FastQCFastQC Report
Thu 26 May 2016
SRR1512042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851354
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32230.37857342539061306No Hit
TATCAACGCAGAGTACTTTTTTTTT23220.2727420086121637No Hit
GGTATCAACGCAGAGTACTTTTTTT22060.2591166541767584No Hit
CTGTAGGACGTGGAATATGGCAAGA20070.23574212372291667No Hit
GTCCTACAGTGGACATTTCTAAATT18120.21283743307719236No Hit
CTTTAGGACGTGAAATATGGCGAGG17850.20966601437239973No Hit
GTCCTAAAGTGTGTATTTCTCATTT16540.19427876065655417No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11460.13460910502564152No Hit
CTGAAGGACCTGGAATATGGCGAGA9890.11616789255703268No Hit
CTGTAGGACCTGGAATATGGCGAGA9830.11546313284485654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCC250.006047141718.9906028
GTGCGCC508.743383E-515.19248211
ATTACTC456.7350955E-414.7843613
GCGCCAC400.005293852614.24295313
TTACTCT903.6099664E-813.7283354
GGTATCA6800.013.3312621
TAGGACC3100.012.5701144
GGACGGG907.4416675E-611.6121886
CCAACTT855.3429292E-511.17094313
GTCACAA600.005680554511.1327721
CTAGATA600.005852518611.0882714
TCTAGAC600.005852518611.0882713
TAGTCTG600.005852518611.0882715
TGCGCCA600.005893621611.07785112
GTGTAGG1657.2759576E-1110.9881931
TGTAGGA12200.010.98442
CACCTTT3200.010.97894314
AAGGCGT700.00148904810.85815056
CTGTAGG11400.010.7979531
TAGGACT1252.220495E-710.644744
Produced by FastQC (version 0.11.5)