##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512037_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1719340 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.251773354891995 31.0 30.0 34.0 23.0 34.0 2 26.313761094373422 31.0 25.0 34.0 10.0 34.0 3 28.27450242534926 31.0 27.0 34.0 16.0 34.0 4 32.748481975641816 35.0 33.0 37.0 26.0 37.0 5 33.19562913676178 35.0 35.0 37.0 27.0 37.0 6 33.48053380948504 37.0 35.0 37.0 28.0 37.0 7 33.54611595146975 37.0 35.0 37.0 28.0 37.0 8 33.58234206148871 37.0 35.0 37.0 28.0 37.0 9 35.18161387509161 39.0 35.0 39.0 29.0 39.0 10 35.088852699291586 39.0 35.0 39.0 28.0 39.0 11 35.1055050193679 39.0 35.0 39.0 28.0 39.0 12 34.99377842660556 39.0 35.0 39.0 27.0 39.0 13 34.988929472937286 39.0 35.0 39.0 27.0 39.0 14 36.17297218700199 40.0 36.0 41.0 27.0 41.0 15 36.10732083241244 40.0 36.0 41.0 27.0 41.0 16 36.052329382204796 40.0 36.0 41.0 27.0 41.0 17 36.01285435108821 40.0 36.0 41.0 27.0 41.0 18 35.95614828945991 40.0 36.0 41.0 27.0 41.0 19 35.97943978503379 40.0 36.0 41.0 27.0 41.0 20 35.87809333814138 40.0 36.0 41.0 27.0 41.0 21 35.81725662172694 40.0 36.0 41.0 27.0 41.0 22 35.71312131399258 40.0 36.0 41.0 27.0 41.0 23 35.62080391312946 40.0 36.0 41.0 27.0 41.0 24 35.51927949096746 40.0 36.0 41.0 26.0 41.0 25 35.41555015296567 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3120730981003668 1101 2 4.181196827109961 1101 3 2.2634358256525395 1101 4 1.4367325029551026 1101 5 1.0761860040787283 1101 6 0.8119423991847938 1101 7 0.3926097273984226 1101 8 0.35391955930531793 1101 9 0.4379845775745821 1101 10 0.5884911866338953 1101 11 0.6985622488646328 1101 12 0.4008043085954185 1101 13 0.5216394823556172 1101 14 0.5370968678002868 1101 15 0.5364561167472957 1101 16 0.5336528110489027 1101 17 0.6217380212334831 1101 18 0.8056000258444485 1101 19 0.6241160435729824 1101 20 0.46618768803542565 1101 21 0.43591089328302246 1101 22 0.5625726982754742 1101 23 0.6716170704183355 1101 24 0.7070851863052212 1101 25 0.3794169775077023 1102 1 1.6405655207104353 1102 2 4.374172478909141 1102 3 2.722081232533238 1102 4 1.9159137139010198 1102 5 1.3577373938654134 1102 6 1.1330685932521618 1102 7 1.0003436927794453 1102 8 0.9718697137260648 1102 9 1.0425640503893518 1102 10 0.8039504985637933 1102 11 1.052733045388372 1102 12 1.0226587939793106 1102 13 1.0418578261033744 1102 14 1.347537146475858 1102 15 1.1759705892029473 1102 16 1.2126444076875558 1102 17 1.1654484284733044 1102 18 1.2162529023796793 1102 19 1.2857119639924335 1102 20 1.3057826029244595 1102 21 1.2849865235351174 1102 22 1.2976412898146563 1102 23 1.1567826238191827 1102 24 1.2568230301607102 1102 25 1.2916830121266827 1103 1 1.408307132122932 1103 2 4.160308705551696 1103 3 2.485386436471355 1103 4 1.7438401756249533 1103 5 1.3279182256918176 1103 6 1.2201371084219446 1103 7 0.9104662565728958 1103 8 0.8566537201556415 1103 9 0.9410591277451914 1103 10 0.7832131065605807 1103 11 1.0330565334333812 1103 12 0.8395223664125098 1103 13 0.9650243923079103 1103 14 1.0330424709685246 1103 15 1.0843712321605494 1103 16 0.8995341002734492 1103 17 0.9380885731387636 1103 18 1.01929087706322 1103 19 1.1567889179807764 1103 