FastQCFastQC Report
Thu 26 May 2016
SRR1512038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404594
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49500.20585595738823267No Hit
TATCAACGCAGAGTACTTTTTTTTT30230.12571768872416716No Hit
GGTATCAACGCAGAGTACTTTTTTT29240.1216005695764025No Hit
GTCCTACAGTGGACATTTCTAAATT27820.11569520675839663No Hit
CTGTAGGACGTGGAATATGGCAAGA26160.10879175445002358No Hit
GTCCTAAAGTGTGTATTTCTCATTT24870.10342702343929995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6200.012.7230644
GGTATCA12500.012.6740351
GTCCTAG3050.010.9517891
GACCGTC700.001498114110.8517487
CTACACG803.7577355E-410.6919724
TGTAGGA16950.010.4852112
GTAGGAC16600.010.4200213
GTCCTAA8200.010.3584071
GTATCAA26700.010.2228411
TGGACCG1502.4305336E-810.1375745
GAACCGC750.002647052810.1339876
GTCCTAC15900.010.0839111
AGGACGT15650.010.08089355
GGACGTG15650.010.0773296
TAAAGGT3400.010.0630324
AGGCGTG1803.9835868E-1010.0227967
AGGACCT13000.09.942625
GGCGAGG6550.09.8587219
TCCTACA17750.09.8520082
CTGTAGG18150.09.7277411
Produced by FastQC (version 0.11.5)