##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512050_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2192910 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.265463242905547 33.0 31.0 34.0 28.0 34.0 2 31.46316492696919 33.0 31.0 34.0 28.0 34.0 3 31.577931150845224 34.0 31.0 34.0 28.0 34.0 4 35.10960869347123 37.0 35.0 37.0 32.0 37.0 5 34.796716691519485 37.0 35.0 37.0 32.0 37.0 6 34.747752529743586 37.0 35.0 37.0 32.0 37.0 7 34.63366257621152 37.0 35.0 37.0 32.0 37.0 8 34.55584953326858 37.0 35.0 37.0 31.0 37.0 9 36.11868567337465 39.0 37.0 39.0 32.0 39.0 10 35.95075538895805 39.0 37.0 39.0 31.0 39.0 11 35.950030781017006 39.0 37.0 39.0 31.0 39.0 12 35.800305074079645 39.0 37.0 39.0 30.0 39.0 13 35.7796945611083 39.0 37.0 39.0 30.0 39.0 14 36.88840992106379 40.0 37.0 41.0 31.0 41.0 15 36.831774217820154 40.0 37.0 41.0 31.0 41.0 16 36.73822318289396 40.0 37.0 41.0 30.0 41.0 17 36.66821757390864 40.0 37.0 41.0 30.0 41.0 18 36.58905563839829 40.0 37.0 41.0 30.0 41.0 19 36.609434951730805 40.0 37.0 41.0 30.0 41.0 20 36.498373394256944 40.0 37.0 41.0 30.0 41.0 21 36.39705596672914 40.0 36.0 41.0 30.0 41.0 22 36.281056222097575 40.0 36.0 41.0 30.0 41.0 23 36.382873442138525 40.0 37.0 41.0 30.0 41.0 24 36.302207112923014 40.0 37.0 41.0 30.0 41.0 25 35.849053996744054 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11581776019976431 1101 2 0.10202544832284133 1101 3 0.14078272638867517 1101 4 0.037821935064634715 1101 5 0.17798781080057324 1101 6 0.24840285422052233 1101 7 0.1441391527006175 1101 8 0.1663596568908332 1101 9 -0.041028996591464306 1101 10 0.09585077241897721 1101 11 0.16085058709710864 1101 12 0.2169251348391441 1101 13 0.10301246089056804 1101 14 0.03949765085839374 1101 15 -0.13524746091371753 1101 16 0.14803900559930128 1101 17 0.07168929649753153 1101 18 0.19384202551714225 1101 19 0.16242757886533354 1101 20 0.1290651164447567 1101 21 0.21399849157982942 1101 22 0.14315170314105075 1101 23 0.0038550385165549983 1101 24 -0.10283907310423501 1101 25 -0.01769397238766146 1102 1 0.29052417471135783 1102 2 0.3183207394305718 1102 3 0.19210352495656835 1102 4 0.2229387738298314 1102 5 0.3960117701422945 1102 6 0.3803338534675902 1102 7 0.414787614570038 1102 8 0.34775169579739895 1102 9 0.6694137921729606 1102 10 0.5682804348292763 1102 11 0.6903349445703313 1102 12 0.5857451459026208 1102 13 0.5776034387789863 1102 14 0.5986329902151724 1102 15 0.595743430925431 1102 16 0.6520716044407067 1102 17 0.6409988087826051 1102 18 0.7546546532465186 1102 19 0.6884704459960531 1102 20 0.6424282020791381 1102 21 0.787605184983228 1102 22 0.9059025521092323 1102 23 0.7784948032640173 1102 24 0.7248612027143864 1102 25 0.7704270036568346 1103 1 0.4187422836676866 1103 2 0.31474620735816217 1103 3 0.42679093048332106 1103 4 0.41547716382280697 1103 5 0.5441553143873747 1103 6 0.5029959516220828 1103 7 0.3962862513774823 1103 8 0.5057350411908956 1103 9 0.6025205980431352 1103 10 0.6044875604675326 1103 11 0.665332664480907 1103 12 0.8142341804085902 1103 13 0.6681762296501077 1103 14 0.7287661428080199 1103 15 0.8228303840454032 1103 16 0.8809404995302046 1103 17 0.9895707783585692 1103 18 0.8014261160639293 1103 19 0.8425838893570159 1103 20 0.9874640654552493 1103 21 1.0557522662604342 1103 22 1.0038713975228717 1103 23 1.0230365932522716 1103 24 1.0901514361075826 1103 25 1.0759869959346275 1104 1 0.1269418476687889 1104 2 0.20305058059797076 1104 3 0.22336468673962173 1104 4 0.25753888302525496 1104 5 0.15892721539503896 1104 6 0.22838996158104408 1104 7 0.16809309081594392 1104 8 0.2842728911015371 1104 9 0.3478284978831212 1104 10 0.3032548310888572 1104 11 0.3685769617538668 1104 12 0.3301919957325836 1104 13 0.34579802448309493 