FastQCFastQC Report
Thu 26 May 2016
SRR1512044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219315
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50730.22858404507697197No Hit
TATCAACGCAGAGTACTTTTTTTTT31270.14089933155050094No Hit
GGTATCAACGCAGAGTACTTTTTTT30140.1358076703847809No Hit
GTCCTACAGTGGACATTTCTAAATT29730.13396025350164353No Hit
CTGTAGGACGTGGAATATGGCAAGA26960.12147892480337402No Hit
CTTTAGGACGTGAAATATGGCGAGG24310.10953830348553494No Hit
GTCCTAAAGTGTGTATTTCTCATTT23160.1043565244230765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.012.39481451
TAGGACC6100.011.2174274
GGCGAGG8150.010.9536119
AGGACGT15950.010.6059585
GTATCAA24150.010.515931
TGTAGGA18200.010.4957922
GTAGGAC17550.010.4513113
GTATTAG1855.4569682E-1110.3214681
GGACGTG15850.010.309166
GACGTGG7850.010.2813067
TAGGACG17100.010.0594224
AGTGCGC856.6267414E-410.0536238
CTGTAGG18200.010.019481
TTAGGAC10650.09.9052153
GTGGTAT4450.09.8691791
TCCAACG1651.0308213E-89.78303918
CGTGGAA8000.09.7324669
GTATAGG2950.09.7091771
GTCCTAA9300.09.5473581
GTCCTAC15950.09.5174281
Produced by FastQC (version 0.11.5)