##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512042_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 851354 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.251628582234886 31.0 30.0 34.0 25.0 34.0 2 27.402030177810875 31.0 27.0 34.0 10.0 34.0 3 29.35939573902278 31.0 28.0 34.0 16.0 34.0 4 33.85760095095577 37.0 35.0 37.0 28.0 37.0 5 34.30480622631714 37.0 35.0 37.0 32.0 37.0 6 34.595066212174956 37.0 35.0 37.0 32.0 37.0 7 34.68771627313667 37.0 35.0 37.0 32.0 37.0 8 34.72575567860138 37.0 35.0 37.0 32.0 37.0 9 36.44065570843621 39.0 37.0 39.0 33.0 39.0 10 36.35360378878821 39.0 37.0 39.0 32.0 39.0 11 36.37380807513678 39.0 37.0 39.0 32.0 39.0 12 36.29797358090759 39.0 37.0 39.0 32.0 39.0 13 36.303840705511455 39.0 37.0 39.0 32.0 39.0 14 37.60103082853901 40.0 38.0 41.0 33.0 41.0 15 37.55717715544885 40.0 38.0 41.0 32.0 41.0 16 37.518342546108904 40.0 38.0 41.0 32.0 41.0 17 37.501986247788814 40.0 38.0 41.0 32.0 41.0 18 37.468785017748196 40.0 38.0 41.0 32.0 41.0 19 37.5020203111749 40.0 38.0 41.0 32.0 41.0 20 37.428112160158996 40.0 38.0 41.0 32.0 41.0 21 37.379029170004486 40.0 38.0 41.0 32.0 41.0 22 37.29350305513335 40.0 38.0 41.0 32.0 41.0 23 37.215396885431915 40.0 38.0 41.0 32.0 41.0 24 37.16537069186261 40.0 38.0 41.0 32.0 41.0 25 37.0776292822962 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8376650489648867 1101 2 3.8048374919936414 1101 3 1.8416757484925057 1101 4 0.9831393442523719 1101 5 0.5429668233044893 1101 6 0.04972967937355577 1101 7 0.07499028177878131 1101 8 -0.034368157253375387 1101 9 -0.07798803252556752 1101 10 0.004629547689049218 1101 11 0.07635168331402298 1101 12 0.10310542134390488 1101 13 0.08247207723186278 1101 14 -0.06861160250674914 1101 15 0.03642181217758633 1101 16 0.029736813961605435 1101 17 0.016448024692024887 1101 18 0.12118740230457803 1101 19 -0.033835217480401525 1101 20 0.05812936265947144 1101 21 0.09878725727580928 1101 22 0.23008571738432693 1101 23 0.17809473101158346 1101 24 0.1559792632991659 1101 25 0.07345600743343539 1102 1 1.5291393521007777 1102 2 4.311107454246837 1102 3 2.4651785795029575 1102 4 1.5636053721269363 1102 5 0.9793235306215635 1102 6 0.8763121416267481 1102 7 0.6335976424059595 1102 8 0.7095433555224702 1102 9 0.8135080476138015 1102 10 0.8915252358423587 1102 11 0.8214518575300502 1102 12 0.870953490449601 1102 13 0.8238973821099123 1102 14 0.9383025960995255 1102 15 0.6860207523634116 1102 16 0.6188319794668331 1102 17 0.6415932047152495 1102 18 0.7726283140351242 1102 19 0.8029007726473552 1102 20 0.9048192581298551 1102 21 0.8828410464744181 1102 22 0.8229555634935082 1102 23 0.8796136770046203 1102 24 0.8750468445999786 1102 25 0.6821867710803602 1103 1 0.9639472828318603 1103 2 3.9181240815864626 1103 3 2.1319356483719396 1103 4 1.2267842635043138 1103 5 0.8279202551456422 1103 6 0.6045456242695266 1103 7 0.553934138566575 1103 8 0.3391538678405013 1103 9 0.3047019565883673 1103 10 0.24497743349073176 1103 11 0.2844627757831333 1103 12 0.3809406425052728 