##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512044_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2219315 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.590550237348012 31.0 30.0 34.0 25.0 34.0 2 26.627841023018362 31.0 25.0 34.0 10.0 34.0 3 28.605249367485012 31.0 28.0 34.0 16.0 34.0 4 33.12547430175527 35.0 33.0 37.0 28.0 37.0 5 33.589742780993234 35.0 35.0 37.0 28.0 37.0 6 33.87539308300084 37.0 35.0 37.0 30.0 37.0 7 33.948474641950334 37.0 35.0 37.0 30.0 37.0 8 33.99558061834395 37.0 35.0 37.0 30.0 37.0 9 35.62819338399461 39.0 37.0 39.0 30.0 39.0 10 35.53902623106679 39.0 37.0 39.0 30.0 39.0 11 35.55356900665296 39.0 37.0 39.0 30.0 39.0 12 35.448198655891574 39.0 37.0 39.0 30.0 39.0 13 35.451575373482356 39.0 37.0 39.0 30.0 39.0 14 36.66371921065734 40.0 37.0 41.0 30.0 41.0 15 36.598538738304384 40.0 37.0 41.0 30.0 41.0 16 36.550005294426434 40.0 37.0 41.0 30.0 41.0 17 36.514742612022175 40.0 37.0 41.0 30.0 41.0 18 36.46224353009825 40.0 37.0 41.0 30.0 41.0 19 36.491496249969025 40.0 37.0 41.0 30.0 41.0 20 36.39972288746753 40.0 37.0 41.0 29.0 41.0 21 36.34635597019801 40.0 37.0 41.0 29.0 41.0 22 36.241530832711895 40.0 37.0 41.0 29.0 41.0 23 36.147575715930365 40.0 37.0 41.0 29.0 41.0 24 36.0563349501986 40.0 37.0 41.0 28.0 41.0 25 35.956070228876925 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2445789133456877 1101 2 4.205828928721061 1101 3 2.331921549652943 1101 4 1.4893516455506344 1101 5 1.0219710036942118 1101 6 0.6755921054888674 1101 7 0.27769874867701816 1101 8 0.14336053050993058 1101 9 0.3330008075663926 1101 10 0.4032469421983862 1101 11 0.509185014617735 1101 12 0.306876085715281 1101 13 0.490270766582384 1101 14 0.2687041502031704 1101 15 0.42164779488815896 1101 16 0.645455897627933 1101 17 0.56248206759998 1101 18 0.6615692145704841 1101 19 0.5574304240308479 1101 20 0.5167521452262065 1101 21 0.593976695547326 1101 22 0.6812004777758816 1101 23 0.7580470574149629 1101 24 0.7123460333289842 1101 25 0.5330915705339976 1102 1 1.7218334185688278 1102 2 4.358956636923253 1102 3 2.6404429136474477 1102 4 1.8739986005690739 1102 5 1.3596865552385822 1102 6 1.2646765948374963 1102 7 0.9211701883062489 1102 8 0.9362057898549878 1102 9 0.938303629901398 1102 10 0.7527588376806307 1102 11 0.9316478476562082 1102 12 0.898244840342663 1102 13 0.925707968966357 1102 14 1.0602317736489155 1102 15 1.0993557371656522 1102 16 1.0345911814320559 1102 17 1.0176451928055883 1102 18 1.1491633124131866 1102 19 1.0540794009260566 1102 20 1.1346610028435578 1102 21 1.1072774415475735 1102 22 0.9229486684709443 1102 23 1.055310221459024 1102 24 1.1346979700903788 1102 25 1.2541114400278914 1103 1 1.3501617835394768 1103 2 4.069815135581241 1103 3 2.413752870887393 1103 4 1.6042154462060125 1103 5 1.2317029153611614 1103 6 0.9361725556022478 1103 7 0.7050569006801197 1103 8 0.6071155411791409 1103 9 0.734744464114911 1103 10 0.6853084527552866 1103 11 0.6062763872168588 1103 12 0.604878482541821 1103 13 0.6942155815571098 1103 14 0.8001204857818962 1103 15 