FastQCFastQC Report
Thu 26 May 2016
SRR1512046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214033
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34900.1576308934871341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.012.29303551
GGCGAGG5700.011.78992719
ATAGGAC2150.011.4459723
GGCGTGC1701.4915713E-1010.5784618
TGGCGAG12050.010.44701618
CCTATAC2151.8189894E-1210.1252833
CTCAACG800.00346939579.8082722
CGGTAGG908.314032E-49.8082721
GTATCAA21100.09.6688181
GGACGTG11850.09.6646216
TAGGACG12450.09.5789744
ATGGCGA13650.09.56916517
GTATTAG2052.3101165E-109.5690471
TATGGCG13550.09.5000816
GTGGTAT4450.09.4776571
GTACTAG1451.1053344E-69.4700571
CGTGCGC1002.860262E-49.46493910
TGGACCG1002.860262E-49.4649395
CACAGCG1002.860262E-49.4649397
ACGACAG800.0046565799.4649395
Produced by FastQC (version 0.11.5)