20 1.0619369127496512 1103 21 1.2306827260667674 1103 22 1.113255113403163 1103 23 1.3933729392702574 1103 24 1.4807430623085907 1103 25 1.347455565873922 1104 1 1.4331600802194941 1104 2 3.9818198501252873 1104 3 2.3053360858996186 1104 4 1.6512084563333076 1104 5 1.1853139560888124 1104 6 1.1666698206080355 1104 7 0.8216265156979574 1104 8 0.9336281370458224 1104 9 0.8438362519589475 1104 10 0.8998813417808833 1104 11 0.8145091709563204 1104 12 0.9589826271061241 1104 13 0.9736451630951706 1104 14 1.00823579520425 1104 15 0.9644027600886531 1104 16 1.116379582054705 1104 17 0.9928444277652133 1104 18 1.015286359628746 1104 19 1.0730430550409977 1104 20 0.961944755517834 1104 21 1.0655069696317767 1104 22 1.172129989879565 1104 23 1.190449939568282 1104 24 1.349258269436099 1104 25 1.4352647670095493 1105 1 1.4594895773590437 1105 2 4.271064409334006 1105 3 2.419938850630377 1105 4 1.6490588521958571 1105 5 1.1720059995112635 1105 6 0.920970883908943 1105 7 0.8433852287551176 1105 8 0.7666097574481441 1105 9 0.789164183456549 1105 10 0.6353657632724747 1105 11 0.7810630520417305 1105 12 0.8234039702933558 1105 13 0.714588555121523 1105 14 0.7744053149009105 1105 15 0.7515318476384536 1105 16 0.7320627139322085 1105 17 1.0987760589266813 1105 18 0.9475103874096149 1105 19 1.0496222386811525 1105 20 1.0056343280456446 1105 21 0.9764835295231435 1105 22 1.033405950968536 1105 23 0.900759432107364 1105 24 1.0155531888908698 1105 25 0.9505405351758185 1106 1 0.6390928257359363 1106 2 2.4154824324934623 1106 3 1.2665978791437489 1106 4 0.7315300109613574 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0.18461051093960634 2314 6 0.004836110787209691 2314 7 -0.1423680317665088 2314 8 -0.10622241048670844 2314 9 -0.2815447175252004 2314 10 -0.24960485458939985 2314 11 -0.06254350629818362 2314 12 -0.308383656023409 2314 13 -0.21504526208956776 2314 14 -0.3210588817670583 2314 15 -0.2552526216769593 2314 16 -0.4082902241347668 2314 17 -0.2376959700413721 2314 18 -0.35969609222551924 2314 19 -0.2854565982501853 2314 20 -0.16819016617337468 2314 21 -0.37525869470911744 2314 22 -0.22743900942945317 2314 23 -0.36925052345967657 2314 24 -0.2484978165039351 2314 25 -0.33902080315780836 2315 1 -2.707113708959316 2315 2 -10.908675636640396 2315 3 -4.664469583685822 2315 4 -2.1659594524271206 2315 5 -1.1684372845367506 2315 6 -0.618683456698939 2315 7 -0.5113526887747568 2315 8 -0.5382799891250585 2315 9 -0.8069251544553069 2315 10 -0.7959848057316989 2315 11 -0.88921534850315 2315 12 -0.6318646394066789 2315 13 -0.6398769215081543 2315 14 -0.9621642000975257 2315 15 -1.2002319233976664 2315 16 -1.0055681854065526 2315 17 -1.0632042458665723 2315 18 -0.9502873819805089 2315 19 -0.9407004335100169 2315 20 -0.8594080989487125 2315 21 -1.0072409646165355 2315 22 -1.275499460956425 2315 23 -1.0778340917454017 2315 24 -1.1152524983129197 2315 25 -1.0865596719927808 2316 1 -0.7264307772762777 2316 2 -0.2484960134079124 2316 3 -0.2890836338392937 2316 4 -0.6457232859468434 2316 5 -0.9428924555184324 2316 6 -1.0722217358720343 2316 7 -1.2207344551996613 2316 8 -1.2607566872070493 2316 9 -1.282284065640873 2316 10 -1.5351904315036506 