36.8094437933323 40.0 37.0 41.0 31.0 41.0 16 36.723229349614435 40.0 37.0 41.0 30.0 41.0 17 36.64509865119968 40.0 37.0 41.0 30.0 41.0 18 36.57252997077055 40.0 36.0 41.0 30.0 41.0 19 36.59038169671007 40.0 37.0 41.0 30.0 41.0 20 36.481126485148394 40.0 36.0 41.0 30.0 41.0 21 36.36989148148923 40.0 36.0 41.0 30.0 41.0 22 36.26079743844312 40.0 36.0 41.0 30.0 41.0 23 36.36155161945143 40.0 37.0 41.0 30.0 41.0 24 36.277324758454164 40.0 37.0 41.0 30.0 41.0 25 35.82546910506678 39.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1829627481569176 1101 2 0.2626041065538196 1101 3 0.22752069967750543 1101 4 0.14148881565044036 1101 5 0.02105101340364257 1101 6 0.042230264599659506 1101 7 -0.023840987507099953 1101 8 0.0880089635154917 1101 9 -0.10988236932060857 1101 10 -0.05328992289658885 1101 11 -0.10568722412413933 1101 12 0.10079583054272234 1101 13 5.538428241607107E-4 1101 14 -0.18238853244076125 1101 15 -0.11428155105868143 1101 16 0.13208138125182955 1101 17 0.0565073519396293 1101 18 0.09891307287111317 1101 19 -0.07046065623622155 1101 20 0.01152061855589892 1101 21 0.07295356473491665 1101 22 0.16330058644505385 1101 23 -0.007237972762055733 1101 24 -0.09641093403769219 1101 25 -0.0595278843321978 1102 1 0.2871953611360638 1102 2 0.32482644787216586 1102 3 0.21907463045782194 1102 4 0.21373993768798272 1102 5 0.41058504854257905 1102 6 0.4977415898810804 1102 7 0.43622690488676596 1102 8 0.4242382331876371 1102 9 0.4920081043069473 1102 10 0.6156474254458999 1102 11 0.5995755196681642 1102 12 0.7087195365506389 1102 13 0.6571744344937116 1102 14 0.6990318256990307 1102 15 0.7978848024105076 1102 16 0.8401971107586803 1102 17 0.7915616749769754 1102 18 0.8538983860381819 1102 19 0.8305856665821878 1102 20 0.9820899385829023 1102 21 0.6559313201124866 1102 22 0.9414587116389015 1102 23 0.8878908563051056 1102 24 0.9657777689598817 1102 25 1.0665605148942348 1103 1 0.1790165893834903 1103 2 0.0765449882940672 1103 3 0.15446034125963948 1103 4 0.20253866867897585 1103 5 0.2615570554406901 1103 6 0.3072263870312568 1103 7 0.27323883287463957 1103 8 0.29548542429673574 1103 9 0.24583004675299236 1103 10 0.18192636355449565 1103 11 0.41917014663003727 1103 12 0.4030376292919442 1103 13 0.2367917938749713 1103 14 0.4958352425297292 1103 15 0.3574376443984235 1103 16 0.43607555328311065 1103 17 0.432061744654888 1103 18 0.3001854242042583 1103 19 0.5213420203369594 1103 20 0.2861055638550809 1103 21 0.3776513411900524 1103 22 0.5324733091618512 1103 23 0.44076278394459223 1103 24 0.5293218489501257 1103 25 0.6494962849222787 1104 1 0.13696677059190776 1104 2 0.16867937794833665 1104 3 0.2244227056653969 1104 4 0.28329708103527196 1104 5 0.198917924408903 1104 6 0.29207228706079746 1104 7 0.2675180769172343 1104 8 0.4268500838704057 1104 9 0.4541791569385296 1104 10 0.364060586771636 1104 11 0.41349724134524024 1104 12 0.42211288383671786 1104 13 0.320097734431954 1104 14 0.5179885436020015 1104 15 0.7739449821950402 1104 16 0.6125951242082124 1104 17 0.6322216283153423 1104 18 0.6450103739610498 1104 19 0.5453406930009095 1104 20 0.6662661548732984 1104 21 0.7321660521511859 1104 22 0.5315053732734327 1104 23 0.5053190625769801 1104 24 0.4269130877000151 1104 25 0.5496907042855739 1105 1 0.21838164641226498 1105 2 0.2961488650948283 1105 3 0.19109143456871536 1105 4 0.18180979596157698 1105 5 0.23458529718539722 1105 6 0.27802499090051924 1105 7 0.11362812116458088 1105 8 0.19612242779208344 1105 9 0.1624084710551159 1105 10 0.2577996362950117 1105 11 0.2620592024828028 1105 12 0.2650845338570065 1105 13 0.2257509025223925 1105 14 0.13869942132291868 1105 15 0.23580490518287434 1105 16 0.327786466773766 1105 17 0.04551043383487752 1105 18 0.30010451093976087 1105 19 0.32862346029152434 1105 20 0.4354704449854552 1105 21 0.5177586894713997 1105 22 0.5020519319778813 1105 23 