FastQCFastQC Report
Thu 26 May 2016
SRR1512040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351040
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40760.1733700830270859No Hit
GTCCTACAGTGGACATTTCTAAATT32940.14010820743160474No Hit
CTGTAGGACGTGGAATATGGCAAGA31480.13389818973730774No Hit
GTCCTAAAGTGTGTATTTCTCATTT28220.12003198584456241No Hit
CTTTAGGACGTGAAATATGGCGAGG25870.1100364094188104No Hit
GGTATCAACGCAGAGTACTTTTTTT25180.10710153804273853No Hit
TATCAACGCAGAGTACTTTTTTTTT24960.10616578195181707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6500.013.1589454
CCGCGCT658.060252E-411.68639217
GGTATCA10950.011.5943741
AGGACGT17100.011.3377275
GGACGTG16850.011.3323956
TTTTACG600.00586024711.0876292
CTAGGAC2850.011.0042633
GACGTGA9550.010.93687257
TTAGGAC11150.010.9100573
TGTAGGA18350.010.8761492
GTAGGAC18050.010.7936563
TAGGACG18150.010.7865494
CTGTAGG18300.010.693321
GGCGAGG8500.010.61453219
GACGTGG8550.010.5502157
TACACGG1106.0204056E-610.3676545
GTGGTAT5150.010.1944841
GTCCTAA9300.010.1615991
GTCCTAC16100.010.1386471
TGGCGAG17250.010.12842318
Produced by FastQC (version 0.11.5)