FastQCFastQC Report
Thu 26 May 2016
SRR1512049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880820
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36070.19177805425293223No Hit
GTCCTACAGTGGACATTTCTAAATT20760.1103773885858296No Hit
TATCAACGCAGAGTACTTTTTTTTT20690.11000521049329547No Hit
GGTATCAACGCAGAGTACTTTTTTT20640.10973936899862827No Hit
CTGTAGGACGTGGAATATGGCAAGA20210.10745313214449016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG500.001540835413.2523785
GTCAACG802.088551E-512.2479532
CGGTAGG1051.8206447E-712.1313071
TTAGACG550.003152401212.0476174
CTACGGA550.003152401212.0476174
TCCTATA2450.011.9979952
TAGGACC4800.011.8324814
GGTATCA9200.011.608931
GTCCTAA6750.011.4677131
CGACCAC600.00466696311.4314231
TCCAACG855.520584E-511.13674918
TCGAACT1053.617919E-610.81855619
GGCGAGG6400.010.64951619
GCACCGT803.8985407E-410.6492336
TTAGGAC8500.010.4682663
CTACACT3800.010.2133014
GGACCTG8650.010.1773026
TGGCGAG13000.010.12159218
AGGACCT8750.010.060995
CTGGACT2450.010.0455354
Produced by FastQC (version 0.11.5)