FastQCFastQC Report
Thu 26 May 2016
SRR1512040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351040
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31700.13483394582822922No Hit
GTCCTACAGTGGACATTTCTAAATT28520.12130801687763715No Hit
CTGTAGGACGTGGAATATGGCAAGA27150.11548080849326256No Hit
GTCCTAAAGTGTGTATTTCTCATTT23950.10186981080713216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG307.917896E-418.93029
CGTCTAC550.002419392312.48074151
GGTATCA11350.011.66387751
GGCGAGG8050.011.64080519
TAGGACC6300.011.5684554
GGACGTG16400.010.7348096
AGGACGT16800.010.7045775
TCCAACG1106.3165844E-610.32600318
ATAGGAC2850.010.2953723
GGCGTGC1201.5981641E-610.2538598
GACGTGA9300.010.1775277
TTAGGAC11750.010.149813
TAGGACG17950.010.0714994
TGGCGAG18250.010.06195818
AAGAGCG856.822556E-410.021875
GTCCTAC14250.09.9783381
GTATCAA21650.09.9195341
CCGTAGG1104.8303205E-59.8062971
GTATAAG2600.09.8062971
GTCCTAA9900.09.5091371
Produced by FastQC (version 0.11.5)