FastQCFastQC Report
Thu 26 May 2016
SRR1512048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154832
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34960.3027280158499245No Hit
CTTTAGGACGTGAAATATGGCGAGG31350.27146805769150834No Hit
CTGTAGGACGTGGAATATGGCAAGA31000.26843731382573394No Hit
GTCCTACAGTGGACATTTCTAAATT30850.2671384235975449No Hit
GTCCTAAAGTGTGTATTTCTCATTT30780.26653227482439No Hit
TATCAACGCAGAGTACTTTTTTTTT23940.20730288041897002No Hit
GGTATCAACGCAGAGTACTTTTTTT22690.19647879518406142No Hit
GTCCTACAGTGTGCATTTCTCATTT16240.14062651537193288No Hit
CTGAAGGACCTGGAATATGGCGAGA15930.13794214223367554No Hit
TTTCTAAATTTTCCACCTTTTTCAG13930.12062360585782174No Hit
ATTTAGAAATGTCCACTGTAGGACG13920.12053701317594247No Hit
CTGTAGGACCTGGAATATGGCGAGA13500.11690012053701318No Hit
GAATATGGCAAGAAAACTGAAAATC12250.10607603530210455No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12040.1042575889826399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG555.9341073E-717.26501316
GTTATGC350.002164299116.2918423
GTCGAGG601.4708658E-615.82831719
CCAACGA456.776726E-414.77309619
ATGTCGA653.3859706E-614.60885717
GACGTTA400.005293682714.2436357
TGGTATC2250.013.5161952
CGCCACC500.001504195513.2940614
CCACCTT5650.013.10918613
TCCACCT5500.012.7761112
GGTATCA7400.012.6339931
CACCTTT6000.012.34448414
TTCCACC5650.012.26885411
ACCTTTT6050.012.24246315
GTGGTAT2750.011.7948521
TAGGACC6300.011.464634
GGCGAGG9350.011.27450219
TGTCGAG855.3441418E-511.17147818
GGACGTG19500.011.1075416
ATTAGGC600.00585767911.0875033
Produced by FastQC (version 0.11.5)