FastQCFastQC Report
Thu 26 May 2016
SRR1512047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2079282
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45880.22065309082654494No Hit
GTCCTACAGTGGACATTTCTAAATT38570.18549672435004005No Hit
CTGTAGGACGTGGAATATGGCAAGA36310.17462758779232446No Hit
GGTATCAACGCAGAGTACTTTTTTT28100.13514280410257No Hit
GTCCTAAAGTGTGTATTTCTCATTT26520.12754402721708744No Hit
TATCAACGCAGAGTACTTTTTTTTT26490.12739974664331247No Hit
GTCCTACAGTGTGCATTTCTCATTT23880.11484733672488869No Hit
CTTTAGGACGTGAAATATGGCGAGG23400.11253884754448891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7750.012.3853984
TAGGACA5950.012.1391114
GGACCGG550.003068521212.0911856
GGTATCA10700.011.8660641
AGGACCG907.432018E-611.6155685
ACTGTTC5450.011.4987638
GTAGGAC19450.011.1893833
GGCGAGG7600.011.12070519
GCGCCAC1303.292007E-810.9559913
GTCCTAA9200.010.89532851
GTCCTAC19950.010.86231
TGTAGGA21750.010.7926472
CTGTAGG20750.010.7655591
GGACGTG18150.010.4685586
CTAGGAC3450.010.4677843
AGGACGT18350.010.4099885
TGTCGAG1106.0803904E-610.35888818
TAGAACA7650.010.1869154
GTCTTAG2350.010.1557261
TGGCGAG18350.010.09074218
Produced by FastQC (version 0.11.5)