FastQCFastQC Report
Thu 26 May 2016
SRR1512043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769252
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34470.19482809684544655No Hit
TATCAACGCAGAGTACTTTTTTTTT22640.12796368182712242No Hit
GGTATCAACGCAGAGTACTTTTTTT20750.11728120132123632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCC400.005411145314.1971819
GGTATCA9850.013.8418541
GCCTAAT603.1237255E-413.0783931
TGGACCG907.776933E-611.5680735
CGTGCCC752.1468918E-411.35774510
GTCTAAT1251.4408761E-710.9858491
GTTCTAA1707.6397555E-1110.9627711
TAGACCT1303.4497134E-810.9209084
GGCGAGG4550.010.60948219
GCGTAGA650.00839725110.5633171
TAGGACC3700.010.4880084
GACGTGG6050.010.4816657
AGCACCG1002.4992125E-510.4112665
TTAGGAC6700.010.3123813
GTCTATA1251.7063048E-610.2011461
GGACGTG10900.010.1594516
TAAGGCC1401.02279955E-710.1408434
AGGACGT10950.010.113065
GACCGTG856.822847E-410.021547
GCACCGA856.822847E-410.021546
Produced by FastQC (version 0.11.5)