FastQCFastQC Report
Thu 26 May 2016
SRR1512037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719340
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34650.20153081996580083No Hit
GGTATCAACGCAGAGTACTTTTTTT20740.1206276827154606No Hit
TATCAACGCAGAGTACTTTTTTTTT18880.10980957809391975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG400.005411029314.1971945
GGCGAGA4050.012.15374619
TAGGACC3700.011.7670454
TGGCGAG5500.011.70329218
GGTATCA6900.011.6550971
TATGGCG5400.011.39413616
ATGGCGA5900.010.90984817
GTAGGAC9300.010.88960553
GACGAAA700.001538448510.81691111
TCCTACA9000.010.7880732
GTCCTAC8100.010.7759641
ACCGTGC1106.319011E-610.32523258
GCGTGCG1106.319011E-610.32523259
GTATCAA14850.010.2366151
GGACGTG6650.010.1052726
TGTAGGA10250.09.950862
GTCCTAA4350.09.9200661
CGTGCGC1054.2757587E-59.91550210
CTACAGT10950.09.8537614
CCTACAG10300.09.8323623
Produced by FastQC (version 0.11.5)