FastQCFastQC Report
Thu 26 May 2016
SRR1512036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849037
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35670.1929112289261924No Hit
CTGTAGGACGTGGAATATGGCAAGA33410.18068865036232373No Hit
GTCCTACAGTGGACATTTCTAAATT32960.17825495109075698No Hit
GTCCTAAAGTGTGTATTTCTCATTT31720.17154875754243967No Hit
CTTTAGGACGTGAAATATGGCGAGG31450.1700885379794996No Hit
TATCAACGCAGAGTACTTTTTTTTT22270.12044107283953756No Hit
GGTATCAACGCAGAGTACTTTTTTT21550.11654715400503071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG450.00933708213.0736741
CGCTAGA450.00933708213.0736741
CGGTAGG751.0680056E-513.0736741
GGTATCA7800.012.9479661
TAGGACC5550.012.790994
GTATAAG2100.011.6729231
TGGCGAA855.525839E-511.13568518
GGCGAGG10700.010.96917119
GGACGTG20200.010.9647916
GGACGGG951.4167155E-510.9598596
TCCGTGT951.4167155E-510.9598598
AGGACGT20400.010.9036925
GACGTGA11600.010.8524937
CCAACGT700.001537817510.81752219
GGCGAAA700.001537817510.81752219
TTAGGAC13000.010.7031073
AGGACCT12000.010.5696225
GTCCTAT2051.8189894E-1210.5227131
GACGTGG9300.010.483117
GTCCTAG3000.010.458941
Produced by FastQC (version 0.11.5)