FastQCFastQC Report
Thu 26 May 2016
SRR1512042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851354
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20880.24525637983729445No Hit
GTCCTACAGTGGACATTTCTAAATT20760.24384686041294218No Hit
CTGTAGGACGTGGAATATGGCAAGA20180.23703418319523956No Hit
GTCCTAAAGTGTGTATTTCTCATTT19880.23351038463435891No Hit
CTTTAGGACGTGAAATATGGCGAGG17800.20907871461225297No Hit
TATCAACGCAGAGTACTTTTTTTTT12510.14694239998872385No Hit
GGTATCAACGCAGAGTACTTTTTTT12160.1428313016676964No Hit
GTCCTACAGTGTGCATTTCTCATTT11240.1320249860809957No Hit
CTGTAGGACCTGGAATATGGCGAGA9150.10747585610686036No Hit
CTGAAGGACCTGGAATATGGCGAGA8860.10406951749800904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG400.00437138414.659621
CGGTAGG400.00437138414.659621
GTCGAGG400.005392637614.2033119
CCAACGT400.005392637614.2033119
CATACCC751.0021959E-613.8876814
TCCAACG751.0021959E-613.88768118
GTACTAG500.00121014313.6823121
TATGTCG500.001536093713.25642216
CCTACAC957.694325E-812.9574053
CTTAGGC604.2034074E-412.6251643
TGGTATC1352.3101165E-1012.3068412
TAGGACC4000.011.8360914
TGTAGGA12500.011.7276962
ATAAGGG658.240633E-411.6539973
TAAGGGT658.240633E-411.6539974
AGGACGT13050.011.391675
TTGGGAC752.1358245E-411.3626483
GGACGTG12900.011.30392556
CTGTAGG12850.011.2561541
ACTGTCC1105.172278E-711.1904868
Produced by FastQC (version 0.11.5)