FastQCFastQC Report
Thu 26 May 2016
SRR1512044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219315
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38630.17406271755023509No Hit
GTCCTACAGTGGACATTTCTAAATT26270.11836985736589895No Hit
GGTATCAACGCAGAGTACTTTTTTT23440.10561817497741421No Hit
CTGTAGGACGTGGAATATGGCAAGA22740.10246404859156992No Hit
TATCAACGCAGAGTACTTTTTTTTT22480.10129251593397062No Hit
GTCCTAAAGTGTGTATTTCTCATTT22310.10052651381169414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAC450.00934962613.0711841
TAGGACC5800.012.5662164
CGGTAGG1208.258212E-811.4372861
GGTATCA9700.011.1172441
TCGAACT1303.446985E-810.92193319
GTATCAA19150.010.8528371
GTAGGAC15300.010.7646443
GTCAACG750.002039698210.4569472
TAGGACA6450.010.4193464
GGCGAGG6350.010.13647119
GTGGTAT3400.010.09172251
CTACACT3300.010.0391264
CGTGCGC951.7060922E-49.96364410
AGGACCT9700.09.9533735
TGGCGAG12850.09.94448418
TCCAACG2002.7284841E-119.9389618
GTCCTAA7600.09.932381
GCGTGCG1054.2739826E-59.9161999
GTGCGCC1054.2739826E-59.91619911
TCTATAC2300.09.8770043
Produced by FastQC (version 0.11.5)