FastQCFastQC Report
Thu 26 May 2016
SRR1512050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192910
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40580.18505091408220126No Hit
TATCAACGCAGAGTACTTTTTTTTT23990.10939801451039942No Hit
GGTATCAACGCAGAGTACTTTTTTT22910.10447305178963114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG601.9175288E-514.6986161
GTCAACG603.1505656E-413.0654362
TAGGACC5900.011.8725424
GTCCTAA6600.011.5807271
GGTATCA10050.011.4078811
GCACCGT600.00603613411.0436056
AGATTCG600.00603613411.0436058
TCCAACG951.4156716E-510.9608218
GGCGAGG5800.010.7718419
GTATCAA21800.010.5182761
TGGCGAG12150.010.51795918
CTCAACG750.002047156410.4523492
ATGGCGA13450.09.99401617
AAGGCGT951.7051894E-49.9641556
TATGGCG13400.09.96066416
TGTAGGA15750.09.8301852
AGGACGT13050.09.7198235
GGACGTG13250.09.6445496
CGTGAAA7900.09.5857699
GTAGGAC16100.09.5835363
Produced by FastQC (version 0.11.5)