FastQCFastQC Report
Thu 26 May 2016
SRR1512037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719340
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43540.2532367071085416No Hit
GGTATCAACGCAGAGTACTTTTTTT27280.15866553444926543No Hit
TATCAACGCAGAGTACTTTTTTTTT25450.14802191538613654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA1750.012.5461571
CCCTATA1301.9645086E-1012.4269022
GGTATCA8750.012.2188651
TCCAACG1001.9402232E-611.39488718
CCAACGA700.001496044110.8532219
GTCCTAC8200.010.7101341
TCTTATA1803.45608E-1110.5588052
GTATCAA18750.010.3860361
TGCACCG750.00264106110.1364545
GTGTAAG2450.010.1304371
GTAGGAC8200.010.0823723
TAGGACC3650.09.8934564
ATGGCGA7300.09.88568817
TGTAGGA8900.09.8232482
TGGCGAG7000.09.76704618
TATGGCG7250.09.69192116
GTGGTAT3550.09.6804391
GTCTTAT1909.622454E-109.5459891
TAGACAG3100.09.5029255
TTAACAC1901.0459189E-99.5029253
Produced by FastQC (version 0.11.5)