2316 11 -1.8213660468576833 2316 12 -1.570588328085698 2316 13 -1.6805926024173417 2316 14 -1.8744964252062744 2316 15 -1.659613365358645 2316 16 -1.745000503311985 2316 17 -1.8607313980899107 2316 18 -1.865371926465599 2316 19 -1.7276355688439224 2316 20 -1.766434040921581 2316 21 -1.775515530321563 2316 22 -1.7907546734694861 2316 23 -1.842247752974771 2316 24 -1.9714846933546823 2316 25 -1.858448333276229 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65332.0 3 2.0 4 527.0 5 2686.0 6 2917.0 7 2689.0 8 2531.0 9 2530.0 10 2383.0 11 2318.0 12 2307.0 13 2355.0 14 2358.0 15 2464.0 16 2496.0 17 2771.0 18 3209.0 19 4024.0 20 5224.0 21 6609.0 22 8135.0 23 9575.0 24 11098.0 25 12829.0 26 14300.0 27 16265.0 28 18635.0 29 22319.0 30 28180.0 31 35387.0 32 45795.0 33 60354.0 34 83113.0 35 120300.0 36 193248.0 37 268285.0 38 653790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80390149708609 18.236648946688845 12.605592843765631 25.353856712459432 2 17.653851206956194 20.876780421599978 38.27016003153141 23.19920833991242 3 19.46496911605616 23.12276803889865 29.18015052287506 28.23211232217013 4 12.53079670105971 16.017308967394463 37.61274675166052 33.83914757988531 5 13.627729244942827 37.128665650772966 35.51217327579187 13.73143182849233 6 32.982132678818616 36.51372038107646 17.391557225446974 13.112589714657949 7 28.960473204834415 31.191387392836784 21.1842916467947 18.6638477555341 8 25.71297790195994 36.736464848680136 19.717763464775395 17.83279378458453 9 25.981074133097582 15.984563844265823 19.76078029941722 38.27358172321937 10 14.750328324040998 29.24643089375097 33.983893800986195 22.019346981221844 11 35.12917747507765 22.694289669291706 23.355182802703364 18.821350052927286 12 23.02354391801505 24.56442588435097 31.166028825014248 21.246001372619727 13 28.903823560203335 21.394953877650728 25.639140600462966 24.06208196168297 14 21.177440865355816 21.422302408076593 27.340216210997365 30.06004051557023 15 23.292716972791887 29.797713075947748 23.253283236590786 23.656286714669584 16 22.783335465934602 28.4710993753417 26.39332534577222 22.35223981295148 17 22.16257401095769 28.215361708562586 27.207358637616757 22.41470564286296 18 22.079434292251705 27.742502470434754 28.46865615910356 21.70940707820998 19 23.443738807406845 27.088009333873853 27.35939427532485 22.108857583394453 20 23.32974473240495 27.111676446622035 27.913731662220176 21.644847158752842 21 23.352443946305712 26.601519581641014 27.103529424081703 22.942507047971574 22 23.028894082174634 27.242376901485127 27.501491874084667 22.227237142255575 23 22.86497483894833 27.043128578441294 27.402979310473185 22.68891727213719 24 22.84953694413958 26.760749334482615 27.98098571602226 22.408728005355545 25 22.96168167441579 27.03459595401475 27.56311455482181 22.440607816747647 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 107.0 1 107.0 2 258.0 3 409.0 4 409.0 5 409.0 6 1018.5 7 1628.0 8 1628.0 9 1628.0 10 2646.5 11 3665.0 12 3665.0 13 3665.0 14 5945.5 15 8226.0 16 8226.0 17 8226.0 18 13730.0 19 19234.0 20 19234.0 21 19234.0 22 29216.5 23 39199.0 24 39199.0 25 39199.0 26 54946.0 27 70693.0 28 70693.0 29 70693.0 30 91041.0 