##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512048_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1154832 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.403951397259515 31.0 30.0 34.0 25.0 34.0 2 27.46054317857489 31.0 27.0 34.0 10.0 34.0 3 29.446086530335148 31.0 28.0 34.0 16.0 34.0 4 33.99713205037616 37.0 35.0 37.0 30.0 37.0 5 34.47658880252712 37.0 35.0 37.0 32.0 37.0 6 34.78146951244857 37.0 35.0 37.0 32.0 37.0 7 34.8811792537789 37.0 35.0 37.0 32.0 37.0 8 34.92687074829932 37.0 35.0 37.0 32.0 37.0 9 36.65898849356443 39.0 37.0 39.0 33.0 39.0 10 36.58160061376893 39.0 37.0 39.0 33.0 39.0 11 36.600724607561965 39.0 37.0 39.0 33.0 39.0 12 36.53533500976766 39.0 37.0 39.0 33.0 39.0 13 36.54032534602435 39.0 37.0 39.0 33.0 39.0 14 37.85759400501545 40.0 38.0 41.0 33.0 41.0 15 37.82349813652549 40.0 38.0 41.0 33.0 41.0 16 37.78962481122795 40.0 38.0 41.0 33.0 41.0 17 37.77737714230295 40.0 38.0 41.0 33.0 41.0 18 37.74051030799285 40.0 38.0 41.0 33.0 41.0 19 37.784462155534314 40.0 38.0 41.0 33.0 41.0 20 37.71549281627111 40.0 38.0 41.0 33.0 41.0 21 37.66463087271568 40.0 38.0 41.0 33.0 41.0 22 37.591492961746816 40.0 38.0 41.0 33.0 41.0 23 37.512999293403716 40.0 38.0 41.0 33.0 41.0 24 37.47052211923466 40.0 38.0 41.0 33.0 41.0 25 37.396521745154274 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1165371445160233 1101 2 4.141268038653514 1101 3 2.3315283061835466 1101 4 1.2865390794610505 1101 5 0.8564917224622519 1101 6 0.5902789823815553 1101 7 0.3258165950507603 1101 8 0.14139420059717622 1101 9 0.4918697906113607 1101 10 0.4837582557684641 1101 11 0.440992628148436 1101 12 0.4065962269121144 1101 13 0.45201420233657075 1101 14 0.2322826655870429 1101 15 0.3071187635456951 1101 16 0.4974321523662226 1101 17 0.4604940230092822 1101 18 0.3701329570628502 1101 19 0.45136935116536847 1101 20 0.40755669785487925 1101 21 0.38260159959293105 1101 22 0.3696433054787178 1101 23 0.6198928229667544 1101 24 0.5551625561729523 1101 25 0.3534435689468296 1102 1 1.392416534088575 1102 2 4.107777908762614 1102 3 2.365065016995988 1102 4 1.4339273260076553 1102 5 0.9645128795168176 1102 6 0.7672274817383524 1102 7 0.5691894885256303 1102 8 0.4557517604090222 1102 9 0.5647013904874569 1102 10 0.40996087811907245 1102 11 0.4504485552213495 1102 12 0.5193655325691324 1102 13 0.5385499093301505 1102 14 0.6838505405886934 1102 15 0.602548942237874 1102 16 0.469804231451306 1102 17 0.5910976108271271 1102 18 0.6827802207054319 1102 19 0.5922339396501002 1102 20 0.6282508933286124 1102 21 0.5992385968004044 1102 22 0.545851944531698 1102 23 0.6826163628683588 1102 24 0.653512737566821 1102 25 0.8107579769693061 1103 1 1.3500211829018625 1103 2 4.180132362066811 1103 3 2.50561977637949 1103 4 1.598852433865062 1103 5 1.2872431967092695 1103 6 1.0565278024106775 1103 7 0.8902030675206518 1103 8 0.7066252838008893 1103 9 0.8102742788957329 1103 10 0.6212004992991638 1103 11 0.6263835376910123 1103 12 0.5880178481825453 1103 13 0.6146311962102757 1103 14 0.7485140965716468 1103 15 0.9281507173481671 1103 16 0.7200019856224813 1103 17 0.8486090888010196 1103 18 0.7502284248281512 1103 19 0.6869920509387413 1103 20 0.7775038428030712 1103 21 0.8644424688158452 1103 22 0.8356903361560484 1103 23 1.0599373083465764 1103 24 1.051869338664666 1103 25 1.0405202603658168 1104 1 