FastQCFastQC Report
Thu 26 May 2016
SRR1512045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249198
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37420.16637041292051657No Hit
GTCCTACAGTGGACATTTCTAAATT23880.10617117745969898No Hit
TATCAACGCAGAGTACTTTTTTTTT23080.10261435409421492No Hit
GGTATCAACGCAGAGTACTTTTTTT22740.1011027041638842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG500.001186461713.7198541
AATCCCG1351.8189894E-1213.32240619
GGTATCA10100.012.6137281
TAGGACC5350.012.031454
GTAGGAC14150.010.77041153
GGCGAGG7200.010.25474619
TGTAGGA14850.010.22884752
GGCCTAA1157.0930546E-610.2259791
TTATGCT1951.6370905E-1110.1940684
TAGGACG15500.010.1376964
AGGACGT15400.010.080595
GGACGTG15650.09.9195596
TTAGGAC10050.09.8897683
GTCCTAC14300.09.8684271
GTATTAT3300.09.7998961
GTATCAA20150.09.7269451
GACGTGG8350.09.5226037
TCAAGAC2305.456968E-129.465923
AAAGGCG1502.791021E-79.46591955
GTCTTAT2405.456968E-129.3915681
Produced by FastQC (version 0.11.5)