FastQCFastQC Report
Thu 26 May 2016
SRR1512043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769252
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43470.24569705163538036No Hit
TATCAACGCAGAGTACTTTTTTTTT28770.16261109214515512No Hit
GGTATCAACGCAGAGTACTTTTTTT28500.16108502350145712No Hit
GTCCTACAGTGGACATTTCTAAATT18510.10462048368463056No Hit
GTCCTAAAGTGTGTATTTCTCATTT18290.10337702034532106No Hit
CTGTAGGACGTGGAATATGGCAAGA17900.1011726989710906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9950.013.0549651
ACCGTGC600.005900066311.07738
GACGTGA4850.010.9631017
TTAGGAC7200.010.8239553
GTATCAA21850.010.7533441
GTCCTAA6550.010.64489651
TAGGACC3400.010.6220734
TGTTAGG1002.387877E-510.4543562
GGACGTG9500.010.299056
AGGACGT10150.010.1125895
GTGGTAT3500.010.0970281
GGCGAGG5300.010.03312219
TAGGACG10800.09.9439584
GTCCTAG1752.3446773E-99.8241351
GTGTTAG1201.5953414E-59.5512431
TGGTATC3100.09.5039612
CCTATAC1606.5905624E-89.503963
GTAGGAC9950.09.45620253
GTCCTAC9750.09.40431
GTTTTAG1856.1027094E-99.2931011
Produced by FastQC (version 0.11.5)