1.4269785878798231 1104 2 4.073812337936534 1104 3 2.366070474667474 1104 4 1.5847732909258951 1104 5 1.1591383317697534 1104 6 0.9291046904047775 1104 7 0.7417996994576512 1104 8 0.7125539177875098 1104 9 0.7828377208031867 1104 10 0.7843705777588923 1104 11 0.6537030784889808 1104 12 0.7641354178568989 1104 13 0.7040207802174265 1104 14 0.83350353957659 1104 15 0.7604700219310985 1104 16 0.7352258629650947 1104 17 0.8119099940497208 1104 18 0.9096888370052127 1104 19 0.8348423124106148 1104 20 0.687048020465852 1104 21 0.7800046787710428 1104 22 0.8130689307129302 1104 23 0.9701167227513352 1104 24 0.8418138463016831 1104 25 0.8384597384396102 1105 1 1.2706607262728582 1105 2 4.205684900678822 1105 3 2.417296807707043 1105 4 1.436761546007773 1105 5 1.0104994520010848 1105 6 0.9738134291077074 1105 7 0.6517406868602365 1105 8 0.5094536683736024 1105 9 0.42754530747884445 1105 10 0.5282871537034168 1105 11 0.5527710884194548 1105 12 0.5409272522875384 1105 13 0.5732440834252515 1105 14 0.5974776304830627 1105 15 0.48659353653592774 1105 16 0.44320422789277814 1105 17 0.6886517152936804 1105 18 0.48383547279755135 1105 19 0.6614139656273608 1105 20 0.5033695109587484 1105 21 0.7266910163035121 1105 22 0.8436689353210411 1105 23 0.7161947898799639 1105 24 0.8269102133836554 1105 25 0.8427647203815809 1106 1 0.279314959174247 1106 2 2.053786609228567 1106 3 1.0617671922618932 1106 4 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16 0.6500377150564915 1107 17 0.5900293517754065 1107 18 0.6305341019979736 1107 19 0.4931961759989463 1107 20 0.5503775101383184 1107 21 0.5656092469123379 1107 22 0.6378908954618794 1107 23 0.6374464563757058 1107 24 0.6769640540153858 1107 25 0.7423433960729611 1108 1 1.1941151206250602 1108 2 3.4951689562657933 1108 3 2.1238502099243526 1108 4 1.3346671818137352 1108 5 0.9438559180131776 1108 6 0.784558130010744 1108 7 0.653486347341051 1108 8 0.7114555018525834 1108 9 0.6347669870826707 1108 10 0.5495328056165718 1108 11 0.6196073947303944 1108 12 0.5071963795858068 1108 13 0.6583337868129036 1108 14 0.4734136472071384 1108 15 0.8017441559776586 1108 16 0.6838697636284863 1108 17 0.7580925898802846 1108 18 0.5399215092272343 1108 19 0.7046185270489076 1108 20 0.6853787126242992 1108 21 0.6535510550293324 1108 22 0.7031014992786098 1108 23 0.8678701955586092 1108 24 0.8603305084538277 1108 25 0.9525442745206192 1109 1 0.979982827206257 1109 2 3.126820796897775 1109 3 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11 -0.3579152255798306 2315 12 -0.42299482970315694 2315 13 -0.43779355531445674 2315 14 -0.4708013258363053 2315 15 -0.8254253499660393 2315 16 -0.6464171469729578 2315 17 -0.6850783688452395 2315 18 -0.7781196381942124 2315 19 -0.6744815768001402 2315 20 -0.6007468066209185 2315 21 -0.7348982246560283 2315 22 -0.9993482057459175 2315 23 -0.746753876428663 2315 24 -0.44627274400983197 2315 25 -0.5122299090491396 2316 1 -0.39591113403215417 2316 2 0.34760856551353925 2316 3 0.04851172935322978 2316 4 -0.0749054707131549 2316 5 -0.6165031500689011 2316 6 -0.5488263300549576 2316 7 -0.6610546714202954 2316 8 -0.9088334577976198 2316 9 -0.8221979860649924 2316 10 -0.9192849283026305 2316 11 -1.0359093237110883 2316 12 -0.9223812271934548 2316 13 -1.0210051085328402 2316 14 -1.1730997256738078 2316 15 -1.0424770110656212 2316 16 -1.1380608681971154 2316 17 -1.1956845234900015 2316 18 -1.3299429545253503 2316 19 -1.157314812333226 2316 20 -0.9098797510101875 2316 21 -1.0639838299643287 2316 22 -1.2341379164076471 2316 23 -1.336775712214525 2316 24 -1.162971759988885 2316 25 -1.141653489440941 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22796.0 3 4.0 4 185.0 5 903.0 6 1038.0 7 996.0 8 877.0 9 847.0 10 841.0 11 810.0 12 834.0 13 745.0 14 855.0 15 867.0 16 908.0 17 979.0 18 1104.0 19 1400.0 20 1659.0 21 2297.0 22 2838.0 23 3172.0 24 3810.0 25 4451.0 26 4966.0 27 6035.0 28 7151.0 29 9018.0 30 11695.0 31 15413.0 32 21884.0 33 31719.0 34 48360.0 35 77331.0 36 137049.0 37 200059.0 38 528936.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.28828435651246 20.496747578868614 13.701906424484253 25.513061640134673 2 15.556555950940451 22.202363478846113 41.37994300729593 20.861137562917513 3 17.426777228202887 26.056690496972717 31.017758427199798 25.49877384762459 4 11.256615680895576 18.344399878077507 40.78705820413705 29.611926236889868 5 11.749847596879892 38.744077060559455 37.42596325699323 12.080112085567425 6 28.671579366660026 39.49005481326256 19.72619346570491 12.112172354372506 7 25.819080177896005 32.443680119705725 24.485033320863987 17.252206381534283 8 22.694989401055736 39.30095459772504 22.133522451750558 15.870533549468668 9 25.349834434792246 15.979986699364062 22.328355985978913 36.34182287986478 10 14.09798135140003 29.826676087950453 36.25540338334927 19.819939177300245 11 31.6196641589426 23.03252767502113 27.54435277165856 17.80345539437771 12 22.60129611926237 25.333901381326463 33.855227427019685 18.20957507239148 13 27.733298003519124 23.575636975767907 27.06575501891184 21.62531000180113 14 18.92742840517062 24.332110644665196 29.42739723180515 27.313063718359032 15 20.75687199523394 34.16401693060116 24.356183410227636 20.722927663937266 16 19.510110561536223 29.418997741662857 31.091102428751544 19.979789268049377 17 19.588130567909445 30.47984468736578 30.05857129002314 19.873453454701636 18 19.244923901824002 28.34137496276489 33.368461341295294 19.045239794115815 19 22.058483522554578 27.741442180216673 29.40282519974957 20.797249097479174 20 22.03461306657984 28.82118833692407 30.31379673439416 18.83040186210193 21 20.792719677446172 27.930504752403913 28.932337129714302 22.344438440435614 22 21.35593102922737 30.364961101758908 29.20381569923312 19.075292169780607 23 19.79177671286731 29.99087302068378 30.116780306507174 20.100569959941737 24 21.463938415177992 28.00282292631816 31.150905328056943 19.382333330446905 25 20.664689455112246 29.10233152209209 30.39014569307038 19.84283332972528 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 176.0 1 176.0 2 299.0 3 422.0 4 422.0 5 422.0 6 1062.5 7 1703.0 8 1703.0 9 1703.0 10 2467.5 11 3232.0 12 3232.0 13 3232.0 14 5555.0 15 7878.0 16 7878.0 17 7878.0 18 13668.0 19 19458.0 20 19458.0 21 19458.0 22 30796.5 23 42135.0 24 42135.0 25 42135.0 26 59717.5 27 77300.0 28 77300.0 29 77300.0 30 98441.0 31 119582.0 32 119582.0 33 119582.0 34 131268.5 35 142955.0 36 142955.0 37 142955.0 38 147169.5 39 151384.0 40 151384.0 41 151384.0 42 151918.5 43 152453.0 44 152453.0 45 152453.0 46 148689.0 47 144925.0 48 144925.0 49 144925.0 50 132016.5 51 119108.0 52 119108.0 53 119108.0 54 98498.0 55 77888.0 56 77888.0 57 77888.0 58 63098.5 59 48309.0 60 48309.0 61 48309.0 62 37198.0 63 26087.0 64 26087.0 65 26087.0 66 19263.0 67 12439.0 68 12439.0 69 12439.0 70 8677.5 71 4916.0 72 4916.0 73 4916.0 74 3290.0 75 1664.0 76 1664.0 77 1664.0 78 1104.5 79 545.0 80 545.0 81 545.0 82 359.5 83 174.0 84 174.0 85 174.0 86 116.0 87 58.0 88 58.0 89 58.0 90 42.5 91 27.0 92 27.0 93 27.0 94 17.0 95 7.0 96 7.0 97 7.0 98 7.