0.9043408037924721 1103 16 0.795869349007809 1103 17 0.7589010198382127 1103 18 0.7697354217135484 1103 19 0.904929758179108 1103 20 0.9010882436921932 1103 21 1.0423067201988232 1103 22 0.9444639927682772 1103 23 1.07556544295403 1103 24 1.149499817392602 1103 25 1.0589442383400183 1104 1 1.5074465513829622 1104 2 3.9958511092873685 1104 3 2.3305709913060895 1104 4 1.6381039974595382 1104 5 1.2419580052420187 1104 6 1.013621038145807 1104 7 0.7526262487699142 1104 8 0.8630924473656449 1104 9 0.9528276304170262 1104 10 0.8629983637434293 1104 11 0.6970689708305713 1104 12 0.9213927533907551 1104 13 0.7920215847113212 1104 14 0.9030459113415361 1104 15 1.0060193645949838 1104 16 0.9942009580641908 1104 17 0.999436653176744 1104 18 1.0502154568563071 1104 19 1.1820633088996644 1104 20 1.1207576409400133 1104 21 0.8935045493233886 1104 22 0.9171745005023766 1104 23 1.1139071462073389 1104 24 1.1411596429561612 1104 25 1.0474086808859937 1105 1 1.2275930599147955 1105 2 4.095021599850142 1105 3 2.2969694103576828 1105 4 1.3397895397257855 1105 5 0.9955145606975435 1105 6 0.6580781848715773 1105 7 0.5554235453549978 1105 8 0.4651530878091492 1105 9 0.5794256219235407 1105 10 0.4158743949527661 1105 11 0.5089431376896982 1105 12 0.5316651473389697 1105 13 0.4706572711422723 1105 14 0.5098589094317632 1105 15 0.3576323424605974 1105 16 0.5121650927824604 1105 17 0.5756650525736049 1105 18 0.4950279484740179 1105 19 0.5627074031172654 1105 20 0.47397016358023336 1105 21 0.4538902568573633 1105 22 0.509833905444296 1105 23 0.25632876187182063 1105 24 0.23696093433254362 1105 25 0.4514694164537758 1106 1 0.4937790358523806 1106 2 2.381379347766572 1106 3 1.2412002682105658 1106 4 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-0.8324637332184537 2315 14 -0.8901485107344627 2315 15 -1.2658265130612136 2315 16 -1.1520291012122925 2315 17 -0.9581794935698653 2315 18 -1.0287839145854392 2315 19 -0.9326123957412662 2315 20 -0.9988393930442214 2315 21 -1.0676065830449986 2315 22 -1.2610615918202015 2315 23 -1.1484962699215302 2315 24 -1.190922951058866 2315 25 -1.0828808002769108 2316 1 -0.4079283937738296 2316 2 0.22478177565050572 2316 3 0.1342740182458826 2316 4 -0.3078615520550514 2316 5 -0.5785141242754364 2316 6 -0.5805098350594022 2316 7 -0.7398416128566581 2316 8 -0.800732955501843 2316 9 -0.7047626189587106 2316 10 -0.8957509645433746 2316 11 -1.0464808092392985 2316 12 -0.9730911091112802 2316 13 -1.0466392378675664 2316 14 -1.1458730483820219 2316 15 -1.0288362597090241 2316 16 -1.0826124649187179 2316 17 -1.377564258427995 2316 18 -1.2090080815278554 2316 19 -1.1264280916514835 2316 20 -1.071435590892996 2316 21 -1.1227151923401877 2316 22 -1.1744809554638067 2316 23 -1.1705498410116206 2316 24 -1.2508824090765813 2316 25 -1.22401006182902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67875.0 3 3.0 4 595.0 5 2756.0 6 3294.0 7 2921.0 8 2830.0 9 2773.0 10 2693.0 11 2673.0 12 2684.0 13 2606.0 14 2600.0 15 2791.0 16 2852.0 17 3180.0 18 3533.0 19 4635.0 20 5843.0 21 7542.0 22 9155.0 23 11170.0 24 12758.0 25 14689.0 26 16617.0 27 19067.0 28 22386.0 29 27260.0 30 34104.0 31 44096.0 32 56645.0 33 76668.0 34 106243.0 35 156358.0 36 255086.0 37 353374.0 38 876960.