##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512047_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2079282 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.506220416470686 31.0 30.0 34.0 25.0 34.0 2 26.535352107121593 31.0 25.0 34.0 10.0 34.0 3 28.521023603339998 31.0 28.0 34.0 16.0 34.0 4 33.01881947710796 35.0 33.0 37.0 27.0 37.0 5 33.48268729301749 35.0 35.0 37.0 28.0 37.0 6 33.773878194492134 37.0 35.0 37.0 29.0 37.0 7 33.841897828192614 37.0 35.0 37.0 29.0 37.0 8 33.87289602853293 37.0 35.0 37.0 30.0 37.0 9 35.502781729462384 39.0 37.0 39.0 30.0 39.0 10 35.41527027118015 39.0 37.0 39.0 30.0 39.0 11 35.427526424987086 39.0 37.0 39.0 30.0 39.0 12 35.323396249282204 39.0 37.0 39.0 30.0 39.0 13 35.33123164630868 39.0 37.0 39.0 30.0 39.0 14 36.545663358794044 40.0 37.0 41.0 30.0 41.0 15 36.50182418738776 40.0 37.0 41.0 29.0 41.0 16 36.44560766649257 40.0 37.0 41.0 29.0 41.0 17 36.41511348628998 40.0 37.0 41.0 29.0 41.0 18 36.35359898272577 40.0 37.0 41.0 29.0 41.0 19 36.38858605999571 40.0 37.0 41.0 29.0 41.0 20 36.28943164034508 40.0 37.0 41.0 28.0 41.0 21 36.2334426018212 40.0 37.0 41.0 28.0 41.0 22 36.13421219440172 40.0 37.0 41.0 28.0 41.0 23 36.04513433002354 40.0 37.0 41.0 27.0 41.0 24 35.95420149840185 40.0 37.0 41.0 27.0 41.0 25 35.8658801451655 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1918888264522494 1101 2 4.142741394856827 1101 3 2.31822027180597 1101 4 1.403232144234586 1101 5 0.9166992341408005 1101 6 0.7469719011685854 1101 7 0.34530000122349236 1101 8 0.35906850300024473 1101 9 0.3634697844257815 1101 10 0.4152750390660671 1101 11 0.483603952627476 1101 12 0.3005304442395982 1101 13 0.4444989432218378 1101 14 0.241602990361514 1101 15 0.30952626036689423 1101 16 0.4731964307890948 1101 17 0.4793198006584731 1101 18 0.6565151718054594 1101 19 0.5654865422838213 1101 20 0.5024840737639593 1101 21 0.4875758446882301 1101 22 0.34183145723498143 1101 23 0.477367028745725 1101 24 0.4008500505660635 1101 25 0.20942470048293416 1102 1 1.8838731944234617 1102 2 4.631368812760563 1102 3 2.867042246614787 1102 4 2.0025630034338633 1102 5 1.5277400573526734 1102 6 1.3828524679702028 1102 7 1.113757603562675 1102 8 1.1647505767744235 1102 9 1.2390180300398157 1102 10 1.0878458892685003 1102 11 1.0844670880300882 1102 12 1.11989054320496 1102 13 1.1429216827630029 1102 14 1.2498407321518883 1102 15 1.186452148805941 1102 16 1.2803686080270609 1102 17 1.376755698094371 1102 18 1.3924294767987178 1102 19 1.3646880717485104 1102 20 1.3764156976955846 1102 21 1.2543746526054562 1102 22 1.3471733375768622 1102 23 1.4781120804776222 1102 24 1.4475071580784373 1102 25 1.3928480023272911 1103 1 1.2701822842278112 1103 2 4.121209113323612 1103 3 2.4432817653898518 1103 4 1.6198160110897177 1103 5 1.1620161945908976 1103 6 1.0009282771005132 1103 7 0.7189445440576705 1103 8 0.7484813865123456 1103 9 0.8456860131888391 1103 10 0.7664571016474824 1103 11 0.8097096245877893 1103 12 0.7097936298497487 1103 13 0.766055822188612 1103 14 0.8361437458838097 1103 15 1.0350440190210293 1103 16 0.8486788522851754 1103 17 0.8861457616905994 1103 18 0.9615042935038645 1103 19 0.8772503514908792 1103 20 0.8523392607044542 1103 21 1.0114102381363068 1103 22 1.0858085504419321 1103 23 1.2139345781484607 1103 24 1.2719187917317711 1103 25 1.2544553260199223 1104 1 1.334340060130053 1104 2 3.8216739097437404 1104 3 2.2294754121301423 1104 4 1.548444633219603 1104 5 0.9852733282421084 1104 6 0.9247069541740061 1104 7 0.712565315384083 1104 8 0.7050098165199685 1104 9 0.7060715561686877 1104 10 0.6686863451089522 1104 11 0.6041003276975729 1104 12 