FastQCFastQC Report
Thu 26 May 2016
SRR1512039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374127
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58140.24489001641445465No Hit
TATCAACGCAGAGTACTTTTTTTTT34930.14712776527961646No Hit
GGTATCAACGCAGAGTACTTTTTTT33840.14253660398116866No Hit
GTCCTACAGTGGACATTTCTAAATT28640.12063381613536259No Hit
CTGTAGGACGTGGAATATGGCAAGA27370.11528448141148304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.012.58459951
TAGGACC7700.012.0965974
TCCAACG2300.011.55944918
GTATACG700.001431796110.9107861
TGTAGGA19550.010.8414142
TATACGA803.755892E-410.6925292
CCAACGA1605.475158E-1010.683743519
GTATCAA28550.010.399641
GTAGGAC18300.010.2315863
AGGACGT14250.010.2047995
GGACGTG14750.010.1119326
CTGTAGG19750.010.0544961
GTCCTAC15400.010.0428811
TGGCGAG16250.09.93350418
GGCGAGG7550.09.8111219
GGCGTGC1554.0998202E-89.80114658
TAGGACG15750.09.7760264
AGGACCT13150.09.757445
GACGTGG8000.09.7322327
ACTGTTC6850.09.7027788
Produced by FastQC (version 0.11.5)