1103 13 0.3584006115931899 1103 14 0.5279407539942653 1103 15 0.5389430347982227 1103 16 0.4613009344340355 1103 17 0.5019755435410431 1103 18 0.4642401561403844 1103 19 0.4488361729832988 1103 20 0.5093625312061647 1103 21 0.2979358813008446 1103 22 0.4458196552388287 1103 23 0.700633563822727 1103 24 0.493176693824104 1103 25 0.3889424401733024 1104 1 1.2685487632426558 1104 2 3.9337798662846275 1104 3 2.250000771635225 1104 4 1.5176746423663872 1104 5 1.1196278350685418 1104 6 0.835619439232218 1104 7 0.6044639785356267 1104 8 0.5987875830025189 1104 9 0.71319905999367 1104 10 0.75242353971921 1104 11 0.5625792805631207 1104 12 0.7417068195748655 1104 13 0.6494071862311017 1104 14 0.7841371171941631 1104 15 0.7135891165106685 1104 16 0.8275627886377066 1104 17 0.9278368926123051 1104 18 0.6105523448218975 1104 19 0.8122454847340137 1104 20 0.6373489860985799 1104 21 0.6768030618678011 1104 22 0.8678567587207411 1104 23 0.7303337148772329 1104 24 0.7734919802083837 1104 25 0.7363575949116026 1105 1 1.5988692804306197 1105 2 4.445490255422548 1105 3 2.6142678551512084 1105 4 1.6737026100074317 1105 5 1.386753773011641 1105 6 1.0273127053246043 1105 7 0.851840565984638 1105 8 0.7216364361312628 1105 9 0.7750734164332584 1105 10 0.8282765746247733 1105 11 0.8298016320509092 1105 12 0.8396745506042151 1105 13 0.8959764709325881 1105 14 0.8364318238334576 1105 15 0.773933711374525 1105 16 0.8566835356755576 1105 17 0.9560963453176043 1105 18 0.7126825699539552 1105 19 0.8631724045526568 1105 20 0.7803225597887362 1105 21 0.7126483322692323 1105 22 0.858640909362407 1105 23 0.6750051953650598 1105 24 0.7442349686940375 1105 25 0.8089042879912967 1106 1 0.059308117583849906 1106 2 1.9519711516043898 1106 3 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16 -0.776660991657792 2315 17 -0.5506560354063268 2315 18 -0.42576855357717136 2315 19 -0.464626561868009 2315 20 -0.49909833727710406 2315 21 -0.5978585693648242 2315 22 -1.020274658559856 2315 23 -1.0141853984766414 2315 24 -0.7693590782301456 2315 25 -0.8916348896761406 2316 1 -0.5778044947150569 2316 2 -0.017267653326314303 2316 3 -0.19252096868981283 2316 4 -0.47878920618631327 2316 5 -0.893530385041089 2316 6 -0.8635974969274685 2316 7 -0.88576283521882 2316 8 -1.0792179396582497 2316 9 -1.1166178129713913 2316 10 -1.171219493073302 2316 11 -1.2227499496988656 2316 12 -1.2528927875194213 2316 13 -1.4244755740002475 2316 14 -1.5688886689607173 2316 15 -1.2169778491856391 2316 16 -1.3665353828395368 2316 17 -1.781212303030017 2316 18 -1.6810990956034289 2316 19 -1.3982475707261415 2316 20 -1.424268521560336 2316 21 -1.4304684414773874 2316 22 -1.5399505251467716 2316 23 -1.561643315250116 2316 24 -1.5493157844878027 2316 25 -1.4998350662690356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19673.0 3 0.0 4 125.0 5 747.0 6 901.0 7 850.0 8 770.0 9 681.0 10 706.0 11 668.0 12 653.0 13 611.0 14 632.0 15 718.0 16 757.0 17 756.0 18 888.0 19 1151.0 20 1482.0 21 1717.0 22 2189.0 23 2568.0 24 