##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512049_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1880820 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.743894684233474 31.0 30.0 34.0 25.0 34.0 2 26.78800789017556 31.0 25.0 34.0 10.0 34.0 3 28.76860677789475 31.0 28.0 34.0 16.0 34.0 4 33.30484735381376 35.0 33.0 37.0 28.0 37.0 5 33.76936921130145 35.0 35.0 37.0 28.0 37.0 6 34.05484788549675 37.0 35.0 37.0 30.0 37.0 7 34.13298508097532 37.0 35.0 37.0 30.0 37.0 8 34.17730564328325 37.0 35.0 37.0 30.0 37.0 9 35.834658287342755 39.0 37.0 39.0 30.0 39.0 10 35.74677374762072 39.0 37.0 39.0 30.0 39.0 11 35.761952765283226 39.0 37.0 39.0 30.0 39.0 12 35.663599919184186 39.0 37.0 39.0 30.0 39.0 13 35.66178369009262 39.0 37.0 39.0 30.0 39.0 14 36.88813602577599 40.0 37.0 41.0 31.0 41.0 15 36.831960527854875 40.0 37.0 41.0 31.0 41.0 16 36.78672121734137 40.0 37.0 41.0 30.0 41.0 17 36.75418221839411 40.0 37.0 41.0 30.0 41.0 18 36.70340915132761 40.0 37.0 41.0 30.0 41.0 19 36.733059516593826 40.0 37.0 41.0 30.0 41.0 20 36.64133675737178 40.0 37.0 41.0 30.0 41.0 21 36.59398879212259 40.0 37.0 41.0 30.0 41.0 22 36.49444603949341 40.0 37.0 41.0 30.0 41.0 23 36.40754351825268 40.0 37.0 41.0 30.0 41.0 24 36.32036558522346 40.0 37.0 41.0 30.0 41.0 25 36.22537297561702 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1889199112816158 1101 2 4.22414939648035 1101 3 2.310538827475778 1101 4 1.4108805384507122 1101 5 1.0247613083706923 1101 6 0.8003465023482974 1101 7 0.49883314055907846 1101 8 0.37517920416819095 1101 9 0.48807685129170864 1101 10 0.6146543787054313 1101 11 0.5645643882706182 1101 12 0.5200253535634118 1101 13 0.588232964145547 1101 14 0.4721095469091168 1101 15 0.6485782008001664 1101 16 0.8047671690669347 1101 17 0.7307916626091355 1101 18 0.7853371803783133 1101 19 0.668493344365622 1101 20 0.7811946398101242 1101 21 0.6385435950604901 1101 22 0.7373699182807414 1101 23 0.8041668624904403 1101 24 0.6584229567824167 1101 25 0.5876264324274345 1102 1 1.2260627731487936 1102 2 3.9826376226412386 1102 3 2.228117819924183 1102 4 1.3052160990660155 1102 5 0.9144928274695943 1102 6 0.6481211219162972 1102 7 0.536320594529407 1102 8 0.560713941995445 1102 9 0.5461254083536176 1102 10 0.33284616306088566 1102 11 0.3785266517975927 1102 12 0.3818697150015069 1102 13 0.3967968385248568 1102 14 0.4952610434210314 1102 15 0.5490043110927871 1102 16 0.6282652418975658 1102 17 0.496850663258968 1102 18 0.5812781367371471 1102 19 0.3150245221794492 1102 20 0.3799879060439011 1102 21 0.47179937863548105 1102 22 0.31570261501158114 1102 23 0.4095976604923379 1102 24 0.4846638769293179 1102 25 0.35412063769122426 1103 1 1.1800012050034212 1103 2 4.088696996764032 1103 3 2.359273585847788 1103 4 1.4794860219203088 1103 5 0.9473899374901436 1103 6 0.9040645032277439 1103 7 0.6542689898277487 1103 8 0.6482783510774084 1103 9 0.6585474479557263 1103 10 0.6888924698433172 1103 11 0.7460773805034719 1103 12 0.6895129798361594 1103 13 0.7484515509949716 1103 14 0.7141135050821035 1103 15 0.9251859374442901 1103 16 0.6940346178594723 1103 17 0.7238487563952276 1103 18 0.7468264971892467 1103 19 0.872759258396627 1103 20 0.9741082481823611 1103 21 0.9405794052864067 1103 22 0.9932425994598333 1103 23 1.0876435607352946 1103 24 1.1231609069325756 1103 25 1.1219640102911086 1104 1 1.3633061172886904 1104 2 3.913107918628775 1104 3 2.17172721180944 1104 4 1.499313189714826 1104 5 1.1636809318301076 1104 6 0.7546401456767029 1104 7 0.8048370078734095 1104 8 0.7600964241365489 1104 9 0.894121983936401 1104 10 0.7553967925080656 1104 11 0.6924446622673273 1104 12 0.814494306246786 1104 13 0.8448537624438472 1104 14 0.7883658951465762 1104 15 0.7156583467123383 1104 16 0.8993359336579161 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-0.8176361099259353 2315 8 -0.7891552311738224 2315 9 -0.8074420443072157 