FastQCFastQC Report
Thu 26 May 2016
SRR1512051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030682
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47570.23425627449300285No Hit
GGTATCAACGCAGAGTACTTTTTTT28020.1379831997328976No Hit
TATCAACGCAGAGTACTTTTTTTTT26900.13246781130674326No Hit
GTCCTACAGTGGACATTTCTAAATT24390.12010743188741516No Hit
CTGTAGGACGTGGAATATGGCAAGA22840.11247452826193367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG954.9465598E-813.4050761
TCCAACG1950.013.10749618
CGATAAT450.00939579113.0613571
GACCTAC1700.012.6771991
TAGGACC7100.012.6661654
GGTATCA10600.012.2912281
AAAGACG550.003151786512.0480085
CGTTAGG751.5594804E-411.755221
GCGCCAC1302.7666829E-911.65082113
GGCGAGG5750.011.52446519
GAATTAC959.679585E-611.3427571
CCGGTTT1850.011.25721212
GCCGGTT1803.6379788E-1211.04400711
GGCCTAC1603.1832315E-1011.020521
TGTAGGA16750.010.8194822
TTAGGAC9300.010.7895363
GTAGGAC17550.010.7877973
AGGACCT12050.010.6053895
CCTCGAA1752.5102054E-1010.27794117
AGGACGT14350.010.2249155
Produced by FastQC (version 0.11.5)