FastQCFastQC Report
Thu 26 May 2016
SRR1512038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404594
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38750.16114986563220238No Hit
TATCAACGCAGAGTACTTTTTTTTT24140.10039116790609974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG250.0061593518.93194210
GGTATCA9750.012.3614371
TAGGACC6250.012.2678984
CGGTCAA550.00315287612.04759910
AGTCGTC1053.6205038E-610.8182538
AGGACGT17100.010.6838155
TAGAACG909.871724E-510.5177464
GGACGTG16750.010.5114676
GTAGGAC18200.010.4541763
TTAGGAC11750.010.3118673
TCCAACG1304.0365194E-710.19433418
GACGTGA10200.010.1155967
TAGGACG18650.010.1004194
GTATCAA21100.010.0773361
TGTAGGA18950.09.9796782
GGCGAGG7500.09.97102919
AGGATCG1054.2718646E-59.9167315
GACGTGG7750.09.8934667
GTCCTAC13950.09.833821
CGGTAGG1002.0187923E-49.7986991
Produced by FastQC (version 0.11.5)