2315 10 -0.7243211583856777 2315 11 -0.7892530115396426 2315 12 -0.8369796804571408 2315 13 -0.8439506179070975 2315 14 -0.9883149588313103 2315 15 -1.1491793527300445 2315 16 -1.091092221430884 2315 17 -0.8648791954790482 2315 18 -0.9460588592308525 2315 19 -0.8620646389532283 2315 20 -0.82768322139151 2315 21 -0.9033200186053989 2315 22 -1.1738893550880931 2315 23 -1.1513309646248615 2315 24 -0.9627450285938224 2315 25 -1.0133012859410329 2316 1 -0.2695619724759517 2316 2 0.24100070821951292 2316 3 0.2166857452009694 2316 4 -0.14765943608799859 2316 5 -0.3076423275769571 2316 6 -0.49587948600836285 2316 7 -0.6748142153395804 2316 8 -0.769924430415081 2316 9 -0.7388673532214227 2316 10 -0.889476521287115 2316 11 -0.9488518534290122 2316 12 -1.0146785093948196 2316 13 -1.0989636597672856 2316 14 -1.040555765180585 2316 15 -1.0073866256778956 2316 16 -1.0211690790557384 2316 17 -1.2028232212250387 2316 18 -1.0996855784037862 2316 19 -1.0874285568559117 2316 20 -0.9412371651811711 2316 21 -1.0725641809179507 2316 22 -1.088095883723625 2316 23 -0.9763105944032588 2316 24 -0.9629487308098703 2316 25 -0.9963968710541309 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52924.0 3 0.0 4 429.0 5 2083.0 6 2470.0 7 2246.0 8 2135.0 9 2157.0 10 2103.0 11 2027.0 12 1933.0 13 1959.0 14 2052.0 15 2124.0 16 2246.0 17 2507.0 18 2766.0 19 3485.0 20 4471.0 21 5656.0 22 7081.0 23 8161.0 24 9844.0 25 11267.0 26 13008.0 27 15028.0 28 17501.0 29 21683.0 30 27342.0 31 35465.0 32 47311.0 33 64575.0 34 90840.0 35 134751.0 36 219144.0 37 308028.0 38 752018.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42644165842558 19.15797364979105 13.114120436830746 25.301464254952627 2 16.985355345719842 21.682663016647982 38.93164305326925 22.400338584362927 3 18.29148988207271 24.854531534118095 29.919024680724366 26.934953903084825 4 12.172669367616253 17.17729500962346 38.78430684488681 31.865728777873482 5 13.147510128560945 37.58493635754618 36.09149200880467 13.176061505088207 6 31.076780912995883 37.761156177176005 18.452812312083193 12.70925059774492 7 27.557980029986922 31.744770897799896 22.841473399900046 17.85577567231314 8 24.837198668665796 37.021830903542075 20.87504386384662 17.265926563945513 9 26.12084090981593 15.784445082464032 20.623026126902097 37.47168788081794 10 14.540216012394607 29.31160803437653 34.81447965725552 21.333696295973347 11 33.77080209695771 22.93191267638583 24.793866505035037 18.50341872162142 12 22.799576780340487 24.96650397167193 32.002902989121765 20.231016258865814 13 28.396071926074796 22.224614795674228 26.13392031135356 23.245392966897416 14 20.798322008485663 22.10317840091024 28.07116045129252 29.027339139311575 15 22.700736912623217 30.672313140013397 23.828755542795165 22.79819440456822 16 22.04836188470986 28.490179815186995 27.566699631012003 21.89475866909114 17 21.586967386565433 28.661434906051614 27.93175317148903 21.819844535893917 18 21.41589833758061 27.828922917908372 29.60607992394778 21.149098820563236 19 23.03861701624253 27.287706367971552 27.935036258449188 21.738640357336735 20 23.152295796432064 27.39956837676593 28.405466376932882 21.042669449869127 21 22.80803803572815 27.004537532207756 27.543043815312764 22.64438061675133 22 22.834512537025685 27.906266997520724 27.79269763912911 21.466522826324482 23 22.31886554597275 27.664944526235924 27.935893202835604 22.080296724955723 24 22.603013579183546 27.144596505779393 28.55547048627726 21.696919428759795 25 22.523871376625696 27.419849649804952 28.141507191852018 21.914771781717334 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 191.0 2 357.5 3 524.0 4 524.0 5 524.0 6 1245.0 7 1966.0 8 1966.0 9 1966.0 10 3125.0 11 4284.0 12 4284.0 13 4284.0 14 7133.0 15 9982.0 16 9982.0 17 9982.0 18 17541.5 19 25101.0 20 25101.0 21 25101.0 22 38263.0 23 51425.0 24 51425.0 25 51425.0 26 71990.0 27 92555.0 28 92555.0 29 