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.246879199745071 3 0.0 4 0.0 5 0.0 6 1.7318536375853804E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 6.927414550341521E-4 19 9.525195006719592E-4 20 6.927414550341521E-4 21 0.004156448730204912 22 0.002078224365102456 23 0.0012122975463097663 24 1.7318536375853804E-4 25 6.061487731548831E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1154832.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.13128081280439 #Duplication Level Percentage of deduplicated Percentage of total 1 90.80647289880719 71.85632506575823 2 6.938916459904997 10.981706938506656 3 1.1600655152833141 2.7539241015340368 4 0.3731778311398625 1.1812015899616704 5 0.17358293050882523 0.686791980920168 6 0.1072073485921256 0.5090072883983822 7 0.07446912633486567 0.4124986143521923 8 0.05378051183184424 0.34045766272176126 9 0.04245989444054922 0.3023915247231419 >10 0.22642026580332328 3.289403396983772 >50 0.021629865741398777 1.1803600724866936 >100 0.017608938822379815 2.708485256729399 >500 0.0026579449195880283 1.3687878932808162 >1k 0.0015504678697596834 2.42865861364308 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 3192 0.2764038405586267 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3144 0.2722473918284218 No Hit GTCCTACAGTGGACATTTCTAAATT 3127 0.2707753162364742 No Hit CTTTAGGACGTGAAATATGGCGAGG 2987 0.25865234077337657 No Hit GTATCAACGCAGAGTACTTTTTTTT 2821 0.2442779555814179 No Hit TATCAACGCAGAGTACTTTTTTTTT 1721 0.149026005514222 No Hit GGTATCAACGCAGAGTACTTTTTTT 1700 0.14720755919475734 No Hit GTCCTACAGTGTGCATTTCTCATTT 1623 0.1405399226900536 No Hit CTGAAGGACCTGGAATATGGCGAGA 1501 0.1299756155007828 No Hit CTGTAGGACCTGGAATATGGCGAGA 1327 0.11490848885378999 No Hit ATTTAGAAATGTCCACTGTAGGACG 1206 0.10443077434639844 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 8.659268187926902E-5 0.0 0.0 0.0 5 0.0 1.7318536375853804E-4 0.0 0.0 0.0 6 0.0 1.7318536375853804E-4 0.0 0.0 0.0 7 0.0 2.5977804563780707E-4 0.0 0.0 0.0 8 0.0 2.5977804563780707E-4 0.0 0.0 0.0 9 0.0 3.463707275170761E-4 0.0 0.0 0.0 10 0.0 3.463707275170761E-4 0.0 0.0 0.0 11 0.0 3.463707275170761E-4 0.0 0.0 0.0 12 0.0 3.463707275170761E-4 0.0 0.0 0.0 13 0.0 3.463707275170761E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACGG 60 1.5192072E-6 15.779284 5 ACGGGGA 45 6.9380045E-4 14.727331 8 GGACGGG 65 3.4920267E-6 14.565493 6 GTCTTAG 110 1.1641532E-10 14.232573 1 CGGGGAA 50 0.0015382663 13.254599 9 TAGGACC 590 0.0 12.997849 4 GGTATCA 485 0.0 12.710273 1 GTAAGAC 130 2.0918378E-10 12.380669 3 AAGGCGT 65 8.254054E-4 11.652394 6 GTCTAAG 85 3.9356833E-5 11.511639 1 GCCCTAC 60 0.0047131632 11.415708 1 CCTACAC 160 4.9112714E-11 11.242741 3 AATCCCG 85 5.5084034E-5 11.138318 19 GGCGTGC 60 0.006025511 11.045499 8 GTCCTAA 1055 0.0 11.036988 1 GTCCTAG 170 8.0035534E-11 10.936056 1 AGGACCT 1245 0.0 10.722308 5 GTATCAA 1105 0.0 10.626127 1 GGACCTG 1190 0.0 10.581403 6 AATGTCC 475 0.0 10.563814 8 >>END_MODULE