FastQCFastQC Report
Thu 26 May 2016
SRR1512046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214033
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46760.21119829740568458No Hit
TATCAACGCAGAGTACTTTTTTTTT30040.135680001156261No Hit
GGTATCAACGCAGAGTACTTTTTTT28290.12777587325934167No Hit
GTCCTACAGTGGACATTTCTAAATT24500.11065779055687065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5450.013.6028984
GGTATCA10500.012.5499071
AGGTCGA658.0633326E-411.6858037
GGCGAGG6500.011.54129519
CCTTGCG803.7914803E-410.6815559
GTAGGAC12800.010.6184083
CGTGCGC909.572184E-510.54968410
GTATCAA23250.010.5139951
ACCGTGC1106.0880866E-610.3578728
TCGAACT1303.8653343E-710.22646319
AGTGCGG1303.8725375E-710.22507810
TGACCCG856.566078E-410.0636835
GGACGTG12400.010.0368276
AGGACGT12700.010.0284645
TGTAGGA13800.09.9178322
GTCCTAA7250.09.8781131
CAGTGCG1451.6308877E-79.822129
TTAGGAC9050.09.7671473
TGGCGAG13850.09.73465418
TAGGACA5500.09.67744
Produced by FastQC (version 0.11.5)