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.818887809977404 18.896956943921886 13.013429819561443 25.27072542653927 2 17.13470482654727 21.648293287112875 38.68028079489484 22.536721091445013 3 18.3567452119235 24.76286601946997 29.936714707015454 26.94367406159108 4 12.247247461491497 17.08459592261576 38.66868831148349 31.999468304409245 5 13.268283231537659 37.543656488601215 36.005569286018435 13.182490993842693 6 31.269603458724877 37.46746180690889 18.420638800711032 12.842295933655205 7 27.654614148960377 31.714740809664242 22.73187898067647 17.898766060698907 8 24.941096636661886 36.789222610780904 20.871008280490404 17.398672472066806 9 26.18001500462981 15.727555574580446 20.55129623329721 37.541133187492534 10 14.653588745706442 29.307537963324688 34.67978604189675 21.35908724907212 11 33.954861727542834 22.81867279708955 24.647841630341627 18.578623845025984 12 22.792348089387943 24.9060633573873 31.98892451049085 20.312664042733907 13 28.51366299961925 22.06338442267096 26.0531740649705 23.36977851273929 14 20.876054887255652 21.969816785644927 28.027231850577184 29.12689647652224 15 22.79243820728468 30.576641891754893 23.735206584013536 22.895713316946896 16 22.24555775092765 28.326172715454994 27.48059649035851 21.947673043258845 17 21.723459716173686 28.47351547662229 27.849674336450665 21.953350470753364 18 21.530279497158347 27.91569620606793 29.406837359590178 21.147186937183545 19 23.226738160681297 27.165998287748387 27.713242914432477 21.89402063713784 20 23.22964926873536 27.482870771313195 28.211747275950227 21.075732684001213 21 23.049141738620275 26.857268253205806 27.34581079948487 22.747779208689046 22 22.945421365085444 27.78903020130448 27.605366617476427 21.660181816133647 23 22.455274104941385 27.573338572231915 27.735507115770048 22.235880207056653 24 22.77014428782948 27.05151231416555 28.293788080460896 21.88455531754407 25 22.72674430655349 27.331742445611372 27.976448493357836 21.9650647544773 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 224.0 1 224.0 2 397.0 3 570.0 4 570.0 5 570.0 6 1416.0 7 2262.0 8 2262.0 9 2262.0 10 3518.0 11 4774.0 12 4774.0 13 4774.0 14 8064.0 15 11354.0 16 11354.0 17 11354.0 18 19694.5 19 28035.0 20 28035.0 21 28035.0 22 43374.5 23 58714.0 24 58714.0 25 58714.0 26 82551.0 27 106388.0 28 106388.0 29 106388.0 30 136523.0 31 166658.0 32 166658.0 33 166658.0 34 189164.5 35 211671.0 36 211671.0 37 211671.0 38 229339.5 39 247008.0 40 247008.0 41 247008.0 42 262002.0 43 276996.0 44 276996.0 45 276996.0 46 281823.0 47 286650.0 48 286650.0 49 286650.0 50 278554.5 51 270459.0 52 270459.0 53 270459.0 54 241780.5 55 213102.0 56 213102.0 57 213102.0 58 182629.5 59 152157.0 60 152157.0 61 152157.0 62 123589.5 63 95022.0 64 95022.0 65 95022.0 66 72973.0 67 50924.0 68 50924.0 69 50924.0 70 36956.0 71 22988.0 72 22988.0 73 22988.0 74 15935.5 75 