FastQCFastQC Report
Thu 26 May 2016
SRR1512041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2204073
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA34760.15770802509717236No Hit
GTCCTACAGTGGACATTTCTAAATT34640.15716357852031218No Hit
GTATCAACGCAGAGTACTTTTTTTT34530.15666450249152364No Hit
GTCCTAAAGTGTGTATTTCTCATTT31030.1407848106664344No Hit
CTTTAGGACGTGAAATATGGCGAGG27550.12499585993748846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.013.1721461
TAGGACC7450.012.197254
GGCGAGG8900.011.80618319
GCGTTAA600.004656913711.4348741
CGGTAGG1302.062552E-811.3092151
TTAGGCC803.9006738E-410.6487723
TGTAGGA20800.010.5081452
GTAGGAC21500.010.478173
TCGAACT1002.495808E-510.41284219
AATGCCG1659.895302E-1010.32678418
TAGGACT3300.010.3260814
CTGTAGG20700.010.3221641
AGGACGT21150.010.0250065
CGTGCGC856.81905E-410.02237310
GACGTGA11500.09.9594327
AGGACCG1054.2733875E-59.9163175
GTCCTAA10400.09.8955631
GGACGTG21100.09.869326
TGGCGAG19200.09.86064518
CTACACT3750.09.8441974
Produced by FastQC (version 0.11.5)