##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512045_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2249198 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.854080876828096 31.0 30.0 34.0 25.0 34.0 2 26.884245851187845 31.0 25.0 34.0 10.0 34.0 3 28.871333248562376 31.0 28.0 34.0 16.0 34.0 4 33.419119615080575 35.0 33.0 37.0 28.0 37.0 5 33.87555875472057 37.0 35.0 37.0 30.0 37.0 6 34.16289628569828 37.0 35.0 37.0 30.0 37.0 7 34.241077041683305 37.0 35.0 37.0 30.0 37.0 8 34.28541062191946 37.0 35.0 37.0 30.0 37.0 9 35.949167658872184 39.0 37.0 39.0 31.0 39.0 10 35.85835884613093 39.0 37.0 39.0 31.0 39.0 11 35.87539425163992 39.0 37.0 39.0 31.0 39.0 12 35.777873713207995 39.0 37.0 39.0 30.0 39.0 13 35.781156661174336 39.0 37.0 39.0 30.0 39.0 14 37.0239071882511 40.0 38.0 41.0 31.0 41.0 15 36.968542564949814 40.0 38.0 41.0 31.0 41.0 16 36.918923545192555 40.0 37.0 41.0 31.0 41.0 17 36.89071793590426 40.0 37.0 41.0 31.0 41.0 18 36.8370130153059 40.0 37.0 41.0 31.0 41.0 19 36.86982026482328 40.0 37.0 41.0 31.0 41.0 20 36.77863042737901 40.0 37.0 41.0 30.0 41.0 21 36.72002331497716 40.0 37.0 41.0 30.0 41.0 22 36.626373934175646 40.0 37.0 41.0 30.0 41.0 23 36.54355552512496 40.0 37.0 41.0 30.0 41.0 24 36.45196332203746 40.0 37.0 41.0 30.0 41.0 25 36.357743071085785 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.175570800380406 1101 2 4.244666893277401 1101 3 2.3247159538506423 1101 4 1.3568448592182136 1101 5 0.9832294135291306 1101 6 0.555125681321428 1101 7 0.3690271292685665 1101 8 0.1750881323716058 1101 9 0.22160531878856204 1101 10 0.5234192711169499 1101 11 0.42794349505894047 1101 12 0.3059273528525708 1101 13 0.4193572757072346 1101 14 0.4051522658611404 1101 15 0.42593014332188517 1101 16 0.4440413521592603 1101 17 0.5120343431122905 1101 18 0.5518440423542756 1101 19 0.39942168417685053 1101 20 0.37239378987891314 1101 21 0.44028938924524397 1101 22 0.5766051961605001 1101 23 0.5493864517029579 1101 24 0.413669189540677 1101 25 0.35872027319725674 1102 1 1.3891743149435456 1102 2 4.19861951326051 1102 3 2.399906535066261 1102 4 1.577391161510242 1102 5 1.010126050475769 1102 6 0.8592991207185605 1102 7 0.7451877671908065 1102 8 0.6651358991035821 1102 9 0.6044866382938139 1102 10 0.5030052124007085 1102 11 0.6938743365838889 1102 12 0.6255323006726954 1102 13 0.7033549915843196 1102 14 0.8318070435892295 1102 15 0.730909007302273 1102 16 0.669780023180877 1102 17 0.7338831426146655 1102 18 0.8438582135610204 1102 19 0.7662520092238623 1102 20 0.698015542185118 1102 21 0.7544800940334895 1102 22 0.6355294002357041 1102 23 0.7101103879060986 1102 24 0.6721518243409079 1102 25 0.5781201090056314 1103 1 1.1275382767907658 1103 2 3.9412281116213954 1103 3 2.168836977796939 1103 4 1.272670156638732 1103 5 0.8375948963731545 1103 6 0.7443551218590656 1103 7 0.4259052476853711 1103 8 0.345925563457655 1103 9 0.46646342937838625 1103 10 0.3113512004669161 1103 11 0.516437838078545 1103 12 0.3251249581305018 1103 13 0.47866513404819244 1103 14 0.46913503170776494 1103 15 0.6342311340914222 1103 16 0.4884830416391921 1103 17 0.5396875821024807 1103 18 0.6656507012063031 1103 19 0.535973993088966 1103 20 0.6668909898378388 1103 21 0.701987991398866 1103 22 0.6798593129563955 1103 23 0.7231670946438271 1103 24 0.8434748010949988 1103 25 0.7427762296104277 1104 1 1.3577037009622863 1104 2 4.051229744096773 1104 3 2.295504779589855 1104 4 1.6013725844631352 1104 5 1.1505474475108883 1104 6 0.8026094790762741 1104 7 0.7564916047551336 1104 8 0.7512757820864877 1104 9 0.6697759501134541 1104 10 