3015.0 25 3495.0 26 3987.0 27 4704.0 28 5648.0 29 7051.0 30 9159.0 31 12032.0 32 16890.0 33 24207.0 34 36690.0 35 58208.0 36 99476.0 37 151233.0 38 376316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.18680830770749 19.40544121481781 13.547948327017902 26.8598021504568 2 15.455696079762573 21.414248443349564 41.022103466335615 22.10795201055225 3 17.2667304082673 24.616669446552198 30.725291711790863 27.39130843338964 4 11.26805065812811 17.315476288359484 39.74880014659002 31.667672906922384 5 12.12503846813429 37.66177171893243 36.96499928349429 13.248190529438988 6 29.333626200147062 38.610613211425566 19.052121679113508 13.00363890931387 7 26.087385505911758 31.778790021542154 23.748757861007288 18.385066611538793 8 22.832570235178316 38.38485518362514 21.704719775792444 17.077854805404098 9 24.847008412481763 15.77252235850187 21.65256755709141 37.727901671924954 10 13.755030222445658 29.297096155065933 35.750933219318874 21.19694040316954 11 31.945818073327896 22.72615151863972 26.60397437493687 18.724056033095515 12 22.244330795415305 24.7663134254376 33.26360127514524 19.72575450400186 13 27.486920834341532 22.65649776708631 26.887992538943845 22.968588859628312 14 19.071854951054437 23.03013787449169 28.88328474406651 29.01472243038736 15 21.088172487590356 32.71600297878438 24.18018826481111 22.01563626881415 16 20.045950333233883 28.617120492767988 30.0224113588472 21.314517815150925 17 19.8171383466807 29.473403543061995 29.400225992947703 21.309232117309605 18 19.600751747224994 27.751805955247548 32.27650202619369 20.370940271333765 19 22.03308404211209 27.084373351876494 29.009675255800516 21.8728673502109 20 21.92019282271023 27.795318505154153 29.88293913680828 20.401549535327337 21 20.93667665497123 27.206168238131205 28.560051401984428 23.29710370491314 22 21.401068438313427 28.88460020485449 29.101905710553766 20.61242564627832 23 20.09662335099554 28.71602296126021 29.680964401119635 21.506389286624607 24 21.51857102752912 27.00395723043203 30.584610613928653 20.892861128110198 25 20.87059274024462 27.93336708361181 29.992447298470314 21.203592877673252 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 105.0 1 105.0 2 209.5 3 314.0 4 314.0 5 314.0 6 707.5 7 1101.0 8 1101.0 9 1101.0 10 1632.0 11 2163.0 12 2163.0 13 2163.0 14 3615.0 15 5067.0 16 5067.0 17 5067.0 18 9089.5 19 13112.0 20 13112.0 21 13112.0 22 20278.0 23 27444.0 24 27444.0 25 27444.0 26 39046.0 27 50648.0 28 50648.0 29 50648.0 30 64303.0 31 77958.0 32 77958.0 33 77958.0 34 86277.0 35 94596.0 36 94596.0 37 94596.0 38 99355.5 39 104115.0 40 104115.0 41 104115.0 42 106307.5 43 108500.0 44 108500.0 45 108500.0 46 107901.0 47 107302.0 48 107302.0 49 107302.0 50 100648.5 51 93995.0 52 93995.0 53 93995.0 54 80785.0 55 67575.0 56 67575.0 57 67575.0 58 56760.0 59 45945.0 60 45945.0 61 45945.0 62 36596.0 63 27247.0 64 27247.0 65 27247.0 66 20811.0 67 14375.0 68 14375.0 69 14375.0 70 10268.5 71 6162.0 72 6162.0 73 6162.0 74 4290.5 75 2419.0 76 2419.0 77 2419.0 78 1632.0 79 845.0 80 845.0 81 845.0 82 543.5 83 242.0 84 242.0 85 242.0 86 155.0 87 68.0 88 68.0 89 68.0 90 51.0 91 34.0 92 34.0 93 34.0 94 24.5 95 15.0 96 15.0 97 15.0 98 11.