##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512041_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2204073 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.479938277906403 31.0 30.0 34.0 25.0 34.0 2 26.532716475361752 31.0 25.0 34.0 10.0 34.0 3 28.51015778515503 31.0 28.0 34.0 16.0 34.0 4 33.01298006009783 35.0 33.0 37.0 27.0 37.0 5 33.469386449541375 35.0 35.0 37.0 28.0 37.0 6 33.75439379730163 37.0 35.0 37.0 29.0 37.0 7 33.832373973094356 37.0 35.0 37.0 29.0 37.0 8 33.867953556892175 37.0 35.0 37.0 30.0 37.0 9 35.49375769314356 39.0 37.0 39.0 30.0 39.0 10 35.39961425960029 39.0 37.0 39.0 30.0 39.0 11 35.411689630969576 39.0 37.0 39.0 30.0 39.0 12 35.31462796377434 39.0 35.0 39.0 30.0 39.0 13 35.31415565636891 39.0 35.0 39.0 30.0 39.0 14 36.52236699964112 40.0 37.0 41.0 30.0 41.0 15 36.460426220002695 40.0 37.0 41.0 29.0 41.0 16 36.41105353588561 40.0 37.0 41.0 29.0 41.0 17 36.37240917156555 40.0 37.0 41.0 29.0 41.0 18 36.32378464778617 40.0 37.0 41.0 29.0 41.0 19 36.35461620372828 40.0 37.0 41.0 29.0 41.0 20 36.257955612178 40.0 37.0 41.0 28.0 41.0 21 36.20109814874553 40.0 37.0 41.0 28.0 41.0 22 36.100678607287506 40.0 37.0 41.0 28.0 41.0 23 36.00164332125116 40.0 37.0 41.0 27.0 41.0 24 35.91575415151858 40.0 37.0 41.0 27.0 41.0 25 35.816073242583165 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0448278580406019 1101 2 3.878560783103797 1101 3 2.1161307539673686 1101 4 1.190353733042187 1101 5 0.6704381334988412 1101 6 0.4489249867026075 1101 7 0.14515198844156174 1101 8 0.03695039309570802 1101 9 0.08606397647017872 1101 10 0.1438704365828869 1101 11 0.305512205202497 1101 12 0.04417259305549237 1101 13 0.12131973652807204 1101 14 0.12202287837730097 1101 15 0.17479309944774002 1101 16 0.371322947346826 1101 17 0.3384406198661054 1101 18 0.38107531612527623 1101 19 0.3770234758574915 1101 20 0.4174868884242926 1101 21 0.32980246084009224 1101 22 0.34938742547463875 1101 23 0.2875262108771608 1101 24 0.304005334345824 1101 25 0.23159382719629207 1102 1 1.5418936101070315 1102 2 4.288493303655539 1102 3 2.4918765722075804 1102 4 1.6720919103817167 1102 5 1.2190822163031143 1102 6 1.0361142468198992 1102 7 0.8305793991347201 1102 8 0.9222055658614039 1102 9 0.9803792744297866 1102 10 0.7497142198342388 1102 11 0.864394514621452 1102 12 0.8735785047734268 1102 13 0.8305382926009486 1102 14 0.970978520232471 1102 15 0.9894039582911773 1102 16 0.9631628316077396 1102 17 1.0404424918217359 1102 18 1.1602738469752794 1102 19 1.1177428631046809 1102 20 0.9417047516434636 1102 21 1.0733017672360035 1102 22 1.0295203833496345 1102 23 1.0754154283226711 1102 24 1.0459173304198188 1102 25 0.9756994431132142 1103 1 1.5488823950928818 1103 2 4.330191466115988 1103 3 2.63014922377992 1103 4 1.805073511652985 1103 5 1.3238770468285992 1103 6 1.233994035051495 1103 7 1.0110001067826246 1103 8 0.9665782984680646 1103 9 1.098883099862313 1103 10 0.940919249309438 1103 11 1.0748494491839296 1103 12 0.9962679692906491 1103 13 1.0203243707390328 1103 14 0.9859275784697488 1103 15 1.13595066629005 1103 16 0.9427285226380135 1103 17 1.041681582934551 1103 18 1.1283727195433357 1103 19 1.215571998808251 1103 20 1.1619905057900937 1103 21 1.2856085329457017 1103 22 1.2276497884060404 1103 23 1.3362579616685153 