0.742284326903011 1104 11 0.6962672882854122 1104 12 0.7723165738773972 1104 13 0.7680055964424568 1104 14 0.7044949749748568 1104 15 0.727299345231927 1104 16 0.8605970270381889 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-0.6517241484476131 2315 13 -0.6625704932672534 2315 14 -0.8009959037309287 2315 15 -1.099910909822917 2315 16 -0.9114559662220145 2315 17 -0.7328639788444775 2315 18 -0.7660331618550487 2315 19 -0.7809387599154576 2315 20 -0.7180689417154298 2315 21 -0.7709820433892389 2315 22 -0.9577174919591158 2315 23 -0.959810027656701 2315 24 -0.8849859380393639 2315 25 -0.7626064678233888 2316 1 -0.28068006344572893 2316 2 0.13815066028332623 2316 3 0.21994507211562464 2316 4 -0.021623675097629302 2316 5 -0.3985451846371788 2316 6 -0.5104669385393947 2316 7 -0.5445121680834646 2316 8 -0.7842079353762017 2316 9 -0.6880318413264277 2316 10 -0.8293732820035729 2316 11 -0.79368907719428 2316 12 -0.7612635121226674 2316 13 -0.8390228383880753 2316 14 -1.0345949606010265 2316 15 -0.8376760633122586 2316 16 -1.029967197785055 2316 17 -1.0017159901889627 2316 18 -1.0810594220398997 2316 19 -0.9040015487394868 2316 20 -0.7970982812421923 2316 21 -0.9553220804803289 2316 22 -1.0828597323649447 2316 23 -1.0032776370189467 2316 24 -1.0445970462549994 2316 25 -1.02817867057351 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58751.0 3 5.0 4 513.0 5 2355.0 6 2934.0 7 2654.0 8 2379.0 9 2419.0 10 2378.0 11 2344.0 12 2272.0 13 2413.0 14 2305.0 15 2513.0 16 2608.0 17 2815.0 18 3246.0 19 4075.0 20 5182.0 21 6728.0 22 8057.0 23 9932.0 24 11732.0 25 13278.0 26 15272.0 27 17587.0 28 20734.0 29 25522.0 30 32516.0 31 41852.0 32 55788.0 33 75450.0 34 106447.0 35 158026.0 36 260852.0 37 365727.0 38 919537.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.631773636647374 18.87864029756384 13.037536046181794 25.452050019606986 2 17.160984573009856 21.61845310316512 38.630626782462684 22.58993554136234 3 18.35107447187842 24.693601897209582 29.819162208040378 27.13616142287162 4 12.387170893803036 17.154470171145448 38.34593486211529 32.11242407293622 5 13.460575725214055 37.38621499752356 35.71326312756814 13.439946149694245 6 31.417640533613138 37.2365668605879 18.405607339514805 12.94018526628416 7 27.77443337580773 31.621760289667694 22.64789493855143 17.955911395973143 8 25.040458901758672 36.53394368476558 20.898756711287056 17.526840702188693 9 26.297373552706343 15.697195178014564 20.441552944649604 37.56387832462949 10 14.93850021896723 29.013447033271905 34.41404591420486 21.634006833556004 11 33.91893466026557 22.816266064615032 24.600857727954587 18.66394154716481 12 22.9665863120988 24.810221243305392 31.753095992438197 20.470096452157613 13 28.57360712573993 22.00988974736773 26.023320312395796 23.393182814496544 14 21.06164063812968 21.904696696333538 27.830764565858583 29.202898099678197 15 22.957071809596133 30.36117762864808 23.61139392796899 23.070356633786798 16 22.348632712638018 28.163683232867893 27.28399189399955 22.20369216049454 17 21.94911252811002 28.407636855448033 27.602994489591403 22.04025612685055 18 21.736139979592608 27.664909733970305 29.23205516664925 21.366895119787834 19 23.338185497926347 27.121406671266886 27.589401947742452 21.951005883064315 20 23.47300948479423 27.239539405267433 27.96202525454844 21.325425855389902 21 23.126039570019834 26.796619310514703 27.199086578925957 22.87825454053951 22 23.1819782424278 27.614945748345598 27.428252831738952 21.774823177487654 23 22.604497762056376 27.50583216956708 27.590041273131384 22.299628795245162 24 22.96530717407146 26.91347178570841 