FastQCFastQC Report
Thu 26 May 2016
SRR1512050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192910
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48980.22335617968817692No Hit
TATCAACGCAGAGTACTTTTTTTTT32220.1469280545029208No Hit
GGTATCAACGCAGAGTACTTTTTTT29740.13561888084782323No Hit
GTCCTACAGTGGACATTTCTAAATT24290.11076605971061283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.012.613991
AATCCCG1406.8394E-1011.53276419
TGGCGAG14100.011.04530918
GGCGAGG6750.010.97497119
GTCCTAA7650.010.72740651
CTAGTAC1409.638643E-810.1824713
GTCCTAC15850.010.1143451
ACTGACC1606.2245817E-910.0888688
AGGACGT13000.010.0884795
GGACGTG13050.09.9731326
ATGGCGA15350.09.89749117
TATGGCG14900.09.87777116
GTTCTAA3000.09.8604821
GTAGGAC16200.09.6796333
GGCGAGA8450.09.66616719
TAGGACC6400.09.6521344
GTATCAA22750.09.6472641
TAGGACG14450.09.6022944
ACGTGAA8000.09.4954068
GCGTGCG800.00453475689.4954059
Produced by FastQC (version 0.11.5)