0.07599925421650511 1105 24 0.3842426067204414 1105 25 0.07824336061135995 1106 1 -0.2617803462040662 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25 -0.3423811321537116 2315 1 -0.6647419207426637 2315 2 -0.5788512853351016 2315 3 -0.8948307309804235 2315 4 -0.7482039834198986 2315 5 -0.8530896778933723 2315 6 -0.7265266175965692 2315 7 -0.8088731875757986 2315 8 -0.7321373386203192 2315 9 -0.8939383682115078 2315 10 -0.9631456252625199 2315 11 -0.8766618623204039 2315 12 -0.9213428756794428 2315 13 -0.9448137713154381 2315 14 -1.0498953826267723 2315 15 -1.1212730235374053 2315 16 -1.1355553641790976 2315 17 -1.1295210060202265 2315 18 -1.0115720607019938 2315 19 -1.0709834161613756 2315 20 -1.0848394005530935 2315 21 -1.0231093112747303 2315 22 -1.1724353526816742 2315 23 -1.0576635540896362 2315 24 -1.2036146894319657 2315 25 -1.1415371721512741 2316 1 -0.9862113670599157 2316 2 -1.0473125248856725 2316 3 -0.9930874157920933 2316 4 -0.9432211375882105 2316 5 -1.0552318731619437 2316 6 -1.2597495752873797 2316 7 -1.2095062145807418 2316 8 -1.195012264307735 2316 9 -1.4889382519683565 2316 10 -1.45132402861298 2316 11 -1.3736485076426135 2316 12 -1.5790004764871668 2316 13 -1.3135055044344384 2316 14 -2.05093858190407 2316 15 -1.7755489449304633 2316 16 -1.8162510296379466 2316 17 -1.7686893028587463 2316 18 -1.7065078446624327 2316 19 -1.7608333960679161 2316 20 -1.7716893107137395 2316 21 -1.8689473081727002 2316 22 -1.6922800518277867 2316 23 -1.823729520418972 2316 24 -1.9184310418546389 2316 25 -2.0657951256739366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25096.0 3 1.0 4 470.0 5 3915.0 6 5235.0 7 4093.0 8 3403.0 9 3025.0 10 2975.0 11 2693.0 12 2720.0 13 2637.0 14 2605.0 15 2605.0 16 2749.0 17 2875.0 18 3037.0 19 3386.0 20 4095.0 21 5313.0 22 6820.0 23 8455.0 24 10657.0 25 12623.0 26 14661.0 27 16562.0 28 19355.0 29 23577.0 30 30353.0 31 39576.0 32 51469.0 33 68453.0 34 91674.0 35 125607.0 36 193466.0 37 374323.0 38 883192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.45058837054014 19.712064103159907 13.864265081046176 24.973082445253777 2 14.807345194232408 20.7810976111515 40.847746391845945 23.563810802770153 3 18.370094524604006 24.969239211569466 30.15647953427655 26.50418672954998 4 12.706457598803361 17.637532495419357 38.260626531648676 31.395383374128606 5 13.263534146444824 37.73579023613486 35.87528501848814 13.125390598932164 6 31.454810117825648 37.58909023946092 18.19389543692338 12.76220420579005 7 28.011088004339378 31.591268853916567 22.561547139843146 17.836096001900913 8 24.923225843834036 37.121053136431826 20.654694751213754 17.30102626852038 9 26.145233769819225 15.597216994660016 20.63453651392014 37.623012721600624 10 14.904630600301594 29.229930989686675 34.44219868913028 21.42323972088145 11 33.78208945485602 22.73776129628178 24.893621476021195 18.58652777284101 12 23.21352491124776 24.888849719367148 31.758815942146835 20.138809427238257 13 28.534033580507085 22.186818168317384 26.02744928669542 23.251698964480113 14 20.792345323264602 22.30908225972866 27.8131574859854 29.085414931021337 15 22.666585514303105 31.079829580036517 23.57200243457091 22.68158247108947 16 22.058711109574627 28.41196425467352 27.722834949319562 21.80648968643229 17 21.644396034378072 28.80178755847228 27.84397913865897 21.709837268490677 18 21.495692515791838 27.697758881188616 29.82265133407117 20.98389726894838 19 23.41194234354603 27.079865085884315 27.655543442218654 21.852649128351004 20 23.525610968039423 27.432076655910727 28.14229002667804 20.90002234937181 21 22.991979066595707 26.85851400680998 27.27886681491573 22.870640111678583 22 23.177882810525716 28.013717339638543 27.480059656696454 21.328340193139287 23 22.33157768395487 27.77522688972519 27.852840972445055 22.040354453874887 24 22.880443145099246 27.082258029959817 28.51013106203291 