FastQCFastQC Report
Thu 26 May 2016
SRR1512049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880820
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45030.239416850097298No Hit
TATCAACGCAGAGTACTTTTTTTTT29040.15440074010272115No Hit
GGTATCAACGCAGAGTACTTTTTTT27480.1461064854691039No Hit
GTCCTACAGTGGACATTTCTAAATT23900.12707223445093097No Hit
CTGTAGGACGTGGAATATGGCAAGA22600.12016035558958327No Hit
GTCCTAAAGTGTGTATTTCTCATTT19730.10490105379568485No Hit
CTTTAGGACGTGAAATATGGCGAGG19020.10112610457141033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC604.1100208E-412.6603398
GGTATCA9900.012.6282141
GGACGTG12700.011.7447846
GGGGTTA658.020835E-411.6929966
AGGACGT13050.011.65253455
TACACCG600.005857600411.0881155
GACGTGA7500.011.0144967
AATGTCC4450.010.8822028
TTAGGAC8800.010.692113
CTAGTAG803.7564675E-410.6921093
GACGTGG6150.010.6532137
GGCGAGG7350.010.59475319
TAGGACG14900.010.5884584
TGGTATC3100.010.4238492
GTAGGAC15050.010.4197743
TAGGACC5900.010.309534
GTGGTAT3550.010.2155331
TAATACT3450.010.19280054
TAGGACT3300.010.0801044
CGTGGAA6500.010.0795779
Produced by FastQC (version 0.11.5)