FastQCFastQC Report
Thu 26 May 2016
SRR1512045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249198
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46890.20847430950943405No Hit
TATCAACGCAGAGTACTTTTTTTTT29790.1324472100722124No Hit
GGTATCAACGCAGAGTACTTTTTTT29350.1304909572211962No Hit
GTCCTACAGTGGACATTTCTAAATT26710.1187534401150988No Hit
CTGTAGGACGTGGAATATGGCAAGA24650.10959461994897736No Hit
GTCCTAAAGTGTGTATTTCTCATTT23280.10350355993558594No Hit
CTTTAGGACGTGAAATATGGCGAGG23140.10288111584662622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG751.47913615E-512.6667856
GGTATCA10500.011.9992831
TAGGACC6150.011.59114554
GTATTAG2050.011.17449951
CCGGTTT700.001498390110.85145212
ATAGGAC2750.010.7144353
GTTACCC909.470479E-510.5608223
GGCGAGG6750.010.55096119
CTAGTAC1106.0120437E-610.3688083
GTATCAA23500.010.2353671
GTGCTAG2550.010.1063481
GTCCTAA8200.09.7776871
TGGCGAG15100.09.7467618
ATAGACC2054.0017767E-119.7365633
GTCTTAG2550.09.73203851
AGGACGT15300.09.5668625
TTAGGAC11050.09.5477483
GTGTACG1201.6085913E-59.5448851
TAGGACA6600.09.5047414
AAGTCGT1106.8776695E-59.4950217
Produced by FastQC (version 0.11.5)