92555.0 30 117310.0 31 142065.0 32 142065.0 33 142065.0 34 162095.5 35 182126.0 36 182126.0 37 182126.0 38 197509.5 39 212893.0 40 212893.0 41 212893.0 42 223652.5 43 234412.0 44 234412.0 45 234412.0 46 239010.0 47 243608.0 48 243608.0 49 243608.0 50 235733.5 51 227859.0 52 227859.0 53 227859.0 54 202733.0 55 177607.0 56 177607.0 57 177607.0 58 151795.0 59 125983.0 60 125983.0 61 125983.0 62 102078.5 63 78174.0 64 78174.0 65 78174.0 66 59883.5 67 41593.0 68 41593.0 69 41593.0 70 29946.0 71 18299.0 72 18299.0 73 18299.0 74 12559.0 75 6819.0 76 6819.0 77 6819.0 78 4528.0 79 2237.0 80 2237.0 81 2237.0 82 1502.5 83 768.0 84 768.0 85 768.0 86 510.0 87 252.0 88 252.0 89 252.0 90 156.5 91 61.0 92 61.0 93 61.0 94 43.0 95 25.0 96 25.0 97 25.0 98 18.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.39442370880786 3 0.0 4 0.0 5 0.0 6 5.3168298933443926E-5 7 0.0 8 0.0 9 0.0 10 1.0633659786688785E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.7217809253410747E-4 19 9.038610818685467E-4 20 6.911878861347709E-4 21 0.0032964345338735235 22 0.0016482172669367618 23 0.0011165342776023224 24 0.0 25 5.848512882678831E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1880820.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.64654005197369 #Duplication Level Percentage of deduplicated Percentage of total 1 84.90706412302434 65.07832691000884 2 10.764955129088975 16.501931289188356 3 2.4540747111907044 5.642890069254423 4 0.8193353392483412 2.5119687558278176 5 0.3637550154614836 1.3940281680837456 6 0.19662104422393123 0.9042193644702262 7 0.1111257094943237 0.5962180800492471 8 0.07545187110858195 0.4626499888736227 9 0.054232499402243464 0.37410600937974237 >10 0.23120569971141725 2.9852468560080636 >50 0.012443508145425873 0.6799921469774656 >100 0.008124957568441527 1.3409368996097297 >500 9.802388106631707E-4 0.5816283399524916 >1k 6.301535211406097E-4 0.9458571223161716 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3607 0.19177805425293223 No Hit GTCCTACAGTGGACATTTCTAAATT 2076 0.1103773885858296 No Hit TATCAACGCAGAGTACTTTTTTTTT 2069 0.11000521049329547 No Hit GGTATCAACGCAGAGTACTTTTTTT 2064 0.10973936899862827 No Hit CTGTAGGACGTGGAATATGGCAAGA 2021 0.10745313214449016 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0633659786688785E-4 2 0.0 0.0 0.0 0.0 1.0633659786688785E-4 3 0.0 0.0 0.0 0.0 1.0633659786688785E-4 4 0.0 0.0 0.0 0.0 1.0633659786688785E-4 5 0.0 5.3168298933443926E-5 0.0 0.0 1.0633659786688785E-4 6 1.0633659786688785E-4 5.3168298933443926E-5 0.0 0.0 1.0633659786688785E-4 7 1.0633659786688785E-4 5.3168298933443926E-5 0.0 0.0 1.0633659786688785E-4 8 1.0633659786688785E-4 5.3168298933443926E-5 0.0 5.3168298933443926E-5 1.0633659786688785E-4 9 1.0633659786688785E-4 5.3168298933443926E-5 0.0 5.3168298933443926E-5 1.0633659786688785E-4 10 1.0633659786688785E-4 5.3168298933443926E-5 0.0 1.0633659786688785E-4 1.0633659786688785E-4 11 1.0633659786688785E-4 5.3168298933443926E-5 0.0 1.0633659786688785E-4 1.0633659786688785E-4 12 1.0633659786688785E-4 5.3168298933443926E-5 0.0 1.0633659786688785E-4 1.0633659786688785E-4 13 1.0633659786688785E-4 5.3168298933443926E-5 0.0 1.0633659786688785E-4 1.0633659786688785E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACCG 50 0.0015408354 13.252378 5 GTCAACG 80 2.088551E-5 12.247953 2 CGGTAGG 105 1.8206447E-7 12.131307 1 TTAGACG 55 0.0031524012 12.047617 4 CTACGGA 55 0.0031524012 12.047617 4 TCCTATA 245 0.0 11.997995 2 TAGGACC 480 0.0 11.832481 4 GGTATCA 920 0.0 11.60893 1 GTCCTAA 675 0.0 11.467713 1 CGACCAC 60 0.004666963 11.431423 1 TCCAACG 85 5.520584E-5 11.136749 18 TCGAACT 105 3.617919E-6 10.818556 19 GGCGAGG 640 0.0 10.649516 19 GCACCGT 80 3.8985407E-4 10.649233 6 TTAGGAC 850 0.0 10.468266 3 CTACACT 380 0.0 10.213301 4 GGACCTG 865 0.0 10.177302 6 TGGCGAG 1300 0.0 10.121592 18 AGGACCT 875 0.0 10.06099 5 CTGGACT 245 0.0 10.045535 4 >>END_MODULE