1103 24 1.4182826819517658 1103 25 1.471034257781838 1104 1 1.5473478075911693 1104 2 4.104954165206834 1104 3 2.4240670758522214 1104 4 1.6973762774814674 1104 5 1.208077890840059 1104 6 1.0018785145719278 1104 7 0.8329256394945403 1104 8 0.8699179576472673 1104 9 0.7786906590886389 1104 10 0.9381765971828031 1104 11 0.7909236872758214 1104 12 0.8677787666016314 1104 13 0.895097129255177 1104 14 0.8580571121255289 1104 15 0.9437477245033108 1104 16 0.9727943010099693 1104 17 0.8936091765449774 1104 18 0.9515336243687997 1104 19 0.879316686393075 1104 20 0.9883076428263067 1104 21 0.813917879645544 1104 22 0.9122730349210073 1104 23 1.0868617022343017 1104 24 0.9696327805271281 1104 25 1.0004888852225022 1105 1 1.2917283986938273 1105 2 4.201091529245925 1105 3 2.5166837719808974 1105 4 1.4244637676335472 1105 5 0.9799107394852911 1105 6 0.8757220930187515 1105 7 0.7759918949499038 1105 8 0.5363761816051422 1105 9 0.5196952279969977 1105 10 0.5090841983372343 1105 11 0.6278021268837009 1105 12 0.6299115324063251 1105 13 0.5767019205563244 1105 14 0.6184935982136324 1105 15 0.5107060125154277 1105 16 0.6465624255481686 1105 17 0.7442587262139071 1105 18 0.5630496283368558 1105 19 0.7715311527727593 1105 20 0.637676945609698 1105 21 0.6829828575440757 1105 22 0.7413271555709713 1105 23 0.6032625640228915 1105 24 0.6434813862945674 1105 25 0.8202025528535515 1106 1 0.5002961801746082 1106 2 2.5146798234612007 1106 3 1.3035605678397282 1106 4 0.5331911523383894 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0.6077836116487845 1107 17 0.5595958270182848 1107 18 0.3815073034251171 1107 19 0.4055522470364039 1107 20 0.6138750466225886 1107 21 0.6742503652447311 1107 22 0.5550550631839641 1107 23 0.6486132050502391 1107 24 0.5825847603822609 1107 25 0.5562144507336271 1108 1 1.3626681962199392 1108 2 3.479894636138777 1108 3 2.2183572161127714 1108 4 1.501433499348721 1108 5 1.0782780194516022 1108 6 0.7801187141047379 1108 7 0.655947928739046 1108 8 0.7203433413760578 1108 9 0.5931559658148586 1108 10 0.7369944392617924 1108 11 0.7771112998879701 1108 12 0.5119389598887665 1108 13 0.578610813236466 1108 14 0.6232341995464594 1108 15 0.8441183558709753 1108 16 0.930477197522869 1108 17 0.8510424515990564 1108 18 0.7152565383860221 1108 19 0.8580271604273051 1108 20 0.7847965064220475 1108 21 0.7630770765013466 1108 22 0.9783686882204279 1108 23 0.7675672988912225 1108 24 0.7556999995797185 1108 25 0.7833853981237198 1109 1 0.8939755277912163 1109 2 2.9955553525551757 1109 3 1.8568561910595314 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-1.121487551392562 2315 14 -1.30513903834958 2315 15 -1.5732736122242628 2315 16 -1.4864127175058286 2315 17 -1.349653812591022 2315 18 -1.275153461126088 2315 19 -1.316125133199769 2315 20 -1.272212170125151 2315 21 -1.2909317149357378 2315 22 -1.60904850843486 2315 23 -1.4791592049465692 2315 24 -1.42437494639843 2315 25 -1.372646242236307 2316 1 -0.5813196397422828 2316 2 0.03195638264717715 2316 3 -0.07092596798866069 2316 4 -0.41571300996551486 2316 5 -0.7088266378939707 2316 6 -0.851886996167508 2316 7 -0.9823124188440673 2316 8 -1.0620576544174156 2316 9 -1.1078655756844071 2316 10 -1.331680573421778 2316 11 -1.3680313883582826 2316 12 -1.3886794497973654 