28.109676475648865 22.011544564571267 25 22.860208102600993 27.20028063573419 27.747146847805354 22.19236441385946 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 188.0 1 188.0 2 386.5 3 585.0 4 585.0 5 585.0 6 1384.5 7 2184.0 8 2184.0 9 2184.0 10 3299.0 11 4414.0 12 4414.0 13 4414.0 14 7582.5 15 10751.0 16 10751.0 17 10751.0 18 19396.5 19 28042.0 20 28042.0 21 28042.0 22 42712.5 23 57383.0 24 57383.0 25 57383.0 26 80901.5 27 104420.0 28 104420.0 29 104420.0 30 133222.0 31 162024.0 32 162024.0 33 162024.0 34 186542.5 35 211061.0 36 211061.0 37 211061.0 38 230183.5 39 249306.0 40 249306.0 41 249306.0 42 263742.5 43 278179.0 44 278179.0 45 278179.0 46 284360.5 47 290542.0 48 290542.0 49 290542.0 50 284399.0 51 278256.0 52 278256.0 53 278256.0 54 250197.0 55 222138.0 56 222138.0 57 222138.0 58 190194.5 59 158251.0 60 158251.0 61 158251.0 62 128835.5 63 99420.0 64 99420.0 65 99420.0 66 76452.0 67 53484.0 68 53484.0 69 53484.0 70 38832.0 71 24180.0 72 24180.0 73 24180.0 74 16760.0 75 9340.0 76 9340.0 77 9340.0 78 6425.5 79 3511.0 80 3511.0 81 3511.0 82 2287.5 83 1064.0 84 1064.0 85 1064.0 86 694.0 87 324.0 88 324.0 89 324.0 90 209.5 91 95.0 92 95.0 93 95.0 94 69.5 95 44.0 96 44.0 97 44.0 98 28.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4075257047178593 3 0.0 4 0.0 5 0.0 6 1.3338087620565195E-4 7 0.0 8 8.892058413710132E-5 9 0.0 10 1.3338087620565195E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 5.779837968911585E-4 19 0.0013338087620565198 20 8.447455493024624E-4 21 0.0031566807368670967 22 0.002223014603427533 23 0.0015561102223992731 24 4.446029206855066E-5 25 8.447455493024624E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2249198.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.98462232353879 #Duplication Level Percentage of deduplicated Percentage of total 1 81.66793673939718 59.6050351886756 2 12.454337052563236 18.17950172142766 3 3.2899227092251326 7.203412992192895 4 1.1867032936697135 3.4644436679433426 5 0.5312630789955453 1.9387017587465114 6 0.26012586915701985 1.1391112990204415 7 0.16592901330441273 0.8477186457978004 8 0.0956754365506179 0.5586268481829237 9 0.06814652754185636 0.4476283717772718 >10 0.25741093995448594 3.1079169167889686 >50 0.011588929083977371 0.5730031620430769 >100 0.009238807903303082 1.3798396235252421 >500 9.222871358298092E-4 0.4898126499680392 >1k 7.993155177179612E-4 1.0652471539100838 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3742 0.16637041292051657 No Hit GTCCTACAGTGGACATTTCTAAATT 2388 0.10617117745969898 No Hit TATCAACGCAGAGTACTTTTTTTTT 2308 0.10261435409421492 No Hit GGTATCAACGCAGAGTACTTTTTTT 2274 0.1011027041638842 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3338087620565198E-4 0.0 10 0.0 0.0 0.0 1.7784116827420263E-4 0.0 11 0.0 0.0 0.0 1.7784116827420263E-4 0.0 12 0.0 0.0 0.0 1.7784116827420263E-4 0.0 13 0.0 0.0 0.0 1.7784116827420263E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 50 0.0011864617 13.719854 1 AATCCCG 135 1.8189894E-12 13.322406 19 GGTATCA 1010 0.0 12.613728 1 TAGGACC 535 0.0 12.03145 4 GTAGGAC 1415 0.0 10.7704115 3 GGCGAGG 720 0.0 10.254746 19 TGTAGGA 1485 0.0 10.2288475 2 GGCCTAA 115 7.0930546E-6 10.225979 1 TTATGCT 195 1.6370905E-11 10.194068 4 TAGGACG 1550 0.0 10.137696 4 AGGACGT 1540 0.0 10.08059 5 GGACGTG 1565 0.0 9.919559 6 TTAGGAC 1005 0.0 9.889768 3 GTCCTAC 1430 0.0 9.868427 1 GTATTAT 330 0.0 9.799896 1 GTATCAA 2015 0.0 9.726945 1 GACGTGG 835 0.0 9.522603 7 TCAAGAC 230 5.456968E-12 9.46592 3 AAAGGCG 150 2.791021E-7 9.4659195 5 GTCTTAT 240 5.456968E-12 9.391568 1 >>END_MODULE