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.1133934884900993 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.6983980811742237E-4 19 8.222196642054892E-4 20 2.3491990405871119E-4 21 0.00293649880073389 22 0.0021142791365284007 23 8.222196642054892E-4 24 1.1745995202935559E-4 25 3.5237985608806675E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 851354.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.71709354403413 #Duplication Level Percentage of deduplicated Percentage of total 1 89.20781106164507 70.22179608198037 2 7.849391574724858 12.3576258170274 3 1.573978146560605 3.716969549972298 4 0.5422688977192612 1.7074332619114945 5 0.2467353637501738 0.971114535447186 6 0.1337251870909647 0.6315874836859761 7 0.08603820709178306 0.4740874317203407 8 0.06337920950619286 0.3991221730756736 9 0.04227764320215592 0.29951758752888646 >10 0.21646176751418042 3.1101654843050635 >50 0.018892733184344604 1.0556146388994718 >100 0.0163201784073083 2.6440555481572208 >500 0.001511127557313308 0.8001872065869183 >1k 0.0012089020458506464 1.6107231997017186 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2088 0.24525637983729445 No Hit GTCCTACAGTGGACATTTCTAAATT 2076 0.24384686041294218 No Hit CTGTAGGACGTGGAATATGGCAAGA 2018 0.23703418319523956 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1988 0.23351038463435891 No Hit CTTTAGGACGTGAAATATGGCGAGG 1780 0.20907871461225297 No Hit TATCAACGCAGAGTACTTTTTTTTT 1251 0.14694239998872385 No Hit GGTATCAACGCAGAGTACTTTTTTT 1216 0.1428313016676964 No Hit GTCCTACAGTGTGCATTTCTCATTT 1124 0.1320249860809957 No Hit CTGTAGGACCTGGAATATGGCGAGA 915 0.10747585610686036 No Hit CTGAAGGACCTGGAATATGGCGAGA 886 0.10406951749800904 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.1745995202935559E-4 0.0 0.0 0.0 6 0.0 1.1745995202935559E-4 0.0 0.0 0.0 7 0.0 1.1745995202935559E-4 0.0 0.0 0.0 8 0.0 1.1745995202935559E-4 0.0 0.0 0.0 9 0.0 1.1745995202935559E-4 0.0 0.0 0.0 10 0.0 1.1745995202935559E-4 0.0 0.0 0.0 11 0.0 1.1745995202935559E-4 0.0 0.0 0.0 12 0.0 1.1745995202935559E-4 0.0 0.0 0.0 13 0.0 1.1745995202935559E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATAGG 40 0.004371384 14.65962 1 CGGTAGG 40 0.004371384 14.65962 1 GTCGAGG 40 0.0053926376 14.20331 19 CCAACGT 40 0.0053926376 14.20331 19 CATACCC 75 1.0021959E-6 13.887681 4 TCCAACG 75 1.0021959E-6 13.887681 18 GTACTAG 50 0.001210143 13.682312 1 TATGTCG 50 0.0015360937 13.256422 16 CCTACAC 95 7.694325E-8 12.957405 3 CTTAGGC 60 4.2034074E-4 12.625164 3 TGGTATC 135 2.3101165E-10 12.306841 2 TAGGACC 400 0.0 11.836091 4 TGTAGGA 1250 0.0 11.727696 2 ATAAGGG 65 8.240633E-4 11.653997 3 TAAGGGT 65 8.240633E-4 11.653997 4 AGGACGT 1305 0.0 11.39167 5 TTGGGAC 75 2.1358245E-4 11.362648 3 GGACGTG 1290 0.0 11.3039255 6 CTGTAGG 1285 0.0 11.256154 1 ACTGTCC 110 5.172278E-7 11.190486 8 >>END_MODULE