0.8182645626406213 1104 13 0.7244621932418696 1104 14 0.7665469140977947 1104 15 0.701797248911312 1104 16 0.8669460970018079 1104 17 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-1.094109362751034 2315 14 -1.1267044748989932 2315 15 -1.4946804106111173 2315 16 -1.4960095769050028 2315 17 -1.3690340115658444 2315 18 -1.239408533961793 2315 19 -1.275499429261302 2315 20 -1.1558807655555086 2315 21 -1.2055850762814941 2315 22 -1.4380598471128465 2315 23 -1.3874244099170951 2315 24 -1.3089572240003164 2315 25 -1.2649178193326733 2316 1 -0.5172355356469183 2316 2 -0.16047333970026045 2316 3 -0.018044707755812794 2316 4 -0.4242632857296158 2316 5 -0.6082622061821468 2316 6 -0.6100113674225796 2316 7 -0.713224226313514 2316 8 -0.9338144596524032 2316 9 -0.8810072955752375 2316 10 -0.8557280837917247 2316 11 -1.1332643986051636 2316 12 -0.9810694491262382 2316 13 -1.1633721500322807 2316 14 -1.1930042404506835 2316 15 -1.126519420949279 2316 16 -1.252376886549115 2316 17 -1.4083128573251216 2316 18 -1.1725543816004844 2316 19 -1.1519900362827116 2316 20 -1.0982756946875583 2316 21 -1.1947646166314954 2316 22 -1.3176294689589412 2316 23 -1.2041705026732714 2316 24 -1.2775630362392292 2316 25 -1.0883448681564616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69661.0 3 3.0 4 640.0 5 2831.0 6 3291.0 7 3062.0 8 2819.0 9 2714.0 10 2728.0 11 2600.0 12 2507.0 13 2572.0 14 2558.0 15 2687.0 16 2728.0 17 3062.0 18 3602.0 19 4234.0 20 5704.0 21 7322.0 22 9014.0 23 10620.0 24 12264.0 25 14135.0 26 15873.0 27 18052.0 28 20856.0 29 25467.0 30 31605.0 31 39892.0 32 51928.0 33 69481.0 34 95816.0 35 140866.0 36 233167.0 37 323857.0 38 839064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.073618681833445 18.853767790997082 12.759789196463009 25.312824330706462 2 17.002712385030105 21.175190771412666 39.34433336771778 22.477763475839442 3 19.02118135010066 23.79436747877392 29.570159314609562 27.614291856515855 4 12.051179205129463 16.477178179775517 38.774682799158555 32.69695981593646 5 13.068934372538212 37.64092605043472 36.188405420717345 13.101734156309726 6 31.88254407050126 37.54305572789068 17.967788880969486 12.606611320638567 7 28.412596271212852 31.43431242130697 21.875916782812528 18.27717452466765 8 24.811930272084307 37.91073072339394 20.22967543603994 17.047663568481813 9 25.96607867523501 15.835754842296524 20.462255720965217 37.73591076150325 10 14.252625908968488 29.616694240313958 34.93877688430611 21.19190296641145 11 34.5032564125501 22.71399454234683 24.337487651987562 18.44526139311551 12 22.862940188007205 24.793558545690292 32.03187446435837 20.311626801944133 13 28.85462385573482 22.04501361527681 25.81362220227944 23.286740326708934 14 20.278153708828338 22.279758108808714 27.93824983816529 29.503838344197657 15 22.580150263408232 31.265840804662375 23.401154821712495 22.75285411021689 16 21.830468402073407 28.708948569746674 27.977205593084538 21.48337743509538 17 21.417200745257254 29.010975904182306 28.008129729396973 21.56369362116346 18 21.150459246833268 28.07994521164599 29.991573982072588 20.778021559448153 19 23.078621164624757 27.445170449900015 27.751721524271595 21.724486861203637 20 22.957646633334424 27.69320741722568 28.592081444046656 20.757064505393245 21 22.488251598316484 27.232773459649994 27.50359630226542 22.775378639768103 22 22.520589214156683 28.372235293212338 27.889271292835282 21.217904199795694 23 21.962477035099027 28.042572838413665 28.123082250416015 21.871867876071292 24 22.48609014366024 27.192188068856492 28.94192752206171 21.379794265421555 25 22.27821935840075 27.769225108968378 28.40839081736742 