1104 14 0.6435324609849786 1104 15 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-0.5363146667644187 2314 22 -0.4687197659195377 2314 23 -0.43248584066758866 2314 24 -0.5013629190050253 2314 25 -0.3999804395186928 2315 1 -0.7881391094333345 2315 2 -0.7321129201393006 2315 3 -1.0901485122269925 2315 4 -0.873290802380069 2315 5 -0.9438164436881067 2315 6 -0.9176945956884026 2315 7 -0.9522292443849949 2315 8 -1.0442668068093965 2315 9 -1.1413331209734565 2315 10 -1.1715835237038448 2315 11 -1.2303639588305515 2315 12 -1.1487559736698358 2315 13 -1.201689269528373 2315 14 -1.2985601815641914 2315 15 -1.235746745326935 2315 16 -1.528206765933362 2315 17 -1.327095832308089 2315 18 -1.305605021061048 2315 19 -1.3078511747125958 2315 20 -1.2895450188661997 2315 21 -1.3911927652507714 2315 22 -1.486743560975519 2315 23 -1.2232055261464652 2315 24 -1.3989537621492474 2315 25 -1.3457905115497795 2316 1 -1.0083901152581127 2316 2 -1.0298838923364997 2316 3 -1.010949569093622 2316 4 -0.9839195179268145 2316 5 -1.0521083430455818 2316 6 -1.160977487659821 2316 7 -1.1361279413164809 2316 8 -1.0992905262593453 2316 9 -1.4028406571531278 2316 10 -1.4437401921719868 2316 11 -1.4570096082630855 2316 12 -1.6638166234026173 2316 13 -1.513426794298681 2316 14 -1.880491665380923 2316 15 -1.658396117812373 2316 16 -1.6907186256583344 2316 17 -1.6459801174218782 2316 18 -1.7018417351666173 2316 19 -1.634558074370318 2316 20 -1.720310646681007 2316 21 -1.7549575523762186 2316 22 -1.751137063647711 2316 23 -1.8734144975029778 2316 24 -1.889186753201912 2316 25 -1.8348429345366242 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27520.0 3 2.0 4 470.0 5 4276.0 6 5558.0 7 4431.0 8 3710.0 9 3308.0 10 3267.0 11 3027.0 12 2922.0 13 2801.0 14 2731.0 15 2662.0 16 2730.0 17 3015.0 18 3258.0 19 3667.0 20 4351.0 21 5577.0 22 7282.0 23 8993.0 24 11020.0 25 13346.0 26 15516.0 27 17266.0 28 20360.0 29 25184.0 30 31979.0 31 41474.0 32 54394.0 33 72093.0 34 96681.0 35 132886.0 36 202952.0 37 396039.0 38 956162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.472909771321966 19.939091885393413 13.814147323048504 24.77385102023612 2 14.76353338714311 20.926166600544484 41.01198863610453 23.29831137620787 3 18.23180157872416 25.14841010346982 30.484698414435613 26.135089903370407 4 12.513555047858782 17.76051000725064 38.761280672713426 30.96465427217715 5 13.096270519306156 37.847630109383076 36.16051123597812 12.895588135332645 6 31.07165018067672 38.04312515968902 18.30072635690039 12.584498302733877 7 27.47404134232595 31.98293591620267 22.802714201677222 17.740308539794157 8 24.649757627992027 37.33938009311827 20.86980313829569 17.141059140594006 9 25.986976209693967 15.852223757472947 20.73413865594119 37.42666137689189 10 14.469175661563858 29.617084148460265 34.737403723819035 21.17633646615684 11 33.752228773638684 23.053112074822952 24.831388429073698 18.36327072246467 12 22.491666324655366 25.2995790980934 32.135564159039816 20.073190418211418 13 28.23358003748444 22.248747098604138 26.347319315430184 23.17035354848124 14 20.640427559726575 22.17783675572641 28.30307673365528 28.87865895089174 15 22.590712797150818 30.80222170540515 23.915345363010793 22.69172013443324 16 21.975867682668234 28.77427710211545 27.575459093168437 21.674396122047874 17 21.45049272427961 28.830047744777488 28.04018404768093 21.679275483261968 18 21.31104331687119 28.12409081996069 29.618406592153807 20.946459271014316 19 22.99688541709418 27.528991157867853 27.979762051338174 21.494361373699785 20 22.988708595485356 27.66706476836455 28.488075417698973 20.856151218451124 21 22.7773597639666 27.171110533492026 27.66939819691643 22.382131505624947 22 22.688391224446054 27.989155961712974 28.0078981809559 