8883.0 76 8883.0 77 8883.0 78 5982.5 79 3082.0 80 3082.0 81 3082.0 82 2015.0 83 948.0 84 948.0 85 948.0 86 618.5 87 289.0 88 289.0 89 289.0 90 201.0 91 113.0 92 113.0 93 113.0 94 69.0 95 25.0 96 25.0 97 25.0 98 22.0 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.448856967127244 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 9.011789673840802E-5 9 0.0 10 9.011789673840802E-5 11 4.505894836920401E-5 12 0.0 13 0.0 14 9.011789673840802E-5 15 0.0 16 0.0 17 0.0 18 3.604715869536321E-4 19 6.758842255380602E-4 20 4.055305353228361E-4 21 0.002748595850521445 22 0.0018023579347681605 23 8.561200190148762E-4 24 4.505894836920401E-5 25 4.055305353228361E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2219315.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.90744386600646 #Duplication Level Percentage of deduplicated Percentage of total 1 84.10527830192095 63.001114132359945 2 11.076428799702805 16.594139370991105 3 2.705094884478502 6.078952296338839 4 0.9109299504824978 2.7294173652652702 5 0.40535896864664756 1.5182202094740513 6 0.2234723400084589 1.0043845058873246 7 0.13184477477280718 0.6913308545724223 8 0.08717931787631573 0.5224303888077484 9 0.06600537832291842 0.4449864754420678 >10 0.26275757777007924 3.4068222851987304 >50 0.014202796637633668 0.7570108150824668 >100 0.00943974338519947 1.4795929684399465 >500 0.0012164642394671614 0.655032126869292 >1k 7.907017556532131E-4 1.1165662052708514 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3863 0.17406271755023509 No Hit GTCCTACAGTGGACATTTCTAAATT 2627 0.11836985736589895 No Hit GGTATCAACGCAGAGTACTTTTTTT 2344 0.10561817497741421 No Hit CTGTAGGACGTGGAATATGGCAAGA 2274 0.10246404859156992 No Hit TATCAACGCAGAGTACTTTTTTTTT 2248 0.10129251593397062 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2231 0.10052651381169414 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.011789673840802E-5 0.0 0.0 0.0 0.0 6 9.011789673840802E-5 0.0 0.0 0.0 0.0 7 9.011789673840802E-5 0.0 0.0 0.0 0.0 8 9.011789673840802E-5 0.0 0.0 0.0 0.0 9 9.011789673840802E-5 0.0 0.0 9.011789673840802E-5 0.0 10 9.011789673840802E-5 0.0 0.0 9.011789673840802E-5 0.0 11 1.3517684510761205E-4 0.0 0.0 2.703536902152241E-4 0.0 12 1.3517684510761205E-4 0.0 0.0 2.703536902152241E-4 1.8023579347681604E-4 13 1.3517684510761205E-4 0.0 0.0 2.703536902152241E-4 1.8023579347681604E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCTAC 45 0.009349626 13.071184 1 TAGGACC 580 0.0 12.566216 4 CGGTAGG 120 8.258212E-8 11.437286 1 GGTATCA 970 0.0 11.117244 1 TCGAACT 130 3.446985E-8 10.921933 19 GTATCAA 1915 0.0 10.852837 1 GTAGGAC 1530 0.0 10.764644 3 GTCAACG 75 0.0020396982 10.456947 2 TAGGACA 645 0.0 10.419346 4 GGCGAGG 635 0.0 10.136471 19 GTGGTAT 340 0.0 10.0917225 1 CTACACT 330 0.0 10.039126 4 CGTGCGC 95 1.7060922E-4 9.963644 10 AGGACCT 970 0.0 9.953373 5 TGGCGAG 1285 0.0 9.944484 18 TCCAACG 200 2.7284841E-11 9.93896 18 GTCCTAA 760 0.0 9.93238 1 GCGTGCG 105 4.2739826E-5 9.916199 9 GTGCGCC 105 4.2739826E-5 9.916199 11 TCTATAC 230 0.0 9.877004 3 >>END_MODULE