FastQCFastQC Report
Thu 26 May 2016
SRR1512041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2204073
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45090.20457580125522157No Hit
GTCCTACAGTGGACATTTCTAAATT41490.18824240394941547No Hit
CTGTAGGACGTGGAATATGGCAAGA40800.1851118361324693No Hit
GTCCTAAAGTGTGTATTTCTCATTT34660.15725431961645553No Hit
CTTTAGGACGTGAAATATGGCGAGG31680.14373389629109382No Hit
TATCAACGCAGAGTACTTTTTTTTT27820.1262208647354239No Hit
GGTATCAACGCAGAGTACTTTTTTT27070.12281807363004765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6100.012.3100434
GGTATCA11650.012.2134831
AATGCCG951.0475087E-611.99325218
AAGGCGT1406.8030204E-1011.5365556
AGGCGTG1553.1650416E-1011.0258047
GGCGAGG8500.010.94802419
TATACCG700.001485004210.8631135
AATCCCG1752.0008883E-1110.85226819
ACTGTCG700.001499130710.8507928
AGACCGT803.7694836E-410.6882786
TAGACAG3750.010.6458515
TGTAGGA22800.010.2973262
GTAGGAC21550.010.2330023
GTCCTAC20300.010.1139681
GACCGTG1054.132812E-59.9465587
CTGTAGG22500.09.9314461
TCCTACA22400.09.8871312
GTGGTAT4850.09.8448111
GTAGAAC4450.09.8256243
GGCGTGC1554.101821E-89.80071458
Produced by FastQC (version 0.11.5)