21.31455463288507 23 22.18276171844718 27.772868015559233 28.085922358874736 21.95844790711885 24 22.435872003487713 27.290806766199648 28.69254998424418 21.580771246068455 25 22.416431221509995 27.594993284397223 28.229657652480668 21.75891784161211 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 212.0 1 212.0 2 397.5 3 583.0 4 583.0 5 583.0 6 1468.0 7 2353.0 8 2353.0 9 2353.0 10 3785.0 11 5217.0 12 5217.0 13 5217.0 14 8722.5 15 12228.0 16 12228.0 17 12228.0 18 21585.0 19 30942.0 20 30942.0 21 30942.0 22 47146.5 23 63351.0 24 63351.0 25 63351.0 26 87422.0 27 111493.0 28 111493.0 29 111493.0 30 140437.5 31 169382.0 32 169382.0 33 169382.0 34 193117.5 35 216853.0 36 216853.0 37 216853.0 38 234307.5 39 251762.0 40 251762.0 41 251762.0 42 264977.5 43 278193.0 44 278193.0 45 278193.0 46 281113.5 47 284034.0 48 284034.0 49 284034.0 50 272998.5 51 261963.0 52 261963.0 53 261963.0 54 232740.5 55 203518.0 56 203518.0 57 203518.0 58 172666.0 59 141814.0 60 141814.0 61 141814.0 62 113839.0 63 85864.0 64 85864.0 65 85864.0 66 65148.5 67 44433.0 68 44433.0 69 44433.0 70 31605.5 71 18778.0 72 18778.0 73 18778.0 74 12830.0 75 6882.0 76 6882.0 77 6882.0 78 4573.0 79 2264.0 80 2264.0 81 2264.0 82 1441.0 83 618.0 84 618.0 85 618.0 86 381.5 87 145.0 88 145.0 89 145.0 90 83.5 91 22.0 92 22.0 93 22.0 94 13.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.468692285593116 2 0.0 3 0.0 4 0.0 5 0.04053973943299087 6 0.05244173267484757 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.368045200213415E-4 21 0.0 22 0.0 23 0.0 24 0.0031465039604908545 25 0.011628384201814028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2192910.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.48385882670847 #Duplication Level Percentage of deduplicated Percentage of total 1 81.01226878200804 57.910695848444234 2 12.742725723654733 18.21798413394403 3 3.449998507941183 7.398576188820671 4 1.2569112229234758 3.59395457668669 5 0.5770951063141351 2.062649255467197 6 0.30305043845631957 1.2997928855990193 7 0.1727947293312399 0.8646423826259559 8 0.10184019639403112 0.5823944177532149 9 0.07138295758887978 0.45924563366247595 >10 0.2839534718260646 3.4191043385481694 >50 0.0156472640672255 0.7532507770664827 >100 0.01016136035313419 1.4608982813594118 >500 0.0012127806967634266 0.6014755399610971 >1k 9.574584448119403E-4 1.3753357400613955 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4898 0.22335617968817692 No Hit TATCAACGCAGAGTACTTTTTTTTT 3222 0.1469280545029208 No Hit GGTATCAACGCAGAGTACTTTTTTT 2974 0.13561888084782323 No Hit GTCCTACAGTGGACATTTCTAAATT 2429 0.11076605971061283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.56015066737805E-5 0.0 5 0.0 0.0 0.0 4.56015066737805E-5 0.0 6 0.0 0.0 0.0 9.1203013347561E-5 0.0 7 0.0 0.0 0.0 9.1203013347561E-5 0.0 8 0.0 0.0 0.0 1.368045200213415E-4 0.0 9 0.0 0.0 0.0 3.64812053390244E-4 0.0 10 0.0 0.0 0.0 3.64812053390244E-4 0.0 11 0.0 0.0 0.0 4.56015066737805E-4 0.0 12 0.0 0.0 0.0 5.47218080085366E-4 1.368045200213415E-4 13 0.0 0.0 0.0 5.928195867591466E-4 1.82406026695122E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1165 0.0 12.61399 1 AATCCCG 140 6.8394E-10 11.532764 19 TGGCGAG 1410 0.0 11.045309 18 GGCGAGG 675 0.0 10.974971 19 GTCCTAA 765 0.0 10.7274065 1 CTAGTAC 140 9.638643E-8 10.182471 3 GTCCTAC 1585 0.0 10.114345 1 ACTGACC 160 6.2245817E-9 10.088868 8 AGGACGT 1300 0.0 10.088479 5 GGACGTG 1305 0.0 9.973132 6 ATGGCGA 1535 0.0 9.897491 17 TATGGCG 1490 0.0 9.877771 16 GTTCTAA 300 0.0 9.860482 1 GTAGGAC 1620 0.0 9.679633 3 GGCGAGA 845 0.0 9.666167 19 TAGGACC 640 0.0 9.652134 4 GTATCAA 2275 0.0 9.647264 1 TAGGACG 1445 0.0 9.602294 4 ACGTGAA 800 0.0 9.495406 8 GCGTGCG 80 0.0045347568 9.495405 9 >>END_MODULE