21.54416471526346 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 139.0 2 300.0 3 461.0 4 461.0 5 461.0 6 1136.5 7 1812.0 8 1812.0 9 1812.0 10 3072.0 11 4332.0 12 4332.0 13 4332.0 14 7419.0 15 10506.0 16 10506.0 17 10506.0 18 18333.5 19 26161.0 20 26161.0 21 26161.0 22 40476.0 23 54791.0 24 54791.0 25 54791.0 26 77126.0 27 99461.0 28 99461.0 29 99461.0 30 127855.5 31 156250.0 32 156250.0 33 156250.0 34 180191.0 35 204132.0 36 204132.0 37 204132.0 38 221506.0 39 238880.0 40 238880.0 41 238880.0 42 251302.0 43 263724.0 44 263724.0 45 263724.0 46 267882.5 47 272041.0 48 272041.0 49 272041.0 50 262128.0 51 252215.0 52 252215.0 53 252215.0 54 223669.0 55 195123.0 56 195123.0 57 195123.0 58 166859.5 59 138596.0 60 138596.0 61 138596.0 62 111687.5 63 84779.0 64 84779.0 65 84779.0 66 65039.5 67 45300.0 68 45300.0 69 45300.0 70 32523.0 71 19746.0 72 19746.0 73 19746.0 74 13552.5 75 7359.0 76 7359.0 77 7359.0 78 4873.0 79 2387.0 80 2387.0 81 2387.0 82 1549.5 83 712.0 84 712.0 85 712.0 86 477.5 87 243.0 88 243.0 89 243.0 90 163.0 91 83.0 92 83.0 93 83.0 94 60.0 95 37.0 96 37.0 97 37.0 98 24.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.489714237895581 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 9.618704918332387E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.847481967332955E-4 19 9.618704918332385E-4 20 6.73309344283267E-4 21 0.0030298920492747012 22 0.0018275539344831532 23 0.0010580575410165625 24 4.8093524591661935E-5 25 4.3284172132495734E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2079282.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.8357911880898 #Duplication Level Percentage of deduplicated Percentage of total 1 84.58584933388768 64.14634807551931 2 10.871236137992458 16.488575874344235 3 2.596282918198692 5.9067350774916125 4 0.8947813940318846 2.714258198271596 5 0.3726558076634601 1.413032400749756 6 0.201087228222388 0.9149765430038866 7 0.11370448254957022 0.6036008577045304 8 0.0687346736452825 0.41700386863561145 9 0.048816121853489707 0.33318083011442334 >10 0.22122319133277749 2.916066122349723 >50 0.013385781357443203 0.6898295727014944 >100 0.010193401354477927 1.52394216477696 >500 0.0011528594496199105 0.5822705365473284 >1k 8.966684608153945E-4 1.3501798777895662 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3453 0.16606694041500863 No Hit GTCCTACAGTGGACATTTCTAAATT 3293 0.15837197648034274 No Hit CTGTAGGACGTGGAATATGGCAAGA 3146 0.1513022283653684 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2286 0.10994179721653917 No Hit GTCCTACAGTGTGCATTTCTCATTT 2154 0.10359345197043979 No Hit GGTATCAACGCAGAGTACTTTTTTT 2115 0.10171780451136499 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 4.809352459166193E-5 4.809352459166193E-5 11 0.0 0.0 0.0 4.809352459166193E-5 9.618704918332386E-5 12 0.0 0.0 0.0 4.809352459166193E-5 9.618704918332386E-5 13 0.0 0.0 0.0 4.809352459166193E-5 9.618704918332386E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTACA 55 1.4964602E-4 14.264939 1 CACGACT 40 0.0054109474 14.197427 4 CGGTAGG 125 3.6379788E-12 14.122292 1 CCTACCG 55 2.0230879E-4 13.767201 3 TAGGACC 760 0.0 13.574733 4 CTACCGT 60 4.2226652E-4 12.619935 4 GCCGGTT 80 2.9779661E-5 11.831189 11 ATCGTGC 65 8.278114E-4 11.649171 8 GTCAACG 60 0.004636159 11.44167 2 GTACTAG 135 3.5648554E-8 10.89683 1 GTAGGAC 1930 0.0 10.887147 3 GTCCTAC 1885 0.0 10.873706 1 ATAGGAC 360 0.0 10.779528 3 TGTAGGA 1925 0.0 10.698705 2 GGTATCA 985 0.0 10.653448 1 GTTAGGC 80 3.90285E-4 10.64807 3 AGTCGTC 90 9.881035E-5 10.516612 8 GGCGAGG 625 0.0 10.449809 19 AGGACCT 1340 0.0 10.383193 5 GGACCTG 1270 0.0 10.135695 6 >>END_MODULE