FastQCFastQC Report
Thu 26 May 2016
SRR1512048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154832
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA31920.2764038405586267No Hit
GTCCTAAAGTGTGTATTTCTCATTT31440.2722473918284218No Hit
GTCCTACAGTGGACATTTCTAAATT31270.2707753162364742No Hit
CTTTAGGACGTGAAATATGGCGAGG29870.25865234077337657No Hit
GTATCAACGCAGAGTACTTTTTTTT28210.2442779555814179No Hit
TATCAACGCAGAGTACTTTTTTTTT17210.149026005514222No Hit
GGTATCAACGCAGAGTACTTTTTTT17000.14720755919475734No Hit
GTCCTACAGTGTGCATTTCTCATTT16230.1405399226900536No Hit
CTGAAGGACCTGGAATATGGCGAGA15010.1299756155007828No Hit
CTGTAGGACCTGGAATATGGCGAGA13270.11490848885378999No Hit
ATTTAGAAATGTCCACTGTAGGACG12060.10443077434639844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGG601.5192072E-615.7792845
ACGGGGA456.9380045E-414.7273318
GGACGGG653.4920267E-614.5654936
GTCTTAG1101.1641532E-1014.2325731
CGGGGAA500.001538266313.2545999
TAGGACC5900.012.9978494
GGTATCA4850.012.7102731
GTAAGAC1302.0918378E-1012.3806693
AAGGCGT658.254054E-411.6523946
GTCTAAG853.9356833E-511.5116391
GCCCTAC600.004713163211.4157081
CCTACAC1604.9112714E-1111.2427413
AATCCCG855.5084034E-511.13831819
GGCGTGC600.00602551111.0454998
GTCCTAA10550.011.0369881
GTCCTAG1708.0035534E-1110.9360561
AGGACCT12450.010.7223085
GTATCAA11050.010.6261271
GGACCTG11900.010.5814036
AATGTCC4750.010.5638148
Produced by FastQC (version 0.11.5)