FastQCFastQC Report
Thu 26 May 2016
SRR1512047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2079282
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34530.16606694041500863No Hit
GTCCTACAGTGGACATTTCTAAATT32930.15837197648034274No Hit
CTGTAGGACGTGGAATATGGCAAGA31460.1513022283653684No Hit
GTCCTAAAGTGTGTATTTCTCATTT22860.10994179721653917No Hit
GTCCTACAGTGTGCATTTCTCATTT21540.10359345197043979No Hit
GGTATCAACGCAGAGTACTTTTTTT21150.10171780451136499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACA551.4964602E-414.2649391
CACGACT400.005410947414.1974274
CGGTAGG1253.6379788E-1214.1222921
CCTACCG552.0230879E-413.7672013
TAGGACC7600.013.5747334
CTACCGT604.2226652E-412.6199354
GCCGGTT802.9779661E-511.83118911
ATCGTGC658.278114E-411.6491718
GTCAACG600.00463615911.441672
GTACTAG1353.5648554E-810.896831
GTAGGAC19300.010.8871473
GTCCTAC18850.010.8737061
ATAGGAC3600.010.7795283
TGTAGGA19250.010.6987052
GGTATCA9850.010.6534481
GTTAGGC803.90285E-410.648073
AGTCGTC909.881035E-510.5166128
GGCGAGG6250.010.44980919
AGGACCT13400.010.3831935
GGACCTG12700.010.1356956
Produced by FastQC (version 0.11.5)