2316 13 -1.490609021580049 2316 14 -1.6100124436879852 2316 15 -1.4224835598430943 2316 16 -1.4446518854055768 2316 17 -1.494420791951363 2316 18 -1.4411704888139454 2316 19 -1.2403909357118579 2316 20 -1.3524873967958442 2316 21 -1.4277214322461305 2316 22 -1.451718717947827 2316 23 -1.6030897023626594 2316 24 -1.6150852847379085 2316 25 -1.4886510269533346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72702.0 3 1.0 4 609.0 5 2869.0 6 3422.0 7 3188.0 8 2867.0 9 2851.0 10 2757.0 11 2702.0 12 2686.0 13 2716.0 14 2815.0 15 2864.0 16 3026.0 17 3268.0 18 3887.0 19 4590.0 20 5995.0 21 7714.0 22 9626.0 23 11476.0 24 13476.0 25 15068.0 26 17093.0 27 19795.0 28 22712.0 29 27491.0 30 34447.0 31 43841.0 32 56545.0 33 75676.0 34 104495.0 35 152644.0 36 250206.0 37 346058.0 38 869895.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37128262085693 19.197186300090788 13.134909778396633 25.296621300655648 2 16.85476661058825 21.694031712414972 39.213282926964474 22.2379187500323 3 18.28315123863865 24.802808255443445 29.91602365257412 26.99801685334379 4 12.110624285130301 17.305960374270725 39.012954652590906 31.57046068800807 5 13.073387315211429 37.74221634219919 36.114547930127536 13.069848412461837 6 30.964837339631977 37.81216666795217 18.61106107743353 12.61193491498232 7 27.68642417923544 31.65380638481575 22.785452205984104 17.874317229964706 8 24.508908964861405 37.44991089220316 20.989922289290007 17.051257853645435 9 26.011116691688525 15.900426165558038 20.851487223880515 37.23696991887292 10 14.57225535281969 29.418231346344403 34.951262935149124 21.058250365686785 11 33.489181165959565 22.86566733497484 25.19299496885992 18.45215653020567 12 22.937715765312674 24.931615241418957 32.2420809111132 19.888588082155174 13 28.46330407386688 22.40978406795056 26.122682869396797 23.004228988785762 14 20.541706441531858 22.607428432465003 28.12662199782947 28.724243128173672 15 22.39463030489462 31.360258938791958 23.7171817811842 22.527928975129228 16 21.707039648868253 28.572238759786995 28.244209697228722 21.47651189411603 17 21.42669503233332 28.93542999710082 28.230598532807218 21.40727643775864 18 21.153634132630632 27.94037914558626 30.179382512552966 20.726604209230143 19 23.086143831060706 27.42196439476638 27.874311837528776 21.617579936644134 20 23.117201891055597 27.75881781802673 28.537789352376976 20.586190938540692 21 22.58189655172414 27.20961887477314 27.492921960072596 22.715562613430127 22 22.674619346226947 28.497978935925307 27.82022261876729 21.00717909908046 23 21.950147206346138 28.212485294110973 28.083722276591853 21.753645222951032 24 22.561342170919175 27.27947511672718 28.877200416864973 21.281982295488667 25 22.3989865993002 27.724058829507676 28.333523890413346 21.54343068077878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 203.0 1 203.0 2 378.0 3 553.0 4 553.0 5 553.0 6 1288.0 7 2023.0 8 2023.0 9 2023.0 10 3185.5 11 4348.0 12 4348.0 13 4348.0 14 7809.0 15 11270.0 16 11270.0 17 11270.0 18 20029.5 19 28789.0 20 28789.0 21 28789.0 22 45054.5 23 61320.0 24 61320.0 25 61320.0 26 86776.0 27 112232.0 28 112232.0 29 112232.0 30 144240.0 31 176248.0 32 176248.0 33 176248.0 34 199533.0 35 222818.0 36 222818.0 37 222818.0 38 240013.5 39 257209.0 40 257209.0 41 257209.0 42 268404.0 