21.52716776290802 25 22.697954868515435 27.33907926473245 28.101578223832256 21.86138764291986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 137.0 1 137.0 2 308.0 3 479.0 4 479.0 5 479.0 6 1141.5 7 1804.0 8 1804.0 9 1804.0 10 2756.0 11 3708.0 12 3708.0 13 3708.0 14 6544.5 15 9381.0 16 9381.0 17 9381.0 18 17020.0 19 24659.0 20 24659.0 21 24659.0 22 38946.5 23 53234.0 24 53234.0 25 53234.0 26 76280.5 27 99327.0 28 99327.0 29 99327.0 30 128118.0 31 156909.0 32 156909.0 33 156909.0 34 178663.0 35 200417.0 36 200417.0 37 200417.0 38 217640.5 39 234864.0 40 234864.0 41 234864.0 42 246999.0 43 259134.0 44 259134.0 45 259134.0 46 264039.5 47 268945.0 48 268945.0 49 268945.0 50 259929.5 51 250914.0 52 250914.0 53 250914.0 54 222201.0 55 193488.0 56 193488.0 57 193488.0 58 165330.5 59 137173.0 60 137173.0 61 137173.0 62 110785.0 63 84397.0 64 84397.0 65 84397.0 66 64314.5 67 44232.0 68 44232.0 69 44232.0 70 31958.0 71 19684.0 72 19684.0 73 19684.0 74 13564.5 75 7445.0 76 7445.0 77 7445.0 78 4948.0 79 2451.0 80 2451.0 81 2451.0 82 1603.5 83 756.0 84 756.0 85 756.0 86 463.5 87 171.0 88 171.0 89 171.0 90 103.0 91 35.0 92 35.0 93 35.0 94 20.0 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4648080512194517 2 0.0 3 0.0 4 0.0 5 0.039634795065224555 6 0.0514668039114771 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 4.86913944290228E-5 16 0.0 17 0.0 18 0.0 19 0.0 20 9.73827888580456E-5 21 0.0 22 0.0 23 0.0 24 0.0032623234267445274 25 0.011685934662965471 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2053751.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.85172509693344 #Duplication Level Percentage of deduplicated Percentage of total 1 84.1672067222599 62.158934130291065 2 11.043823866702535 16.312108884453362 3 2.6115387840687063 5.785999330830657 4 0.9301949660859424 2.747860116877214 5 0.40398257743400934 1.4917405126303542 6 0.22894325049084788 1.014471239882908 7 0.14695694615128205 0.7597116791774532 8 0.0954849882493717 0.5641384882461215 9 0.06308996783501619 0.41933726648333897 >10 0.2775138376684584 3.457488739518765 >50 0.01647294396589083 0.8356423266037147 >100 0.011887038009122339 1.7901698428996877 >500 0.0017160656376022404 0.8916740462914319 >1k 0.0011880454414167313 1.7707233958139623 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4018 0.1956420228158136 No Hit GTCCTACAGTGGACATTTCTAAATT 3557 0.17319528998403408 No Hit CTGTAGGACGTGGAATATGGCAAGA 3437 0.16735232265255134 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3103 0.15108939691325776 No Hit CTTTAGGACGTGAAATATGGCGAGG 3051 0.14855744440294857 No Hit TATCAACGCAGAGTACTTTTTTTTT 2624 0.12776621898175583 No Hit GGTATCAACGCAGAGTACTTTTTTT 2459 0.11973213890096705 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.86913944290228E-5 0.0 0.0 0.0 6 0.0 4.86913944290228E-5 0.0 0.0 0.0 7 0.0 4.86913944290228E-5 0.0 4.86913944290228E-5 0.0 8 0.0 4.86913944290228E-5 0.0 4.86913944290228E-5 0.0 9 0.0 4.86913944290228E-5 0.0 1.4607418328706838E-4 0.0 10 0.0 4.86913944290228E-5 0.0 1.4607418328706838E-4 0.0 11 0.0 4.86913944290228E-5 0.0 1.947655777160912E-4 0.0 12 0.0 4.86913944290228E-5 0.0 1.947655777160912E-4 0.0 13 0.0 4.86913944290228E-5 0.0 1.947655777160912E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1015 0.0 12.314458 1 TAGGACC 750 0.0 11.530865 4 GGCGAGG 830 0.0 11.214122 19 TTAGGAC 1210 0.0 10.995739 3 AAGGCGT 70 0.0014920745 10.856865 6 AGGACGT 2025 0.0 10.840983 5 GGACGTG 1955 0.0 10.690263 6 TAGGACG 2120 0.0 10.66898 4 CTAGGAC 365 0.0 10.41475 3 GTGGTAT 450 0.0 10.389475 1 TTTAGGA 1375 0.0 10.229179 2 TGTAGGA 1975 0.0 10.104945 2 AGGACCT 1405 0.0 10.0784025 5 TGGACCG 85 6.572824E-4 10.062487 5 AACTGCG 85 6.6230545E-4 10.054157 7 GTCCTAA 1170 0.0 10.030655 1 GTAGGAC 1920 0.0 9.948935 3 GTATCAA 2225 0.0 9.948738 1 GACGTGG 940 0.0 9.899662 7 GACGTGA 1180 0.0 9.897949 7 >>END_MODULE