FastQCFastQC Report
Thu 26 May 2016
SRR1512039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374127
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45990.19371331019781166No Hit
GGTATCAACGCAGAGTACTTTTTTT27350.11520023991976841No Hit
TATCAACGCAGAGTACTTTTTTTTT26680.11237814994732802No Hit
GTCCTACAGTGGACATTTCTAAATT25910.10913485251631441No Hit
CTGTAGGACGTGGAATATGGCAAGA24410.10281674063771652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG551.501248E-414.2597431
TAGGACC6950.011.9849374
CGGTAGG853.8639184E-511.5336151
CGATAAA600.004649123611.4375021
GGTATCA12750.011.3798331
AGGACGT13250.011.3584515
GGACGTG13300.011.1022456
GACGTGA8000.011.0034997
GGCGAGG6850.010.77854619
TGGTATC4550.010.7731582
TGGCGAG15000.010.72787918
GTAGGAC16300.010.5687033
TAGGACG14250.010.5613674
TCCAACG2001.8189894E-1210.41235318
TGTAGGA16050.010.2616832
TTAGGAC9300.010.1778243
AATATCG750.002729880910.0964015
TATGGCG16050.010.085031516
ATGGCGA16600.09.92195717
GGCGAGA9450.09.816359519
Produced by FastQC (version 0.11.5)