43 279599.0 44 279599.0 45 279599.0 46 282619.0 47 285639.0 48 285639.0 49 285639.0 50 273144.5 51 260650.0 52 260650.0 53 260650.0 54 229642.0 55 198634.0 56 198634.0 57 198634.0 58 169364.0 59 140094.0 60 140094.0 61 140094.0 62 112836.0 63 85578.0 64 85578.0 65 85578.0 66 65533.0 67 45488.0 68 45488.0 69 45488.0 70 32612.5 71 19737.0 72 19737.0 73 19737.0 74 13698.5 75 7660.0 76 7660.0 77 7660.0 78 5202.0 79 2744.0 80 2744.0 81 2744.0 82 1788.0 83 832.0 84 832.0 85 832.0 86 551.0 87 270.0 88 270.0 89 270.0 90 181.0 91 92.0 92 92.0 93 92.0 94 62.0 95 32.0 96 32.0 97 32.0 98 21.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.425748602700546 3 0.0 4 0.0 5 0.0 6 9.074109614336729E-5 7 0.0 8 4.5370548071683646E-5 9 0.0 10 4.5370548071683646E-5 11 0.0 12 0.0 13 0.0 14 4.5370548071683646E-5 15 0.0 16 0.0 17 0.0 18 5.444465768602039E-4 19 6.805582210752547E-4 20 5.898171249318875E-4 21 0.0033120500092329065 22 0.0016333397305806113 23 0.0010888931537204078 24 9.074109614336729E-5 25 4.0833493264515284E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2204073.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.20536347005732 #Duplication Level Percentage of deduplicated Percentage of total 1 83.41154591770757 61.89584082422866 2 11.662171983787617 17.30791421814559 3 2.8459752121226516 6.3355987512700445 4 0.9308007988422603 2.762816463852385 5 0.4163852797639183 1.5449010514231531 6 0.21060552413858644 0.9376835680503441 7 0.12691656079479569 0.6592522666903208 8 0.07723000572425638 0.4584704516450442 9 0.05181656142496285 0.3460560096877128 >10 0.2366490086972819 2.9988872167480123 >50 0.016495129147760506 0.8428038681574623 >100 0.010815246038012635 1.6177704893509484 >500 0.0015433165538640613 0.774858327197757 >1k 0.0010494552566265726 1.517146493552697 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 3476 0.15770802509717236 No Hit GTCCTACAGTGGACATTTCTAAATT 3464 0.15716357852031218 No Hit GTATCAACGCAGAGTACTTTTTTTT 3453 0.15666450249152364 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3103 0.1407848106664344 No Hit CTTTAGGACGTGAAATATGGCGAGG 2755 0.12499585993748846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.537054807168365E-5 7 0.0 0.0 0.0 4.537054807168365E-5 4.537054807168365E-5 8 0.0 0.0 0.0 4.537054807168365E-5 4.537054807168365E-5 9 0.0 0.0 0.0 4.537054807168365E-5 4.537054807168365E-5 10 0.0 0.0 0.0 4.537054807168365E-5 4.537054807168365E-5 11 0.0 0.0 0.0 4.537054807168365E-5 4.537054807168365E-5 12 0.0 0.0 0.0 4.537054807168365E-5 4.537054807168365E-5 13 0.0 0.0 0.0 4.537054807168365E-5 4.537054807168365E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 945 0.0 13.172146 1 TAGGACC 745 0.0 12.19725 4 GGCGAGG 890 0.0 11.806183 19 GCGTTAA 60 0.0046569137 11.434874 1 CGGTAGG 130 2.062552E-8 11.309215 1 TTAGGCC 80 3.9006738E-4 10.648772 3 TGTAGGA 2080 0.0 10.508145 2 GTAGGAC 2150 0.0 10.47817 3 TCGAACT 100 2.495808E-5 10.412842 19 AATGCCG 165 9.895302E-10 10.326784 18 TAGGACT 330 0.0 10.326081 4 CTGTAGG 2070 0.0 10.322164 1 AGGACGT 2115 0.0 10.025006 5 CGTGCGC 85 6.81905E-4 10.022373 10 GACGTGA 1150 0.0 9.959432 7 AGGACCG 105 4.2733875E-5 9.916317 5 GTCCTAA 1040 0.0 9.895563 1 GGACGTG 2110 0.0 9.86932 6 TGGCGAG 1920 0.0 9.860645 18 CTACACT 375 0.0 9.844197 4 >>END_MODULE