31 111389.0 32 111389.0 33 111389.0 34 129370.5 35 147352.0 36 147352.0 37 147352.0 38 164368.5 39 181385.0 40 181385.0 41 181385.0 42 195019.5 43 208654.0 44 208654.0 45 208654.0 46 216710.5 47 224767.0 48 224767.0 49 224767.0 50 222692.0 51 220617.0 52 220617.0 53 220617.0 54 201868.0 55 183119.0 56 183119.0 57 183119.0 58 158686.0 59 134253.0 60 134253.0 61 134253.0 62 110007.5 63 85762.0 64 85762.0 65 85762.0 66 65729.0 67 45696.0 68 45696.0 69 45696.0 70 33422.5 71 21149.0 72 21149.0 73 21149.0 74 14570.0 75 7991.0 76 7991.0 77 7991.0 78 5385.0 79 2779.0 80 2779.0 81 2779.0 82 1829.5 83 880.0 84 880.0 85 880.0 86 573.0 87 266.0 88 266.0 89 266.0 90 176.0 91 86.0 92 86.0 93 86.0 94 53.0 95 20.0 96 20.0 97 20.0 98 17.0 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4921539660567427 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.1632370560796585E-4 9 0.0 10 1.1632370560796585E-4 11 0.0 12 0.0 13 0.0 14 5.816185280398292E-5 15 0.0 16 0.0 17 0.0 18 4.071329696278805E-4 19 6.397803808438122E-4 20 4.071329696278805E-4 21 0.002966254493003129 22 0.001512208172903556 23 9.305896448637268E-4 24 5.816185280398292E-5 25 3.4897111682389756E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1719340.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.99594499013011 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4479768399595 66.64595701127392 2 10.43656798340339 16.280199646385682 3 2.3295889571250163 5.45095476448612 4 0.7868323242218801 2.4547892270586393 5 0.3369776513347109 1.314144517820268 6 0.1835601170313559 0.8590166874215689 7 0.12182881175655917 0.6651507309984255 8 0.06635903351183932 0.4140588421910097 9 0.05556603561838261 0.3900532911669883 >10 0.2167221750128619 2.832654805948744 >50 0.010942494241469819 0.5896261669366244 >100 0.005797832178201215 0.9484186041219971 >500 7.52790943968756E-4 0.4464074159400642 >1k 5.269536607781292E-4 0.7085682882499927 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3465 0.20153081996580083 No Hit GGTATCAACGCAGAGTACTTTTTTT 2074 0.1206276827154606 No Hit TATCAACGCAGAGTACTTTTTTTTT 1888 0.10980957809391975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.816185280398292E-5 0.0 0.0 0.0 0.0 6 1.1632370560796585E-4 0.0 0.0 0.0 0.0 7 1.1632370560796585E-4 0.0 0.0 0.0 0.0 8 1.1632370560796585E-4 0.0 0.0 0.0 0.0 9 1.1632370560796585E-4 0.0 0.0 0.0 0.0 10 1.1632370560796585E-4 0.0 0.0 0.0 0.0 11 1.1632370560796585E-4 0.0 0.0 0.0 0.0 12 1.1632370560796585E-4 0.0 0.0 0.0 0.0 13 1.1632370560796585E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGGCG 40 0.0054110293 14.197194 5 GGCGAGA 405 0.0 12.153746 19 TAGGACC 370 0.0 11.767045 4 TGGCGAG 550 0.0 11.703292 18 GGTATCA 690 0.0 11.655097 1 TATGGCG 540 0.0 11.394136 16 ATGGCGA 590 0.0 10.909848 17 GTAGGAC 930 0.0 10.8896055 3 GACGAAA 70 0.0015384485 10.816911 11 TCCTACA 900 0.0 10.788073 2 GTCCTAC 810 0.0 10.775964 1 ACCGTGC 110 6.319011E-6 10.3252325 8 GCGTGCG 110 6.319011E-6 10.3252325 9 GTATCAA 1485 0.0 10.236615 1 GGACGTG 665 0.0 10.105272 6 TGTAGGA 1025 0.0 9.95086 2 GTCCTAA 435 0.0 9.920066 1 CGTGCGC 105 4.2757587E-5 9.915502 10 CTACAGT 1095 0.0 9.853761 4 CCTACAG 1030 0.0 9.832362 3 >>END_MODULE