FastQCFastQC Report
Thu 26 May 2016
SRR1512051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030682
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58810.28960713691262346No Hit
GGTATCAACGCAGAGTACTTTTTTT37080.1825987525373249No Hit
TATCAACGCAGAGTACTTTTTTTTT36100.17777278766443982No Hit
GTCCTACAGTGGACATTTCTAAATT27910.137441509798186No Hit
CTGTAGGACGTGGAATATGGCAAGA24630.12128930083587681No Hit
GTCCTACAGTGTGCATTTCTCATTT21160.10420144562270212No Hit
CTTTAGGACGTGAAATATGGCGAGG20840.10262562035808659No Hit
GTCCTAAAGTGTGTATTTCTCATTT20390.10040961607972101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC602.5805426E-514.24324210
TAGGACC6750.012.2488924
GGTATCA13150.012.1938771
GTCTTAG2300.011.6195271
GTGGTAT5150.011.4906011
GAACCGT752.0750116E-411.3996456
GGCGAGG6950.011.204144519
GACGTGG7600.011.1197237
AGGACCT11200.011.030795
TGTAGGA17400.010.9781242
CTGTAGG17600.010.9546121
AGGACGT14800.010.9161255
TTAGGAC9250.010.8904393
GGACGTG14600.010.86616
GTAGGAC17100.010.8372683
TCCAACG1909.094947E-1210.49501918
TAGGACG15500.010.4231394
TGGTATC5050.010.1621052
TGGCGAG15350.010.14502218
ATAAGAC2250.010.1370133
Produced by FastQC (version 0.11.5)